Suchergebnisse

Cover -- Title Page -- Copyright -- Contents -- Preface -- Acknowledgments -- Abbreviations -- Chapter 1 Introduction -- 1.1 Exploring the Microbial World -- 1.2 The DNA Sequencing Revolution: Historical Perspectives -- References -- Chapter 2 The Architecture of Microbial Genomes -- Introduction -- 2.1 Genome Size, Organization, and Replication -- 2.2 Nucleotide Composition -- 2.3 Ecological and Evolutionary Aspects of Microbial Genomes -- 2.3.1 The Role of Viruses in Promoting Genomic Diversity -- 2.4 Genomic Diversity in Microbial Communities -- 2.5 Does Genomic Diversity Matter? -- References -- Chapter 3 Application of Omics Approaches to Earth and Environmental Sciences: Opportunities and Challenges -- Introduction -- 3.1 New Perspectives on Microbial Biogeochemistry -- 3.1.1 Redefining the Carbon and Nitrogen Cycles -- 3.1.2 Omics as Sensitive and Efficient Tracers of Biogeochemical Processes -- 3.1.3 Omics Data is Valuable for Biogeochemical Models -- 3.1.4 Understanding Biotic Responses and Feedbacks to Global Change -- 3.2 A Genomic Record of Biological and Geochemical Evolution -- 3.3 Challenges and Limitations of Omics Approaches -- 3.4 Omics as a Complement to Other Approaches -- References -- Chapter 4 Overview of Approaches: From Whole-Community Shotgun Sequencing to Single-Cell Genomics -- Introduction -- 4.1 Choosing the Right Approach -- 4.1.1 Whole-Community Approaches -- 4.1.2 Targeted Approaches: Physical, Microbiological, and Isotopic Enrichment -- 4.1.3 Single-Cell Genomics -- 4.2 Experimental Design and Sampling Considerations -- 4.2.1 Replication -- 4.2.2 Estimating Sequencing Effort: How Much Sequencing Do I Need to Do? -- 4.2.3 From Sample to Data: Biases Due to Preservation, Storage, Extraction, and Sequencing -- 4.2.4 Estimating Absolute Abundance with Internal Standards