Suchergebnisse

The recent boom in phage therapy and phage biocontrol requires the design of suitable cocktails of genetically different bacteriophages. The current methods for typing phages need significant quantities of purified DNA, may require a priori genetic information and are cost and time consuming. We have evaluated the randomly amplified polymorphic DNA (RAPD)-PCR technique to produce unique and reproducible band patterns from 26 different bacteriophages infecting Staphylococcus epidermidis, Staphylococcus aureus, Lactococcus lactis, Escherichia coli, Streptococcus thermophilus, Bacillus subtilis and Lactobacillus casei bacterial strains. Initially, purified DNA and phage suspensions of seven selected phages were used as a template. The conditions that were found to be optimal 8 μM of 10-mer primers, 3 μM magnesium oxalacetate and 5% dimethyl sulfoxide. The RAPD genomic fingerprints using a phage titer suspension higher than 109 PFU mL−1 were highly reproducible. Clustering by the Pearson correlation coefficient and the unweighted pair group method with arithmetic averages clustering algorithm correlated largely with genetically different phages infecting the same bacterial species, although closely related phages with a similar DNA restriction pattern were indistinguishable. The results support the use of RAPD-PCR for quick typing of phage isolates and preliminary assessment of their genetic diversity bypassing tedious DNA purification protocols and previous knowledge of their sequence. ; This work was supported by grants AGL2009-13144-C02-01 from the Ministry of Education of Spain, IB08-052 from FICYT (Regional Government of Asturias) and PIE200970I090 (CSIC, Spain). ; Peer reviewed

3 Páginas.-- 1 Tabla ; The draft genome sequences of five Lactobacillus pentosus strains isolated from biofilms on the skin of green table olives are presented here. These genome sequences will assist in revealing the potential probiotic properties of these strains, as the intake of fermented olives implicates the passage of millions of Lactobacillus spp. throughout a consumer's gastrointestinal tract. ; This work was funded by the Junta de Andalucía through the P11-AGR-7051 project and by the Spanish Government through MICIIN projects AGL2013-48300-R/ALI (OliFilm project: www.olifilm.science.com.es) and BFU2016-77297-P. B.C.-D. was the beneficiary of a PIF grant from the Junta de Andalucía, and A.B.-C. was supported by the MICIIN through a PIF grant. We thank C3UPO for the high-performance computing (HPC) support. ; Peer reviewed

Here, we report the genome sequences of six Lactobacillus pentosus strains isolated from traditional noninoculated Spanish-style green table olive brines. The total genome sizes varied between 3.77 and 4.039 Mbp. These genome sequences will assist in revealing the genes responsible for both technological and probiotic properties of these strains. ; The Junta de Andalucía supported this study through the P11-AGR-7051 project and the Spanish Government through MICIIN projects AGL2013-48300-R/ALI (OliFilm project) and BFU2016-77297-P. B.C.-D. was the beneficiary of a PIF grant from the Junta de Andalucía. We thank C3UPO for the HPC support. ; Peer Reviewed