Synthesis of labelled PNA oligomers by a post-synthetic modification approach

Abstract

The preparation of t-butoxycarbonyl (Boc)-protected O4-(o-nitrophenyl) thymine peptide nucleic acid (PNA) monomer is described. This PNA monomer was incorporated into PNA oligomer sequences. The post-synthetic modification of the oligomers to yield fluorescently-labelled PNA oligomers was studied before and after the removal of the protecting groups. In both cases, the desired fluorescently-labelled PNA oligomer was obtained in good yields. ; This work was supported by the Commission of the European Union as part of the Information Societies Technology Programme (IST-1999-11974) by the Dirección General de Investigación Científica y Técnica (grants BQU2000-0649) and the Generalitat de Catalunya (2000-SGR-0018 and 2001-SGR-0049). ; Peer reviewed