Small ocean temperature increases elicit stage-dependent changes in DNA methylation and gene expression in a fish, the European sea bass

Abstract

12 pages, 4 figures, 1 table, supplementary material https://dx.doi.org/10.1038/s41598-017-10861-6 ; In natural fish populations, temperature increases can result in shifts in important phenotypic traits. DNA methylation is an epigenetic mechanism mediating phenotypic changes. However, whether temperature increases of the magnitude predicted by the latest global warming models can affect DNA methylation is unknown. Here, we exposed European sea bass to moderate temperature increases in different periods within the first two months of age. We show that increases of even 2 °C in larvae significantly changed global DNA methylation and the expression of ecologically-relevant genes related to DNA methylation, stress response, muscle and organ formation, while 4 °C had no effect on juveniles. Furthermore, DNA methylation changes were more marked in larvae previously acclimated to a different temperature. The expression of most genes was also affected by temperature in the larvae but not in juveniles. In conclusion, this work constitutes the first study of DNA methylation in fish showing that temperature increases of the magnitude predicted by the latest global warming models result in stage-dependent alterations in global DNA methylation and gene expression levels. This study, therefore, provides insights on the possible consequences of climate change in fish mediated by genome-wide epigenetic modifications ; DA and ND were supported by Ph.D. scholarships from the Spanish Government (BES-2011-044860 and BES-2007-14273, respectively). Research was supported by the Ministry of Economy and Competitiveness "Epifarm" (AGL2013-41047-R) to FP ; Peer Reviewed