Cryo-electron microscopy at the Faculty of Biology of Lomonosov Moscow State University

In: Vestnik Moskovskogo universiteta. Seria 16. Biologia, Band 78, Heft №3s, 2023, S. 51-56

Abstract

This paper demonstrates an example of a successful upgrade of a JEOL JEM-2100 analytical
transmission electron microscope to a low-resolution cryo-electron microscope designed for
routine tasks of sample preparation and quality evaluation. As a result of the upgrade, the instrument allows the subnanometer resolution of protein molecule reconstructions (within 8 Å).
The influence of graphene and amorphous carbon support films to prevent the effect of preferred
orientation of protein particles in the frozen sample is discussed.