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In: Techniques in Visible and Ultraviolet Spectometry 2
In: Techniques in Visible and Ultraviolet Spectrometry 2
1 General considerations on fluorescence spectrometry -- 1.1 Introduction -- 1.2 Molecular photochemistry -- 1.3 Fluorescence instrumentation -- 1.4 Good spectroscopic practice -- 1.5 Fluorescence intensities -- 1.6 Nomenclature in fluorescence spectrometry -- 2 Monochromator wavelength calibration -- 2.1 Introduction -- 2.2 Characteristics of calibration methods -- 2.3 Use of spectral lines from the spectrometer light source -- 2.4 Use of an auxiliary light source -- 2.5 Use of narrow bandwidth fluorescence maxima of inorganic and organic solutes -- 2.6 Conclusions and recommendations -- 3 Stray light in fluorescence spectrometers -- 3.1 Origins of stray light and resultant errors -- 3.2 Stray light in grating monochromators -- 3.3 Summary and recommendations -- 4 Criteria for fluorescence spectrometer sensitivity -- 4.1 Background: inter-instrument comparisons -- 4.2 The limit of detection method -- 4.3 The signal-to-noise ratio method -- 4.4 Summary and recommendations -- 5 Inner filter effects, sample cells and their geometry in fluorescence spectrometry -- 5.1 Inner filter effects -- 5.2 Sample cells -- 5.3 Recommendations -- 6 Temperature effects and photodecomposition in fluorescence spectrometry -- 6.1 Errors caused by temperature effects -- 6.2 Countermeasures and recommendations for temperature effects -- 6.3 Errors caused by photolysis effects -- 6.4 Countermeasures and recommendations -- 7 Correction o excitation and emission spectra -- 7.1 Introduction: the need for correction procedures -- 7.2 Excitation spectra -- 7.3 Emission spectra -- 7.4 Polarization effects -- 7.5 Recommendations -- 8 The determination of quantum yields -- 8.1 Introduction -- 8.2 Primary methods of determining quantum yields -- 8.3 Secondary methods of determining quantum yields: use of fluorescence standards -- 8.4 Other methods of determining quantum yields -- 8.5 Summary and recommendations -- Appendix Corrected excitation and emission spectra.
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