Viroporins are viral proteins with ion channel (IC) activity that play an important role in several processes, including virus replication and pathogenesis. While many coronaviruses (CoVs) encode two viroporins, severe acute respiratory syndrome CoV (SARS-CoV) encodes three: proteins 3a, E, and 8a. Additionally, proteins 3a and E have a PDZ-binding motif (PBM), which can potentially bind over 400 cellular proteins which contain a PDZ domain, making them potentially important for the control of cell function. In the present work, a comparative study of the functional motifs included within the SARS-CoV viroporins was performed, mostly focusing on the roles of the IC and PBM of E and 3a proteins. ; This work was supported by grants from the Government of Spain (BIO2013-42869-R and BIO2016-75549-R AEI/FEDER, UE), the European Zoonotic Anticipation and Preparedness Initiative (ZAPI) (IMI_JU_115760), and the U.S. National Institutes of Health (NIH) (0258-3413/HHSN266200700010C awarded to L.E., 2P01AI060699 awarded to L.E. and S.P., and R01 AI129269 awarded to S.P.). V.M.A. and M.Q.M. are grateful for the support of the Government of Spain (FIS2013-40473-P and FIS2016-75257-P AEI/FEDER, UE) and Universitat Jaume I (P1.1B2015-28). C.C.R. received a contract from Fundación La Caixa.
The article deals with general and particular patterns of solving the identification problem of attributional linguistics. Experimental research is carried out on the material of the texts of written network communication. The problem is solved for several discursive areas: online literature, corporate e-mail, short comments on posts on the entertainment portal. These discursive spheres correlate with several functional styles of speech: fiction, official and colloquial, which makes it possible to identify the trends in textual attribution within the material of Internet communication. A holistic approach with linguistic modelling as its means, is used for the analysis of speech material. The problem of determining text authorship is solved by comparing a disputed text, the author of which, according to the conditions of the experiment, is unknown, with texts, the authors of which are known. The application of specialized software "KhoRom", has enabled the researchers to create mathematical models of the individual styles of the compared texts authors. The contrast of the resulting models is performed by considering Pearson linear correlation coefficient, coefficient of determination, and Students t-criterion. On the basis of these models and their study, a number of conclusions about statistical patterns of an authorship study in various discursive spheres of electronic communication are made. The revealed general and particular statistical regularities are represented in score tables.
50°31'29.7"N 22°46'39.1"E 50°30'56.2"N 22°46'01.0"E 50°30'41.0"N 22°45'49.5"EThis richly illustrated text is a descriptive introduction to Dominika Macocha's video-sculptural installation, detailing the idea behind the work, the process of its creation, and its suggested interpretations. The file is concluded with a link to the film The Mystery of Forest Lakelet.50°31'29.7"N 22°46'39.1"E 50°30'56.2"N 22°46'01.0"E 50°30'41.0"N 22°45'49.5"EBogato ilustrowany tekst stanowi opis wstępny instalacji wideo-rzeźbiarskiej Dominiki Macochy - pokazuje zamysł tej pracy, proces jej powstawania i proponowane interpretacje. Na końcu zamieszczony jest link do filmu Tajemnica leśnego jeziorka.