Suchergebnisse

In recent years, trafficking and abuse of hallucinogenic mushrooms have become a serious social problem. It is therefore imperative to identify hallucinogenic mushrooms of the genus Psilocybe for national drug control legislation. An internal transcribed spacer (ITS) is a DNA barcoding tool utilized for species identification. Many methods have been used to discriminate the ITS region, but they are often limited by having a low resolution. In this study, we sought to analyze the ITS and its fragments, ITS1 and ITS2, by using high-resolution melting (HRM) analysis, which is a rapid and sensitive method for evaluating sequence variation within PCR amplicons. The ITS HRM assay was tested for specificity, reproducibility, sensitivity, and the capacity to analyze mixture samples. It was shown that the melting temperatures of the ITS, ITS1, and ITS2 of Psilocybe cubensis were 83.72 ± 0.01, 80.98 ± 0.06, and 83.46 ± 0.08 °C, and for other species, we also obtained species-specific results. Finally, we performed ITS sequencing to validate the presumptive taxonomic identity of our samples, and the sequencing output significantly supported our HRM data. Taken together, these results indicate that the HRM method can quickly distinguish the DNA barcoding of Psilocybe cubensis and other fungi, which can be utilized for drug trafficking cases and forensic science.

Why do some near crises tip over into full-blown crisis and others do not? This paper considers existing scholarship and identifies four key barriers to using quantitative analysis for tipping-point analyses: strategic indeterminacy; the incentives for conflict parties to avoid inefficiencies; the paucity of cases; and the availability of quality data. Due to these challenges, many do not perform well as immediate causes for crisis escalation. We also argue and demonstrate through two quantitative models of crisis escalation that some variables, particularly related to domestic politics, can do well in explaining why some disputes tip into crisis and others do not. As we illustrate with reference to the 1995–1996 Third Taiwan Straits Crisis, qualitative approaches that analyze the processes by which leaders and foreign policy institutions make decisions add needed explanatory power to purely quantitative models of the potential for near crises to tip into crisis.