β-Galactosidase from Aspergillus oryzae is an enzyme with a wide industrial application, mostly in the hydrolysis of lactose and, more recently, in the synthesis of oligosaccharides. Several advantages are associated with the application of immobilized enzymes on magnetic supports. In this work, β-galactosidase was covalently immobilised onto a Polysiloxane- Polyvinyl Alcohol Magnetic Composite (mPOS-PVA), Magnetic Polysiloxane with Polyaniline (mPOS-PANI), Magnetized Dacron (DACRON) and Magnetite with Polyaniline (MAG-PANI) using glutaraldehyde as activating agent being the synthesis of GOS evaluated and compared at different temperatures (30, 40, 50 and 60 oC) and various initial lactose concentration (50, 100, 200, 300, 400 and 500 g/L). The kinetic parameters obtained by fitting the experimental data were compared in order to determine the effect of the immobilization process with different supports on the synthesis of oligosaccharides. These results clearly demonstrate that all supports may be used for β-galactosidase immobilization as, besides improving the enzyme hydrolytic and GOS synthesis properties, its separation from the obtained reaction products is easy to accomplish. ; Programme Alβan, European Union Programme of High Level Scholarships for Latin America ; Brazilian National Research Council ...
The synthesis of galacto-oligosaccharides (GOS) by the action of Aspergillus oryzae β-galactosidase free and immobilized on magnetic polysiloxane-polyvinyl alcohol (mPOS-PVA) was studied. A maximum GOS concentration of 26% (w/v) of total sugars was achieved at near 55% lactose conversion from 50%, w/v lactose solution at pH 4.5 and 40 °C. Trisaccharides accounted for more than 81% of the total GOS produced. GOS formation was not considerably affected by pH and temperature. The concentrations of glucose and galactose encountered near maximum GOS concentration greatly inhibited the reactions and reduced GOS yield. GOS formation was not affected by enzyme immobilization in the mPOS-PVA matrix, indicating the absence of diffusional limitations in the enzyme carrier. Furthermore, this water insoluble magnetic derivative was reutilized 10-times and retained about 84% of the initial activity. In addition, the kinetic parameters for various initial lactose concentrations were determined and compared for the free and immobilized enzyme. ; David F. M. Neri gratefully acknowledges support by the Programme Alpan, the European Union Programme of High Level Scholarships for Latin America (Scholarship No. E05D057787BR). Luiz B. Carvalho Jr. is recipient of a scholarship of the Brazilian National Research Council ...
AbstractMYH9 disease is a rare genetic disorder in which there is a mutation in the gene for the non‐muscle myosin heavy chain IIA. It initially causes macrothrombocytopenia followed by other clinical manifestations. When the patient reaches adulthood, he can develop chronic kidney failure. Thus, the risk of suffering a hemorrhage, difficulty in repairing and, infections increases in individuals with this disease. In addition, the use of drugs in these patients should be carefully evaluated. An adult patient sought dental care with a complaint associated with a tooth with advanced dental caries. He had severe thrombocytopenia (7000 platelets/mm3), hearing loss, and chronic kidney failure. The diagnosis of MYH9 disease was confirmed through genotyping. After clinical examination, extraction was planned. Local and systemic procedures were used to prevent hemorrhage, especially postoperatively. Although the patient had an infection at the surgical wound site and no episode of postoperative bleeding, the repair process occurred normally. The purpose of this article is to report the surgical management of a patient with MYH9 disease.
In: Mathysen , D G P , Ringens , P J , Midena , E , Klett , A , Sunaric-Megevand , G , Martinez-Costa , R , Curtin , D , Tassignon , M-J , Aclimandos , W , Creuzot-Garcher , C & Grupcheva , C 2016 , ' Procedural aspects of the organization of the comprehensive European Board of Ophthalmology Diploma examination ' , Journal of educational evaluation for health professions , vol. 13 , 27 . https://doi.org/10.3352/jeehp.2016.13.27
The comprehensive European Board of Ophthalmology Diploma (EBOD) examination is one of 38 European medical specialty examinations. This review aims at disclosing the specific procedures and content of the EBOD examination. It is a descriptive study summarizing the present organization of the EBOD examination. It is the 3rd largest European postgraduate medical assessment after anaesthesiology and cardiology. The master language is English for the Part 1 written test (knowledge test with 52 modified type X multiple-choice questions) (in the past the written test was also available in French and German). Ophthalmology training of minimum 4 years in a full or associated European Union of Medical Specialists (UEMS) member state is a prerequisite. Problem-solving skills are tested in the Part 2 oral assessment, which is a viva of 4 subjects conducted in English with support for native language whenever feasible. The comprehensive EBOD examination is one of the leading examinations organized by UEMS European Boards or Specialist Sections from the point of number of examinees, item banking, and item contents.
International audience ; BACterial Hosts for production of Bioac-tive phenolics from bERRY fruits (BacHBerry) was a 3-year project funded by the Seventh Framework Programme (FP7) of the European Union that ran between November 2013 and October 2016. The overall aim of the project was to establish a sustainable and economically-feasible strategy for the production This article is written by the BacHBerry consortium (www. bachberry.eu) and represents the collective effort of all participating institutions. The authors are therefore listed in alphabetical order. Electronic supplementary material The online version of this article (
International audience ; BACterial Hosts for production of Bioac-tive phenolics from bERRY fruits (BacHBerry) was a 3-year project funded by the Seventh Framework Programme (FP7) of the European Union that ran between November 2013 and October 2016. The overall aim of the project was to establish a sustainable and economically-feasible strategy for the production This article is written by the BacHBerry consortium (www. bachberry.eu) and represents the collective effort of all participating institutions. The authors are therefore listed in alphabetical order. Electronic supplementary material The online version of this article (
International audience ; BACterial Hosts for production of Bioac-tive phenolics from bERRY fruits (BacHBerry) was a 3-year project funded by the Seventh Framework Programme (FP7) of the European Union that ran between November 2013 and October 2016. The overall aim of the project was to establish a sustainable and economically-feasible strategy for the production This article is written by the BacHBerry consortium (www. bachberry.eu) and represents the collective effort of all participating institutions. The authors are therefore listed in alphabetical order. Electronic supplementary material The online version of this article (
International audience ; BACterial Hosts for production of Bioac-tive phenolics from bERRY fruits (BacHBerry) was a 3-year project funded by the Seventh Framework Programme (FP7) of the European Union that ran between November 2013 and October 2016. The overall aim of the project was to establish a sustainable and economically-feasible strategy for the production This article is written by the BacHBerry consortium (www. bachberry.eu) and represents the collective effort of all participating institutions. The authors are therefore listed in alphabetical order. Electronic supplementary material The online version of this article (
In: Dudnik , A , Almeida , A F , Andrade , R , Avila , B , Banados , P , Barbay , D , Bassard , J-E , Benkoulouche , M , Bott , M , Braga , A , Breitel , D , Brennan , R , Bulteau , L , Chanforan , C , Costa , I , Costa , R S , Doostmohammadi , M , Faria , N , Feng , C , Fernandes , A , Ferreira , P , Ferro , R , Foito , A , Freitag , S , Garcia , G , Gaspar , P , Godinho-Pereira , J , Hamberger , B , Hartmann , A , Heider , H , Jardim , C , Julien-Laferriere , A , Kallscheuer , N , Kerbe , W , Kuipers , O P , Li , S , Love , N , Marchetti-Spaccamela , A , Marienhagen , J , Martin , C , Mary , A , Mazurek , V , Meinhart , C , Sevillano , D M , Menezes , R , Naesby , M , Norholm , M H H , Okkels , F T , Ottens , M & Solopova , A 2018 , ' BacHBerry : BACterial Hosts for production of Bioactive phenolics from bERRY fruits ' , Phytochemistry reviews , vol. 17 , no. 2 , pp. 291-326 . https://doi.org/10.1007/s11101-017-9532-2 ; ISSN:1568-7767
BACterial Hosts for production of Bioactive phenolics from bERRY fruits (BacHBerry) was a 3-year project funded by the Seventh Framework Programme (FP7) of the European Union that ran between November 2013 and October 2016. The overall aim of the project was to establish a sustainable and economically-feasible strategy for the production of novel high-value phenolic compounds isolated from berry fruits using bacterial platforms. The project aimed at covering all stages of the discovery and pre-commercialization process, including berry collection, screening and characterization of their bioactive components, identification and functional characterization of the corresponding biosynthetic pathways, and construction of Gram-positive bacterial cell factories producing phenolic compounds. Further activities included optimization of polyphenol extraction methods from bacterial cultures, scale-up of production by fermentation up to pilot scale, as well as societal and economic analyses of the processes. This review article summarizes some of the key findings obtained throughout the duration of the project.
In: Dudnik , A , Almeida , A F , Andrade , R , Avila , B , Bañados , P , Barbay , D , Bassard , J E , Benkoulouche , M , Bott , M , Braga , A , Breitel , D , Brennan , R , Bulteau , L , Chanforan , C , Costa , I , Costa , R S , Doostmohammadi , M , Faria , N , Feng , C , Fernandes , A , Ferreira , P , Ferro , R , Foito , A , Freitag , S , Garcia , G , Gaspar , P , Godinho-Pereira , J , Hamberger , B , Hartmann , A , Heider , H , Jardim , C , Julien-Laferriere , A , Kallscheuer , N , Kerbe , W , Kuipers , O P , Li , S , Love , N , Marchetti-Spaccamela , A , Marienhagen , J , Martin , C , Mary , A , Mazurek , V , Meinhart , C , Sevillano , D M , Menezes , R , Naesby , M , Nørholm , M H H , Okkels , F T , Oliveira , J , Ottens , M , Parrot , D , Pei , L , Rocha , I , Rosado-Ramos , R , Rousseau , C , Sagot , M F , dos Santos , C N , Schmidt , M , Shelenga , T , Shepherd , L , Silva , A R , da Silva , M H , Simon , O , Stahlhut , S G , Solopova , A , Sorokin , A , Stewart , D , Stougie , L , Su , S , Thole , V , Tikhonova , O , Trick , M , Vain , P , Veríssimo , A , Vila-Santa , A , Vinga , S , Vogt , M , Wang , L , Wang , L , Wei , W , Youssef , S , Neves , A R & Forster , J 2018 , ' BacHBerry : BACterial Hosts for production of Bioactive phenolics from bERRY fruits ' , Phytochemistry Reviews , vol. 17 , no. 2 , pp. 291-326 . https://doi.org/10.1007/s11101-017-9532-2
BACterial Hosts for production of Bioactive phenolics from bERRY fruits (BacHBerry) was a 3-year project funded by the Seventh Framework Programme (FP7) of the European Union that ran between November 2013 and October 2016. The overall aim of the project was to establish a sustainable and economically-feasible strategy for the production of novel high-value phenolic compounds isolated from berry fruits using bacterial platforms. The project aimed at covering all stages of the discovery and pre-commercialization process, including berry collection, screening and characterization of their bioactive components, identification and functional characterization of the corresponding biosynthetic pathways, and construction of Gram-positive bacterial cell factories producing phenolic compounds. Further activities included optimization of polyphenol extraction methods from bacterial cultures, scale-up of production by fermentation up to pilot scale, as well as societal and economic analyses of the processes. This review article summarizes some of the key findings obtained throughout the duration of the project.
BACterial Hosts for production of Bioactive phenolics from bERRY fruits (BacHBerry) was a 3-year project funded by the Seventh Framework Programme (FP7) of the European Union that ran between November 2013 and October 2016. The overall aim of the project was to establish a sustainable and economically-feasible strategy for the production of novel high-value phenolic compounds isolated from berry fruits using bacterial platforms. The project aimed at covering all stages of the discovery and pre-commercialization process, including berry collection, screening and characterization of their bioactive components, identification and functional characterization of the corresponding biosynthetic pathways, and construction of Gram-positive bacterial cell factories producing phenolic compounds. Further activities included optimization of polyphenol extraction methods from bacterial cultures, scale-up of production by fermentation up to pilot scale, as well as societal and economic analyses of the processes. This review article summarizes some of the key findings obtained throughout the duration of the project.