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Natural processes contradictive to the common organic (sedimentary and migrational) theory of oil formation were concluded to take place based on the study of the hydrocarbons-biomarkers distribution regularities at the molecular level in the dispersed organic matter of rocks, petroleum and kerogen thermolysis products.Here specific examples of the generation of oil (concentrated organic matter) directly in the reservoir, i.e. reduced scheme of naphthydogenesis, excluding the process of migration were presented. At the same time, these examples do not exclude the migration of organic matter from the «oil source strata», but are an exception that confirms the general rule. It was shown that the study of organic matter, organic carbon (TOC), chloroform-soluble bitumen, pyrolysis "Rock-Eval", etc., necessarily supporting the conclusions with data from a detailed study of the hydrocarbon composition at the molecular level.

Until now the issue of party and political work among the Northern Fleet submarines brigade has never been a key topic for research of the national historians and area studies specialists. Our study aims at comprehensive examination of party and political work conducted on the Northern Fleet submarines in 1941–1945. In order to achieve this goal, we had to define the subject-matter and stages of party and political work, to identify its goals and character, to show the instruments for its execution, specifying its merits and flaws. We came to a conclusion that the political work among the crew members of the Northern Fleet submarines was conducted inconsistently during the Great Patriotic War. At the first stage of work with military personnel and during preparation of political information the political workers approached the problem as a mere formality. They did not take into account the age, education, positions of the listeners. Such approach and methods did not resonate with the military personnel. The political department used standard means of agitation such as lectures, discussions, reading newspapers. Moreover, the activity reports were based on formalist principles with unpalatable facts kept quiet. After 1943 the party and political work would become more systematic. Differentiation, awareness and sequence of operations could be observed in the actions of political department. The political work would be vertically arranged and would interconnect the Northern Fleet staff, brigade headquarters, brigade divisions, submarines. The means of campaigning and propaganda that had been already in place would be supplemented by newspapers, radio, political studies, excursions, photo albums.

The purpose of this article is a historical and anthropological examination of the phenomenon of fear, the methods of its manifestation and overcoming in the shadow of war. The authors refer to battlefield and postwar diaries from 1943–1946 of G. I. Sennikov, a submarine sailor of the Northern Fleet, marine electrician of "М-107" and "М-119". The authors use methods of historiographical and mythopoetic analysis, and the biographic method. In Sennikov's battlefield diary, the authors observe the sailor's analytical approach to the problem of fear: his story is not just a documentation and detailed description of the physical signs of horror, but also a classification of the types of fear, exploration of different aspects of a person's fear at war, and an attempt to get an insight into the essence of this phenomenon. The research reveals that the young sailor perceived the war as a death-defying admission and initiation at the ultimate threshold and is described with the help of archetypical figures: a monster ship, a coffin boat, the sea, etc. The young man who found himself in the extremely harsh wartime conditions identifies such characteristic features of a submarine sailor's psychology as sailor fatalism, a certain superstitious religiousness (belief in signs, dreams, "marked" spaces and taboos, amulets, and taboo systems). In his diaries, G. I. Sennikov does not only identify superstitious religiousness and ritual activities as the most efficient weapon against fear, but also creativity, laughter, games, and, most significantly, personal values, the authority of commanding officers, and conviction that Soviet submarine sailors fight for the right cause, on the side of the good, saving the world from the horrors of fascism.

Filoviruses, such as Ebola virus and Marburg virus, are of significant human health concern. From 2013 to 2016, Ebola virus caused 11,323 fatalities in Western Africa. Since 2018, two Ebola virus disease outbreaks in the Democratic Republic of the Congo resulted in 2354 fatalities. Although there is progress in medical countermeasure (MCM) development (in particular, vaccines and antibody-based therapeutics), the need for efficacious small-molecule therapeutics remains unmet. Here we describe a novel high-throughput screening assay to identify inhibitors of Ebola virus VP40 matrix protein association with viral particle assembly sites on the interior of the host cell plasma membrane. Using this assay, we screened nearly 3000 small molecules and identified several molecules with the desired inhibitory properties. In secondary assays, one identified compound, sangivamycin, inhibited not only Ebola viral infectivity but also that of other viruses. This finding indicates that it is possible for this new VP40-based screening method to identify highly potent MCMs against Ebola virus and its relatives.

Biosafety, biosecurity, logistical, political, and technical considerations can delay or prevent the wide dissemination of source material containing viable virus from the geographic origin of an outbreak to laboratories involved in developing medical countermeasures (MCMs). However, once virus genome sequence information is available from clinical samples, reverse-genetics systems can be used to generate virus stocks de novo to initiate MCM development. In this study, we developed a reverse-genetics system for natural isolates of Ebola virus (EBOV) variants Makona, Tumba, and Ituri, which have been challenging to obtain. These systems were generated starting solely with in silico genome sequence information and have been used successfully to produce recombinant stocks of each of the viruses for use in MCM testing. The antiviral activity of MCMs targeting viral entry varied depending on the recombinant virus isolate used. Collectively, selecting and synthetically engineering emerging EBOV variants and demonstrating their efficacy against available MCMs will be crucial for answering pressing public health and biosecurity concerns during Ebola disease (EBOD) outbreaks.

Biosafety, biosecurity, logistical, political, and technical considerations can delay or prevent the wide dissemination of source material containing viable virus from the geographic origin of an outbreak to laboratories involved in developing medical countermeasures (MCMs). However, once virus genome sequence information is available from clinical samples, reverse-genetics systems can be used to generate virus stocks de novo to initiate MCM development. In this study, we developed a reverse-genetics system for natural isolates of Ebola virus (EBOV) variants Makona, Tumba, and Ituri, which have been challenging to obtain. These systems were generated starting solely with in silico genome sequence information and have been used successfully to produce recombinant stocks of each of the viruses for use in MCM testing. The antiviral activity of MCMs targeting viral entry varied depending on the recombinant virus isolate used. Collectively, selecting and synthetically engineering emerging EBOV variants and demonstrating their efficacy against available MCMs will be crucial for answering pressing public health and biosecurity concerns during Ebola disease (EBOD) outbreaks.

Ebolaviruses cause an often rapidly fatal syndrome known as Ebola virus disease (EVD), with average case fatality rates of ~50%. There is no licensed vaccine or treatment for EVD, underscoring the urgent need to develop new anti-ebolavirus agents, especially in the face of an ongoing outbreak in the Democratic Republic of the Congo and the largest ever outbreak in Western Africa in 2013–2016. Lectins have been investigated as potential antiviral agents as they bind glycans present on viral surface glycoproteins, but clinical use of them has been slowed by concerns regarding their mitogenicity, i.e. ability to cause immune cell proliferation. We previously engineered a banana lectin (BanLec), a carbohydrate-binding protein, such that it retained antiviral activity but lost mitogenicity by mutating a single amino acid, yielding H84T BanLec (H84T). H84T shows activity against viruses containing high-mannose N-glycans, including influenza A and B, HIV-1 and -2, and hepatitis C virus. Since ebolavirus surface glycoproteins also contain many high-mannose N-glycans, we assessed whether H84T could inhibit ebolavirus replication. H84T inhibited Ebola virus (EBOV) replication in cell cultures. In cells, H84T inhibited both virus-like particle (VLP) entry and transcription/replication of the EBOV mini-genome at high micromolar concentrations, while inhibiting infection by transcription- and replication-competent VLPs, which measures the full viral life cycle, in the low micromolar range. H84T did not inhibit assembly, budding, or release of VLPs. These findings suggest that H84T may exert its anti-ebolavirus effect(s) by blocking both entry and transcription/replication. In a mouse model, H84T partially (maximally, ~50–80%) protected mice from an otherwise lethal mouse-adapted EBOV infection. Interestingly, a single dose of H84T pre-exposure to EBOV protected ~80% of mice. Thus, H84T shows promise as a new anti-ebolavirus agent with potential to be used in combination with vaccination or other agents in a prophylactic or ...

Frontmatter -- Contents -- Digital Economy in the 21 Century: An Introduction to the Institutional Approach -- Part I: The Scientific Concept of the Digital Economy in the 21st Century -- 1 Digital Economy as a Modern Type of Economic System -- 2 "Digitalization"- Overcoming Institutional Barriers -- 3 Development of the Information Technologies Sector in Latvia under Globalization -- 4 The Principles of Functioning and Priorities of Development of the Digital Economy -- 5 Classification of Breakthrough Digital Technologies and the Perspectives of Their Application in Economy -- Part II: The Process of Digital Economy Institutionalization in the 21st Century -- 6 The Essence and Logic of the Process of Sectorial Markets' Digital Transformation -- 7 The Current Tendencies of Economy Digitalization in Developed and Developing Countries -- 8 The Main Stages of the Digital Modernization of Economy -- 9 Implementation of Cluster Initiatives in the Digital Sphere as a Tool of Digital Entrepreneurship's Institutionalization -- 10 Institutions of Support for Digital Entrepreneurship: Special Economic Zones, Innovative Networks and Technological Parks -- Part III: Meso-Level Institutions of the Digital Economy in the 21st Century -- 11 Digitalization of Regional Economy: Problems and Perspectives -- 12 The Institutional Model of the Digital Economy Creation in a Modern Region -- 13 Managing a Modern Region Based on Digital Technologies -- Part IV: Macro-Level Institutions of the Digital Economy in the 21st Century -- 14 State Institutional Regulation of Economy Digital Modernization -- 15 The Role of Financial Institutions in Supporting the Digital Economy -- 16 Digital Economy of the 21st Century: A View from the Positions of Developed and Developing Countries -- Part V: The Global Institutions of the Digital Economy in the 21st Century -- 17 International Trade in the Digital Sphere: Barriers and Prospects for Development -- 18 The Existing and Perspective International Institutions for Supporting Digital Transformation of Economy -- 19 The Scientific and Methodological Approach to Provision and Evaluation of the Digital Economy's Global Competitiveness -- 20 The Strategy of Optimal Development of the Digital Economy: A View from the Positions of Game Theory -- 21 The Institutional Model of Well-Balanced and Sustainable Digital Economy -- 22 The Institutional Mechanism of Managing the Digital Economy's Development -- Part VI: Case Studies of Institutions of the Digital Economy in the 21st Century -- 23 Problems and Prospects of Economic Cooperation Between Russia and Mexico -- 24 Innovative Critical Success Factors for Public – Private Partnerships (PPP) in Infrastructure Projects of Developing Countries. A Case of Zambia -- 25 Prediction Mechanism of the Territorial Socio-Economic Processes in Formation of the Information Systems -- 26 Specific Economic Security Regulations in the Context of Pathological Crises of Digital Transformation of Agricultural Organizations -- Conclusion: Institutional Perspectives of the Digital Economy's Development in the 21st Century -- List of Figures -- List of Tables -- Index

The paper deals with possible contribution of botanical data of the Institute for Biological Problems of Cryolithozone SA RAS to such international databases (ID) as GBIF and Arctic Vegetation Archive. Collected data on flora and vegetation, as well as herbarium and botanical garden collections are characterized, and their promptness for publication in ID is evaluated. Non-digital data are possible to be published as metadata.