Protein Recovery and Quality of Alfalfa Extracts Obtained by Acid Precipitation and Fermentation
In: BITEB-D-22-00598
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In: BITEB-D-22-00598
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In: Environmental and resource economics, Band 84, Heft 1, S. 37-63
ISSN: 1573-1502
In: Tsochatzis , E D , Lopes , J A , Gika , H , Dalsgaard , T K & Theodoridis , G 2021 , ' A fast SALLE GC–MS/MS multi-analyte method for the determination of 75 food packaging substances in food simulants ' , Food Chemistry , vol. 361 , 129998 . https://doi.org/10.1016/j.foodchem.2021.129998
A simple and fast method was developed for the simultaneous determination of 75 plastic food contact material (FCM) in liquid food simulants, at levels of a few ng g −1 . The method employs an optimised salt-assisted liquid–liquid extraction for all EU-regulated ethanol/H 2 O food simulants, in the presence of 10% NaCl (simulants A and C) or 5% NaCl (simulant D1), and dichloromethane as the extracting solvent. Gas chromatography with triple-quad MS operating in multiple reaction monitoring acquisition was used, applying isotope dilution with selected deuterated compounds. Adequate sensitivity was demonstrated for all analytes. The results also showed sufficient accuracy for the majority of substances, with recoveries of 70–120% and repeatability (expressed as relative standard deviations, RSDs) smaller than 15%. The method was applied to the analysis of FCM multilayer items after undergoing migration testing according to the specifications of the current EU legislation in force.
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Antimicrobial resistance is a global public health concern, and resistance genes in Salmonella, especially those located on mobile genetic elements, are part of the problem. This study used phenotypic and genomic methods to identify antimicrobial resistance and resistance genes, as well as the plasmids that bear them, in Salmonella isolates obtained from poultry in Nigeria. Seventy-four isolates were tested for susceptibility to eleven commonly used antimicrobials. Plasmid reconstruction and identification of resistance and virulence genes were performed with a draft genome using in silico approaches in parallel with plasmid extraction. Phenotypic resistance to ciprofloxacin (50.0%), gentamicin (48.6%), nalidixic acid (79.7%), sulphonamides (71.6%) and tetracycline (59.5%) was the most observed. Antibiotic resistance genes (ARGs) detected in genomes corresponded well with these observations. Commonly observed ARGs included sul1, sul2, sul3, tet (A), tet (M), qnrS1, qnrB19 and a variety of aminoglycoside-modifying genes, in addition to point mutations in the gyrA and parC genes. Multiple ARGs were predicted to be located on IncN and IncQ1 plasmids of S. Schwarzengrund and S. Muenster, and most qnrB19 genes were carried by Col (pHAD28) plasmids. Seventy-two percent (19/24) of S. Kentucky strains carried multidrug ARGs located in two distinct variants of Salmonella genomic island I. The majority of strains carried full SPI-1 and SPI-2 islands, suggesting full virulence potential ; This work was part-supported by an African Research Leader Award to INO from the UK Medical Research Council (MRC) and the UK Department for International Development (DFID) under the MRC/DFID Concordat agreement that is also part of the EDCTP2 programme supported by the European Union. VG acknowledges Consellería de Cultura, Educación e Ordenación Universitaria, Xunta de Galicia for her post-doctoral grant (Grant Number ED481B-2018/018) ; SI
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In: Waste management: international journal of integrated waste management, science and technology, Band 164, S. 191-199
ISSN: 1879-2456
eGovernment and autoritative public data in Denmark are still mainly focused on the landside of Danmark. But the need of eloborating the coordinating effort of administration at sea is recognised and the development of maritime eGovernment is happening within the next years. This paper discusses the data involved in this administration and focuses on the need for understanding the function and hence value of data. Some maritime data are dealing with accurate placements of objects, and as a tricky part some are dealing with floating placement. Other data are handling non-visible areas in terms of zoning, e.g. planning and interest areas and finally some data are representation of legislation, whether it is rights or restrictions. These different data are not always usable in the same manner. This paper investigates the correlation between spatial data and legislation. Experience from many years of land administration can be used as part of this discussion as well as the conclusions from this paper can be discussion as input to the ongoing development on land.
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eGovernment and autoritative public data in Denmark are still mainly focused on the landside of Danmark. But the need of eloborating the coordinating effort of administration at sea is recognised and the development of maritime eGovernment is happening within the next years. This paper discusses the data involved in this administration and focuses on the need for understanding the function and hence value of data. Some maritime data are dealing with accurate placements of objects, and as a tricky part some are dealing with floating placement. Other data are handling non-visible areas in terms of zoning, e.g. planning and interest areas and finally some data are representation of legislation, whether it is rights or restrictions. These different data are not always usable in the same manner. This paper investigates the correlation between spatial data and legislation. Experience from many years of land administration can be used as part of this discussion as well as the conclusions from this paper can be discussion as input to the ongoing development on land.
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WOS: 000380030700003 ; The Massive Open Online Course (MOOC) movement is the latest "big thing" in Open and Distance Learning (ODL). MOOCs offer both opportunities and threats that are extensively discussed in the literature, including the potential of opening up education for all at a global scale. On the other hand, MOOCs challenge traditional pedagogy and raise important questions about the future of campus-based education. However, in discussing these opportunities and threats the majority of the literature tends to focus on the origin of the MOOC movement in the United States (US). The specific context of Europe with its diversity of languages, cultural environments, educational policies, and regulatory frameworks differs substantially from the US context. Accordingly, this article offers a European perspective on MOOCs in order to better understand major differences in threats and opportunities across countries and continents, including the use and reuse of MOOCs for regional or global use, via European or non-European platforms. In the context of the EU funded HOME project (Higher education Online: MOOCs the European way), a research initiative was undertaken to identify opportunities and threats of the MOOC movement for European higher education institutions. Three sources of data were gathered and analysed. Opportunities and threats were categorized into two levels. The macro level comprises issues related to the higher education system, European context, historical period, and institutional concerns. The micro level covers aspects related to faculty, teachers, and courses, thus to the operational level. The main opportunities discovered were: the ECTS as a robust system for formal recognition of accomplishments in MOOCs; the trend for institutional collaboration, stimulated by EU-funded programs; and the many innovative and alternative pedagogical models used in MOOCs published in Europe. The main threats mentioned were: implementation problems of the ECTS, difficulties in bridging non/informal and formal education; and too much regulation, hindering experimentation and innovation. ; European Union under European Commission, DG EAC, under the Lifelong Learning Programme [543516-LLP-1-2013-1-NL-KA3-KA3NW] ; This research was conducted in the framework of the European Union-funded project HOME Higher education Online: MOOCs the European way (Ref. 543516-LLP-1-2013-1-NL-KA3-KA3NW) under the support of the European Commission, DG EAC, under the Lifelong Learning Programme. The authors wish also to extend their acknowledgements to all the contributors and participants to the conference "Mapping the European MOOC Territory" held in Porto on 27th November, 2014, for their contribution to this study.
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In: Scientific African, Band 7, S. e00289
ISSN: 2468-2276
The Massive Open Online Course (MOOC) movement is the latest "big thing" in Open and Distance Learning (ODL). MOOCs offer both opportunities and threats that are extensively discussed in the literature, including the potential of opening up education for all at a global scale. On the other hand, MOOCs challenge traditional pedagogy and raise important questions about the future of campus-based education. However, in discussing these opportunities and threats the majority of the literature tends to focus on the origin of the MOOC movement in the United States (US). The specific context of Europe with its diversity of languages, cultural environments, educational policies, and regulatory frameworks differs substantially from the US context. Accordingly, this article offers a European perspective on MOOCs in order to better understand major differences in threats and opportunities across countries and continents, including the use and reuse of MOOCs for regional or global use, via European or non-European platforms. In the context of the EU funded HOME project (Higher education Online: MOOCs the European way), a research initiative was undertaken to identify opportunities and threats of the MOOC movement for European higher education institutions. Three sources of data were gathered and analysed. Opportunities and threats were categorized into two levels. The macro level comprises issues related to the higher education system, European context, historical period, and institutional concerns. The micro level covers aspects related to faculty, teachers, and courses, thus to the operational level. The main opportunities discovered were: the ECTS as a robust system for formal recognition of accomplishments in MOOCs; the trend for institutional collaboration, stimulated by EU-funded programs; and the many innovative and alternative pedagogical models used in MOOCs published in Europe. The main threats mentioned were: implementation problems of the ECTS, difficulties in bridging non/informal and formal education; and too ...
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In: Schuwer , R , Gil-Jaurena , I , Hakan Aydin , C , Costello , E , Dalsgaard , C , Brown , M , Jansen , D & Teixeira , A 2015 , ' Opportunities and threats of the MOOC movement for higher education: the European perspective ' , International Review of Research in Open and Distributed Learning , vol. 16 , no. 6 , pp. 20-38 . https://doi.org/10.19173/irrodl.v16i6.2153
The Massive Open Online Course (MOOC) movement is the latest 'big thing' in Open and Distance Learning (ODL) which threatens to transform Higher Education. Both opportunities and threats are extensively discussed in literature, comprising issues on opening up education for the whole world, pedagogy and online versus campus education. Most of the literature focus on the origin of the MOOC movement in the US. The specific context of Europe with on the one hand autonomous countries and educational systems and on the other hand cross-border cooperation and regulations through the European Union differs from the US context. This specific context can influence the way in which the MOOC movement affect education in Europe, both reusing MOOCs from other continents (US) as publishing MOOCs, on a European platform or outside of Europe. In the context of the EU funded HOME project, a research was conducted to identify opportunities and threats of the MOOC movement on the European institutions of higher education. Three sources of data were gathered and analysed. Opportunities and threats were categorized in two levels. The macro level comprises issues related to the higher education system, European context, historical period and institutional level. The micro level covers aspects related to faculty, professors and courses, thus to the operational level. The main opportunities mentioned were the ECTS system as being a sound base for formal recognition of accomplishments in MOOCs, the tendency to cooperate between institutions, stimulated by EU funded programs and the many innovative pedagogical models used in MOOCs published in Europe. The main threats mentioned were a lacking implementation of the ECTS system, hindering bridging non/formal and formal education and too much regulation, hindering experimenting and innovation.
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The Massive Open Online Course (MOOC) movement is the latest "big thing" in Open and Distance Learning (ODL). MOOCs offer both opportunities and threats that are extensively discussed in the literature, including the potential of opening up education for all at a global scale. On the other hand, MOOCs challenge traditional pedagogy and raise important questions about the future of campus-based education. However, in discussing these opportunities and threats the majority of the literature tends to focus on the origin of the MOOC movement in the United States (US). The specific context of Europe with its diversity of languages, cultural environments, educational policies, and regulatory frameworks differs substantially from the US context. Accordingly, this article offers a European perspective on MOOCs in order to better understand major differences in threats and opportunities across countries and continents, including the use and reuse of MOOCs for regional or global use, via European or non-European platforms. In the context of the EU funded HOME project (Higher education Online: MOOCs the European way), a research initiative was undertaken to identify opportunities and threats of the MOOC movement for European higher education institutions. Three sources of data were gathered and analysed. Opportunities and threats were categorized into two levels. The macro level comprises issues related to the higher education system, European context, historical period, and institutional concerns. The micro level covers aspects related to faculty, teachers, and courses, thus to the operational level. The main opportunities discovered were: the ECTS as a robust system for formal recognition of accomplishments in MOOCs; the trend for institutional collaboration, stimulated by EU-funded programs; and the many innovative and alternative pedagogical models used in MOOCs published in Europe. The main threats mentioned were: implementation problems of the ECTS, difficulties in bridging non/informal and formal education; and too ...
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The marine bacterium Photobacterium damselae subsp. damselae is a pathogen for a variety of marine animals, as well as for humans, and is nowadays considered an emerging pathogen for fish of importance in marine aquaculture. Recent studies have suggested that outbreaks in fish farms are caused by multiclonal populations of this subspecies that exist in the environment. Here, we report the study of a collection of 31 strains isolated during the course of disease outbreaks in marine rainbow trout farms in Denmark in 1994, 1995, and 2006, respectively. A phylogenetic analysis based on the toxR gene sequence, and the screening of virulence-related genes uncovered a high genetic heterogeneity, even among strains isolated from the same fish farm at the same time. Moreover, comparative analysis of the whole genome sequences of four selected strains revealed a large number of differentially occurring genes, which included virulence genes, pPHDD1 plasmid, polysaccharide synthesis gene clusters, CRISPR-Cas systems and putative new mobile genetic elements. This study provides sound evidence that P. damselae subsp. damselae outbreaks in Danish rainbow trout farms were caused by multiclonal populations and that horizontal gene transfer constitutes a strong driving force in the generation of intraspecific diversity in this pathogen ; This work has been supported by grant AGL2016-79738-R (AEI/FEDER, EU) from the State Agency for Research (AEI) of Spain, and co-funded by the FEDER Programme from the European Union. The support of Xunta de Galicia (Spain) with grant GRC-2014/007 is also acknowledged. MT thanks the Brazilian Ministry of Education and CAPES (Coordenaçao de Aperfeiçoamento de Pessoal de Nível Superior) for a predoctoral fellowship. XM thanks Xunta de Galicia for a predoctoral fellowship ; SI
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In: Bjørk , R , Dalsgaard , P , Dimke , H , Jauffred , L , Møller , N M , Niss , K , Pedersen , B P , Sørensen , T J & Vallgårda , K A A 2020 , ' Unge forskere: Fri forskning er vigtigt – også i krisetider ' , Altinget .
DEBAT: Der er behov for at skabe oversigt over og mobilisere den danske forskningsverden i krisesituationer. Men vi vil advare imod direkte politisk styring, som tidligere forskningsminister Søren Pind efterspørger, skriver en række unge forskere.
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