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The New Zealand government has been praised for heeding scientific advice in response to the COVID-19 pandemic, but when it comes to environmental protections the scientific advice seems to be negotiable. Freshwaters have been in decline for decades, despite clear science on limits needed to protect them. There are many examples of 'shifting baselines', where limits have been progressively weakened through agency regulatory capture and political expediency.

The nutrient dynamics and biological structure of shallow non-stratified lakes differ markedly from that of deep and stratified lakes: for example, the return of nutrients lost through sedimentation is faster and the potential importance of fish and submerged macrophytes as food-web regulators is greater. In addition shallow lakes are more easily influenced by fluctuations in the physical environment caused by wind disturbance, temperature change, etc. Although shallow lakes are often the most common lake type in lowland countries, less attention has been paid to them than to deep stratified lakes and few comparisons have been made between shallow freshwater and brackish lakes. The volume is divided into five main themes, each introduced by an invited speaker: Nutrient dynamics with special emphasis on sediment water interactions and changes in loading (Professor Lambertus Lyklema); Biological structure and trophic interaction (Professor Stephen T. Threlkeld); Submerged macrophytes: dynamics and role in the lake ecosystems (Ass. Professor Kaj Sand Jensen); Comparison of freshwater and brackish lake ecosystems (Professor Brian Moss); Modelling the impact of nutrients, the biological structure and the recovery process (Dr Marten Scheffer)

There is still a different perspective on the fishery aspect of the importance of diversifying the fish farming business in freshwater, causing a low quantity of aquaculture entrepreneurs as well as the diversity of fish cultivation businesses in freshwater. Therefore, a reference basis is needed through a study on the perspective of these stakeholders to determine an appropriate cultivation business strategy to be implemented. The study used interview methods and questionnaires for 40 stakeholder respondents in the field of fisheries and analyzed with Analysis Hierarchy Process (AHP). The results show that land suitability, supportive laws and regulations, market demand, public perception/perspective, and adequate availability of human resources are the main contributing factors in the expansion of the freshwater fish farming business on Sibundong river. The actors expected by the community in the expansion of fish aquaculture business are Bappeda, Freshwater Seed Hall, Marine and Fisheries Affairs and Government of Central Tapanuli, and Matauli College of Fisheries and Marine Science. All of them can be achieved by coordinating between relevant agencies intensively, conducting training/making pilot pools of freshwater cultivation, improving the distribution channels of freshwater aquaculture fisheries, conducting multi-species cultivation, and conducting environmentally-minded cultivation.

This work was supported by the Latvian Council of Science , project NN-CARMA, project No. lzp-2018/1-0194. ; Background: This work studied how the exposure to an unusual substrate forced a change in microbial populations during anaerobic fermentation of crude glycerol, a by-product of biodiesel production, with freshwater sediment used as an inoculum. Results: The microbial associations almost completely (99.9%) utilized the glycerol contained in crude glycerol 6 g L−1 within four days, releasing gases, organic acids (acetic, butyric) and alcohols (ethanol, n-butanol) under anaerobic conditions. In comparison with control medium without glycerol, adding crude glycerol to the medium increased the amount of ethanol and n-butanol production and it was not significantly affected by incubation temperature (28 °C or 37 °C), nor incubation time (4 or 8 d), but it resulted in reduced amount of butyric acid. Higher volume of gas was produced at 37 °C despite the fact that the overall bacterial count was smaller than the one measured at 20 °C. Main microbial phyla of the inoculum were Actinobacteria, Proteobacteria and Firmicutes. During fermentation, significant changes were observed and Firmicutes, especially Clostridium spp., began to dominate, and the number of Actinobacteria and Gammaproteobacteria decreased accordingly. Concentration of Archaea decreased, especially in medium with crude glycerol. These changes were confirmed both by culturing and culture-independent (concentration of 16S rDNA) methods. Conclusions: Crude glycerol led to the adaptation of freshwater sediment microbial populations to this substrate. Changes of microbial community were a result of a community adaptation to a new source of carbon. How to cite: Paiders M, Nikolajeva V, Makarenkova G, et al. Changes in freshwater sediment microbial populations during fermentation of crude glycerol. ; Latvian Council of Science lzp-2018/1-0194; Institute of Solid State Physics, University of Latvia as the Center of Excellence has received funding from the European Union's Horizon 2020 Framework Programme H2020-WIDESPREAD-01-2016-2017-TeamingPhase2 under grant agreement No. 739508, project CAMART²

The widespread salinisation of freshwater ecosystems poses a major threat to the biodiversity, functioning, and services that they provide. Human activities promote freshwater salinisation through multiple drivers (e.g., agriculture, resource extraction, urbanisation) that are amplified by climate change. Due to its complexity, we are still far from fully understanding the ecological and evolutionary consequences of freshwater salinisation. Here, we assess current research gaps and present a research agenda to guide future studies. We identified different gaps in taxonomic groups, levels of biological organisation, and geographic regions. We suggest focusing on global- and landscape-scale processes, functional approaches, genetic and molecular levels, and eco-evolutionary dynamics as key future avenues to predict the consequences of freshwater salinisation for ecosystems and human societies. ; This work is part of the AQUACOSM and AQUACOSM-plus projects that have received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement Nos 731065 and 871081. D.C.M., M.B., E.J., R.P., S.A.B., M.G., Z.H., S.L., J.C.N., M.R., and C.F.V. were supported by the H2020 EU-funded project AQUACOSM-plus (no. 871081). D.C.M., M.B., S.A.B., and J.C.N. were supported by the H2020 EU-funded project AQUACOSM (no. 731065). M.B. and E.J. were supported by the TÜBITAK program BIDEB2232 (project 118C250). M.B. and S.B. were supported by the H2020 EU-funded project PONDERFUL (no. 869296). Z.H. and C.F.V. were supported by the NKFIH-471-3/2021 project. Z.H. was supported by the János Bolyai Research Scholarship of the Hungarian Academy of Sciences. P.U-C. was supported by the Swedish Research Council Formas (grant: 2020-01825). M.M. was supported by the Grant RyC2016-19348 funded by MCIN/AEI/10.13039/501100011033 and by ESF 'Investing in your future'

This book is based on a workshop on biogeography of freshwater algae held during the Fifth International Phycological Congress in China 1994. A group of outstanding specialists covering widely different approaches to the subject have been brought together, and this collection of their contributions forms a unique volume: there is no other book on the subject. It thus fills an evident gap in the phycological literature, and will be of major interest to researchers and teachers within phycology, limnology, and evolutionary biology. However, it may also be useful in courses for advanced students

Piria, Marina/0000-0001-6324-9282; Simonovic, Predrag/0000-0002-4819-4962; ; WOS: 000418927100009 ; Fish introductions, particularly in areas of high biological diversity and endemism, represent a major threat for biodiversity. In the Balkan Peninsula, 60 fish species have been introduced to date, of which 36 have become naturalized in inland waters. Since the Balkans are one of the world's 35 biodiversity hot spots, this large presence of alien fish species poses a serious threat for the stability of freshwater ecosystems and the survival of the native ichthyofauna and of aquatic biodiversity in general. The motivation for the introductions, and the historical timeline, varies among the Balkan states. Despite recent attempts to implement and align legislation aimed at preventing the introduction of potentially invasive species, and the implementation of rigorous controls of introductions and increased protection of open waters, the majority of current introductions remain intentional, primarily via aquaculture. This review article provides a historical overview of freshwater fish introductions, the motivation behind them and the current distribution of alien freshwater fishes in the Balkans. The ecological implications and future perspectives concerning alien fish species in the region are also discussed. ; University of Zagreb, Croatia; Ministry of Education, Science and Technological Development of Serbia [173025] ; University of Zagreb, Croatia; Ministry of Education, Science and Technological Development of Serbia, Grant/Award Number: 173025

Using a new fossil-calibrated mitogenome-based approach, we identified macroevolutionary shifts in mitochondrial gene order among the freshwater mussels (Unionoidea). We show that the early Mesozoic divergence of the two Unionoidea clades, Margaritiferidae and Unionidae, was accompanied by a synchronous split in the gene arrangement in the female mitogenome (i.e., gene orders MF1 and UF1). Our results suggest that this macroevolutionary jump was completed within a relatively short time interval (95% HPD 201–226 Ma) that coincided with the Triassic–Jurassic mass extinction. Both gene orders have persisted within these clades for ~200 Ma. The monophyly of the so-called "problematic" Gonideinae taxa was supported by all the inferred phylogenies in this study using, for the first time, the M- and F-type mitogenomes either singly or combined. Within Gonideinae, two additional splits in the gene order (UF1 to UF2, UF2 to UF3) occurred in the Mesozoic and have persisted for ~150 and ~100 Ma, respectively. Finally, the mitogenomic results suggest ancient connections between freshwater basins of East Asia and Europe near the Cretaceous–Paleogene boundary, probably via a continuous paleo-river system or along the Tethys coastal line, which are well supported by at least three independent but almost synchronous divergence events. ; The authors wish to thank the Editor and the three anonymous reviewers for helpful remarks and suggestions that improved the quality of the manuscript. This research was developed under ConBiomics: the missing approach for the Conservation of freshwater Bivalves Project N° NORTE-01-0145-FEDER-030286, cofinanced by COMPETE 2020, Portugal 2020 and the European Union through the ERDF, and by FCT through national funds (UID/Multi/04423/2019). FCT also supported MLL (SFRH/BD/115728/2016). The Russian Ministry of Education and Science (project no. 6.2343.2017/4.6), the Federal Agency for Scientific Organizations (project no. 0409-2015-0143), the Presidium of the Russian Academy of Sciences (scientific program no. 52), and the Russian Foundation for Basic Research, RFBR (project no. 17-45-290066) supported INB. Permits for fieldwork and sampling in Malaysia were issued by the Malaysian Ministry of Higher Education (FRGS/1/2015/WAB13/UNIM//1). ; info:eu-repo/semantics/publishedVersion

Aquatic ecosystem assessment is a rapidly developing field, and one of the newer approaches to assessing the condition of rivers and lakes is the Reference Condition Approach. This is a significant advancement in biomonitoring because it solves the problem of trying to locate nearby control or reference sites when studying an ecosystem that may be degraded, a problem that bedevils traditional approaches. Rather than using upstream reference sites in a river system or next-bay-over reference sites in a lake, an array of ecologically similar, least-exposed to stress sites scattered throughout a catchment or region is used. Once the reference condition has been established, any site suspected of being impacted can be assessed by comparison to the reference sites, and its status determined. The Reference Condition database, once formed, can be used repeatedly

The ranges and abundances of species that depend on freshwater habitats are declining worldwide. Efforts to counteract those trends are often hampered by a lack of information about species distribution and conservation status and are often strongly biased toward a few well-studied groups. We identified the 3,906 vascular plants, macroinvertebrates, and vertebrates native to California, USA, that depend on fresh water for at least one stage of their life history. We evaluated the conservation status for these taxa using existing government and non-governmental organization assessments (e.g., endangered species act, NatureServe), created a spatial database of locality observations or distribution information from ~400 data sources, and mapped patterns of richness, endemism, and vulnerability. Although nearly half of all taxa with conservation status (n = 1,939) are vulnerable to extinction, only 114 (6%) of those vulnerable taxa have a legal mandate for protection in the form of formal inclusion on a state or federal endangered species list. Endemic taxa are at greater risk than non-endemics, with 90% of the 927 endemic taxa vulnerable to extinction. Records with spatial data were available for a total of 2,276 species (61%). The patterns of species richness differ depending on the taxonomic group analyzed, but are similar across taxonomic level. No particular taxonomic group represents an umbrella for all species, but hotspots of high richness for listed species cover 40% of the hotspots for all other species and 58% of the hotspots for vulnerable freshwater species. By mapping freshwater species hotspots we show locations that represent the top priority for conservation action in the state. This study identifies opportunities to fill gaps in the evaluation of conservation status for freshwater taxa in California, to address the lack of occurrence information for nearly 40% of freshwater taxa and nearly 40% of watersheds in the state, and to implement adequate protections for freshwater taxa where they are currently lacking.

AbstractThis paper reviews the current state-of-the-art, limitations, critical issues, and new directions in freshwater plant ecotoxicology. We selected peer-reviewed studies using relevant databases and for each (1) publication year, (2) test plant species, (3) reference plant group (microalgae, macroalgae, bryophytes, pteridophytes, flowering plants), (4) toxicant tested (heavy metal, pharmaceutical product, hydrocarbon, pesticide, surfactant, plastic), (5) experiment site (laboratory, field), and (6) toxicant exposure duration. Although aquatic plant organisms play a key role in the functioning of freshwater ecosystems, mainly linked to their primary productivity, their use as biological models in ecotoxicological tests was limited if compared to animals. Also, toxicant effects on freshwater plants were scarcely investigated and limited to studies on microalgae (80%), or only to a certain number of recurrent species (Pseudokirchneriella subcapitata, Chlorella vulgaris, Lemna minor, Myriophyllum spicatum). The most widely tested toxicants on plants were heavy metals (74%), followed by pharmaceutical products and hydrocarbons (7%), while the most commonly utilized endpoints in tests were plant growth inhibition, variations in dry or fresh weight, morpho-structural alterations, chlorosis, and/or necrosis. The main critical issues emerged from plant-based ecotoxicological tests were the narrow range of species and endpoints considered, the lack of environmental relevance, the excessively short exposure times, and the culture media potentially reacting with toxicants. Proposals to overcome these issues are discussed.