Suchergebnisse

This new volume of Methods in Cell Biology looks at lipid droplets LDs, covering sections on analyses of LDs in model systems, cell/tissue-specific analyses of LDs and imaging and in vitro analyses of LD biogenesis and growth. Chapters are written by experts in the field. With cutting-edge material, this comprehensive collection is intended to guide researchers of LDs for years to come. Covers sections on analyses of lipid droplets (LDs) in model systems, cell/tissue-specific analyses of LDs and imaging, and in vitro analyses of LD biogenesis and growth Chapters are written by experts in the field Cutting-edge material

There is a general lack of understanding of the role of lipids in aquatic ecosystems. The fundamental purpose of this book is to synthesize the divergent literature on aquatic lipids into a coordinated, digestible form. A large part of the book addresses lipid composition and production in freshwater organisms, with chapters on phytoplankton, zooplankton and benthic invertebrates. A common theme throughout the book is the function of lipids in aquatic food webs, with a chapter devoted exclusively to lipids as indicators of health in fish populations. A complementary chapter highlights the role of lipids and essential fatty acids in mariculture. Methodologies to determine the lipid content of aquatic samples and suggestions as to the utility of fatty acids as trophic markers are provided. There is a chapter on the role of lipids in the bioaccumulation and bioconcentration of toxicants and another on the relationships between lipids and surface films and foams. The final chapter highlights the similarities and differences between lipids of marine and freshwater origin. Students and researchers in ecology, phycology, aquatic toxicology, physiological ecology and limnology will find this an invaluable guide and reference

1 Lipids: what they are and how the biochemist deals with them -- 1.1 General introduction -- 1.2 Nomenclature and stereochemistry -- 1.3 Analytical techniques -- 1.4 Bibliography -- 2 Fatty acids -- 2.1 Structure and properties -- 2.2 The biochemistry of fatty acids -- 2.3 Summary -- 2.4 Bibliography -- 3 Neutral Lipids: glycerides, sterol esters, vitamin A esters, waxes -- 3.1 Glycerides -- 3.2 Sterol esters -- 3.3 Vitamin A esters (retinyl esters) -- 3.4 Waxes -- 3.5 Analytical techniques -- 3.6 Summary -- 3.7 Bibliography -- 4 Phospholipids -- 4.1 Classification of phospholipids -- 4.2 Structure and occurrence -- 4.3 Biosynthesis of phospholipids -- 4.4 Degradation of phospholipids -- 4.5 Analysis of phospholipids -- 4.6 Summary -- 4.7 Bibliography -- 5 Glycolipids and sulpholipids -- 5.1 Glycosyl ceramides -- 5.2 Glycosyl glycerides -- 5.3 Other glycolipids and sulpholipids -- 5.4 The analysis of glycolipid mixtures -- 5.5 Summary -- 5.6 Bibliography -- 6 Lipids as components of macromolecules -- 6.1 Physical and chemical properties of lipids -- 6.2 Physical and chemical properties of proteins -- 6.3 Association of lipids and proteins -- 6.4 Naturally-occurring lipoproteins — 'soluble types' -- 6.5 Naturally-occurring lipoproteins — 'membrane types' -- 6.6 Lipopolysaccharides and bacterial cell walls -- 6.7 Summary -- 6.8 Bibliography -- 7 Lipids in nutrition, health and disease -- 7.1 Introduction: the diverse roles of dietary lipids -- 7.2 Source and composition of dietary lipids -- 7.3 Different roles of minor lipids in food -- 7.4 The metabolism of lipids in relation to disease -- 7.5 Summary -- 7.6 Bibliography -- General index -- Supplementary index of diseases -- Supplementary index of species -- Supplementary index of tissues.

Offers an account of the properties of the classes of lipids that are located in the membranes of various cells. This title presents a balanced perspective of what is known about the function of these lipids in healthy cells and tissues and what consequences follow malfunctions in their synthesis and metabolism.

1 Introduction -- 1A General remarks -- 1B Classification of organisms -- 1C Lipid nomenclature -- 2 Major Lipid Types in Plants and Micro-Organisms -- 2A Fatty acids -- 2B Acyl lipids -- 2C Terpenoids -- 2D Sterols -- 2E Other lipid types -- 2F Ether lipids -- 3 Distribution of Lipids -- 3A Lipid distributions in different organisms and their use in taxonomy -- 3B Subcellular distribution of lipids -- 3C Intramembrane lipid distribution -- 3D Subcellular fractionation and membrane isolation -- 3E Factors affecting the lipid composition of plants and microorganisms -- 4 Biosynthesis -- 4A Fatty acids -- 4B Acyl lipids -- 4C Terpenoids and steroids -- 4D Complex lipids -- 4E Ether lipids -- 5 Degradation -- 5A Degradation of acyl lipids -- 5B Oxidation of fatty acids -- 6 Lipid Functions -- 6A Membrane structure and function -- 6B Storage -- 6C Microbial lipids as virulence factors -- 6D Conclusion -- Further reading.

Peroxisomes, lipids, and neurodegeneration
Prof. Michael Schrader at the University of Exeter looks to understand the role of peroxisomes in human health and disease. The proper functioning of eukaryotic cells depends on membrane-bound subcellular compartments (organelles). They create distinct and unique reaction chambers with an optimised environment to promote various metabolic reactions required to sustain life. These compartments are highly dynamic and can alter their position, number, enzymatic content, and morphology according to cellular needs or environmental changes. In addition, organelles interact, communicate, and cooperate in metabolic pathways and tasks distributed across different organelles. Prof. Schrader and his team in the Dept. of Biosciences at the University of Exeter are investigating how these processes are regulated, and what molecular components and mechanisms are involved, with a particular focus on peroxisomes.

To clarify the relationship between neutral lipid content and cordycepin accumulation in Cordyceps militaris, mutants were generated from mixed spores of two C. militaris strains with varying cordycepin-producing capacities. Fifteen stable mutants producing from 0.001 to 2.363 mg/mL cordycepin were finally selected. The relative fluorescence intensities of the 15 mutants, two C. militaris strains and an Aspergillus nidulans strain at different concentrations of lyophilized mycelium powder were then investigated using the Nile red method. The mutant CM1-1-1 with the highest relative fluorescence intensity among the eighteen strains was selected for optimizing the Nile red method. Relative fluorescence intensity was linearly correlated with cordycepin concentration in liquid broth (R2 = 0.9514) and in lyophilized mycelium powder (R2 = 0.9378) for the 18 cordycepin-producing strains under identical culture conditions and with cordycepin concentration in liquid broth (R2 = 0.9727) and in lyophilized mycelium powder (R2 = 0.9613) for CM1-1-1 under eight different sets of conditions. In addition, the cordycepin content in lyophilized mycelium powder measured by the Nile red method was linearly correlated with that determined by an HPLC method (R2 = 0.9627). In conclusion, neutral lipids in lipid droplets are required during cordycepin accumulation ; these neutral lipids are potential biomarkers of cordycepin biosynthesis.