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Semaphorin 3A (Sema3A) is a cell-secreted protein that participates in the axonal guidance pathways. Sema3A acts as a canonical repulsive axon guidance molecule, inhibiting CNS regenerative axonal growth and propagation. Therefore, interfering with Sema3A signaling is proposed as a therapeutic target for achieving functional recovery after CNS injuries. It has been shown that Sema3A adheres to the proteoglycan component of the extracellular matrix (ECM) and selectively binds to heparin and chondroitin sulfate-E (CS-E) glycosaminoglycans (GAGs). We hypothesize that the biologically relevant interaction between Sema3A and GAGs takes place at Sema3A C-terminal polybasic region (SCT). The aims of this study were to characterize the interaction of the whole Sema3A C-terminal polybasic region (Sema3A 725–771) with GAGs and to investigate the disruption of this interaction by small molecules. Recombinant Sema3A basic domain was produced and we used a combination of biophysical techniques (NMR, SPR, and heparin affinity chromatography) to gain insight into the interaction of the Sema3A C-terminal domain with GAGs. The results demonstrate that SCT is an intrinsically disordered region, which confirms that SCT binds to GAGs and helps to identify the specific residues involved in the interaction. NMR studies, supported by molecular dynamics simulations, show that a new peptoid molecule (CSIC02) may disrupt the interaction between SCT and heparin. Our structural study paves the way toward the design of new molecules targeting these protein–GAG interactions with potential therapeutic applications. ; This work was funded by the European Union Seventh Framework Programme (FP7/2007–2013) under Project VISION, grant No. 304884, the Spanish Ministry of Science and Innovation/Spanish Research Agency (MCI/AEI/FEDER, RTI2018–096182-B-I00) and AGAUR (2017 SGR 208). ; Peer reviewed

Treballs Finals del Màster d'Advocacia, Facultat de Dret, Universitat de Barcelona, Curs: 2019-2020, Tutor: Dos Santos V. Factor, Javier

10 páginas.- 4 figuras.- referencias.-Data Availability Statement: All data underlying this article are available in the main publication and in its Supplementary Materials online. ; Unspecific peroxygenases (UPOs), the extracellular enzymes capable of oxygenating a potpourri of aliphatic and aromatic substrates with a peroxide as co-substrate, come out with a new reaction: carbon-chain shortening during the conversion of fatty acids with the well-known UPOs from Coprinopsis cinerea (rCciUPO) and Cyclocybe (Agrocybe) aegerita (AaeUPO). Although a pathway (Cα-oxidation) for shortening the hydrocarbon chain of saturated fatty acids has already been reported for the UPO from Marasmius rotula (MroUPO), it turned out that rCciUPO and AaeUPO shorten the chain length of both saturated and unsaturated fatty acids in a different way. Thus, the reaction sequence does not necessarily start at the Cα-carbon (adjacent to the carboxyl group), as in the case of MroUPO, but proceeds through the subterminal (ω-1 and ω-2) carbons of the chain via several oxygenations. This new type of shortening leads to the formation of a dicarboxylic fatty acid reduced in size by two carbon atoms in the first step, which can subsequently be further shortened, carbon by carbon, by the UPO Cα-oxidation mechanism. View Full-Text ; This research was funded by BioBased Industries Joint Undertaking under the European Union's Horizon 2020 Research and Innovation Programme, grant number 792063 (SusBind project; https://susbind.eu; to A.G., Á.T.M. and M.H.), the PID2020-118968RB-100 project by the Spanish MCIN/AEI/ 10.13039/501100011033 to A.G., and the CSIC projects PIE-202040E185 (to A.G.) and PIE-202120E019 (to Á.T.M.). ; Peer reviewed

At the end of the 1870s, the oppressive policies of the Argentine State were intensified towards the indigenous people linked to the Southern Border, visible in the punitive expeditions of 1878-1879. In the case of theranqueles, located in the centralPampa, these actions were part of previous strategies of attrition, carried out by the military commanders ofSan LuisandCórdobathrough the diplomatic channel, softening campaigns and co-optation of contingents who, both forced and voluntarily, migrated to the fortsVilla MercedesandSarmiento. From a line of investigation that seeks to reconstruct the itineraries of theranquelessubmitted in the aforementioned context, the article characterizes the imprisonment of the indigenous people taken by the 3rd Expeditionary Division (April-August 1879) under the leadership of Colonel Eduardo Racedo. Its purpose is to describe their living conditions while they were in thePitral Lauquencamp (nearPoitagué), understanding that it simultaneously acted as a place of concentration and distribution of prisoners. ; Al terminar la década de 1870 se intensificaron las políticas opresivas del Estado argentino para con los indígenas ligados a la Frontera Sur, visibles en las expediciones punitivas de 1878-1879. En el caso de los ranqueles, situados en la pampa central, estas acciones se enmarcan en estrategias previas de desgaste ejecutadas por los mandos militares de San Luis y Córdoba a través de la vía diplomática, las campañas de ablandamiento y cooptación de contingentes que migrabande manera forzaday por presentación a los fuertes Villa Mercedes y Sarmiento. Desde una línea de investigación que busca reconstruir los itinerarios de los ranqueles sometidos en ese contexto, el artículo caracteriza elaprisionamiento de los indígenas tomados por la 3aDivisión Expedicionaria (abril-agosto de 1879) bajo el liderazgo del coronel Eduardo Racedo. Su finalidad es describir sus condiciones de vida mientras estuvieron depositados en el campamento Pitral Lauquen (cercano a Poitagué), lugar de concentración y distribución de prisioneros.

For ensuring full access to the journal's contents, we provide this link in order to download the full issue. ; Para facilitar al máximo el acceso de los lectores a los contenidos de laRevista Universitaria de Historia Militarproporcionamos este enlace directo para la descarga del número completo.

The writings of the Indian Diaspora have received a great deal of attention and critical acclaim throughout the world. Rohinton Mistry is a well renowned author in the contemporary commonwealth literature and occupies a significant place among the writers of Indian Diaspora. Even though he is settled in Canada; it is his upbringing in Mumbai that reflects in all his writings. The distinct Mumbai culture, particularly the Parsee way of life, the people of the city and even the politics of India are major themes in his novels. Rohinton Mistry as a writer of Diaspora has carved a niche for himself. His works such as: "Tales from Firozsha Baag", "Such A Long Journey", "A Fine Balance" and "Family Matters"; mark a new kind of writing, resulting from a fragmented, splintered world. As a Diasporic Parsi writer, very sensitively he has recalled his community's journey through time and history with a sense of loss and nostalgia. His books portray diverse facets of Indian socioeconomic life; as well as Parsi Zoroastrian life, customs, and religion. Many of his writings are markedly "Indo-nostalgic". Rohinton Mistry through his Diasporic discourse has well depicted his ancestral background, his community's engaged situation in a metropolis like Bombay and his deep attachment with and nostalgia for a world gone by. In this paper an attempt has been made to delineate diasporic discourse in the works of Rohinton Mistry. Although he is obsessed with the colonial and postcolonial experiences in India, we can find some traces of Canada in his literature. His characters dream of being integrated into, and accepted by, Canadian society on the one hand and on the other hand, these same characters are torn by an insatiable desire to be true to their native culture; to honor and cherish their own, distinct cultural identity.Keywords: Diaspora, Cultural identity, Contemporary Canadian literature, Parsi.

With new proposals and development initiatives, the government aims to maintain the momentum of economic growth. There is emphasis on keeping poverty and job losses to a minimum with no new taxes, expansion of social protection schemes, and increase in salaries. Initiatives to protect both formal and informal Small and Medium Enterprises (SMEs) have been announced in view of the stresses faced by private enterprises amid COVID-19.

This study evaluates the consolidated financial condition and performance of local water districts (LWDs) in the Philippines. Water districts are government-owned or controlled corporations (GOCCs) tasked to construct, operate, maintain, and expand water and sanitation systems in the countryside. They are instrumental in the Philippines' objective to attain 100-percent water supply and sanitation access by 2036. National data show that the consolidated financial performance of LWDs has improved from 2009 to 2018, as reflected in their high and stable cash flows, high debt service coverage ratios, and lower debt ratios. With the aggressive spending program of the government on water infrastructure, lower debt ratios are needed to prepare LWDs to achieve the 2023 and the 2030 goals of universal access to water supply and sanitation. However, the government's spending plans are so ambitious that the current balance sheets of LWDs cannot sustain the planned investments financed through debt. The national government needs to bolster the balance sheets of LWDs by infusing fresh equity of PHP 22 billion to PHP 56 billion to achieve the Philippines' 2030 water supply and sanitation targets. The study likewise shows a significant disparity in water investments across the country's different regions, which impacts the uneven water service coverage throughout the Philippines.

This study evaluates the consolidated financial condition and performance of local water districts (LWDs) in the Philippines. Water districts are government-owned or controlled corporations (GOCCs) tasked to construct, operate, maintain, and expand water and sanitation systems in the countryside. They are instrumental in the Philippines' objective to attain 100-percent water supply and sanitation access by 2036. National data show that the consolidated financial performance of LWDs has improved from 2009 to 2018, as reflected in their high and stable cash flows, high debt service coverage ratios, and lower debt ratios. With the aggressive spending program of the government on water infrastructure, lower debt ratios are needed to prepare LWDs to achieve the 2023 and the 2030 goals of universal access to water supply and sanitation. However, the government's spending plans are so ambitious that the current balance sheets of LWDs cannot sustain the planned investments financed through debt. The national government needs to bolster the balance sheets of LWDs by infusing fresh equity of PHP 22 billion to PHP 56 billion to achieve the Philippines' 2030 water supply and sanitation targets. The study likewise shows a significant disparity in water investments across the country's different regions, which impacts the uneven water service coverage throughout the Philippines.

This week's guest is Ryan Busse, former senior executive in the firearms industry. Ryan's new book, Gunfight, is an intimate and revealing account of his experience in that industry, his growing disillusionment with it and his ultimate exit. In this conversation, we talk about rural household gun culture, the symbolism assault rifles take on in political division, and the rights and responsibilities of gun ownership in our country. ; https://scholarworks.umt.edu/anewangle_podcasts/1224/thumbnail.jpg

This report looks at the upcoming redistricting cycle through the lens of four factors that will influence outcomes in each state: who controls map drawing; changes in the legal rules governing redistricting over the last decade; pressures from population and demographic shifts over the same period; and the potential impact of the Covid-19 pandemic on the 2020 Census. In each state, the confluence of these factors will determine the risk of manipulated maps or whether, conversely, the redistrict[1]ing process will produce maps that reflect what voters want, respond to shifts in public opinion, and protect the rights of communities of color.

Attached are updated "Information Guides" for each type of tax appeal that briefly explain the procedures outlined in SC Revenue Procedure #20-1. The guides are intended as a quick reference tool for taxpayer's and tax practitioners.

Domestic Violence in Immigrant Communities: Case Studies" is a freely accessible eCampus Ontario Pressbook containing case studies of immigrant women experiencing domestic violence to be used as educational materials. The contents were created by analysing closed legal case files of 15 immigrant women living in Ontario who experienced domestic violence. The comprehensive case studies that emerge from this research present domestic violence experienced by immigrant women in all its complexity, highlighting their unique vulnerability at the intersections of race, gender and immigration status. The book also highlights the different legal processes that these women encounter in seeking justice and the challenges they face in relation to re-establishing their own lives and the lives of their children. In addition to the cases, the book contains questions for reflection; a description of legal processes involved in DV cases, and a glossary of the terms used throughout the case studies. This interactive Pressbook is an ideal resource for social work and legal practitioners, including students in social service work, social work and law programs, in order to increase their understanding about the complexity of domestic violence cases in immigrant families and develop strategies for culturally informed interventions. ; Chaze, F., J, B., Medhekar, A., George, P., & Chahal, K. (2020). Domestic violence in immigrant communities: Case studies. [Ontario], eCampusOntario.

Abstract: Advocacy is an integral part of child and youth care workers' roles and a significant component of child and youth care politicized praxis and radical youth work. Drawing from the qualitative data of a mixed-methods study conducted in 2019 at a Canadian metropolitan university, this study seeks to unpack how the pedagogy of the lightning talk can foster advocacy skills to effectively and spontaneously speak out with and on behalf of children, youth, and families in everyday practice when an unforeseen systemic challenge or barrier arises. A purposive sample of 70 undergraduate students was recruited in two child and youth care courses, both of which required students to present a lightning talk. Participants completed an online questionnaire with closed-ended and open-ended questions in order to share their perspectives of the pedagogy of the lightning talk. The findings show that the lightning talk fosters twenty-first century and metacognitive skills and, most importantly, advocacy skills. Keywords: pedagogy, lightning talk, oral presentations, advocacy, child and youth care, youth work ; Jean-Pierre, J., Hassan, S., Sturge, A., Gharabaghi, K., Lewis, M., Bailey, J. & Panitch, M. (2020). Poised to advocate: the pedagogy of the lightning talk in child and youth care education. International Journal of Child, Youth and Family Studies, 11(3), 108–125. https://doi.org/10.18357/ijcyfs113202019703

The Covid-19 pandemic has had a broad impact on the global tourism world since the end of 2019. Data shows that international tourist arrivals declined sharply in the first half of 2020 (-65%) compared to 2019. The strategy has been implemented by various special countries, the Indonesian Ministry of Tourism proclaimed the principle of Adapting to New Habits (AKB) in the Indonesian tourism industry as an integral part of the permit to reopen tourism businesses and destinations. The IMR principles in Indonesian tourism are applied from a strategic and technical perspective as well as from supply chain changes to the needs and demands of the management of tourists. This research was produced to be able to see the readiness of tourist destinations in the region, especially Tourism Villages in dealing with the Covid-19 situation and conditions with several strategies that have been set by the central government starting from CHSE and digitalization for Tourism Villages. This study used a quantitative research method with a description that describes each goal starting from the 3A condition, the Health Protocol, and its digitization. This mapping is very important to determine the direction of the potential development of Cihanjawar Tourism Village as a tourist destination