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Semaphorin 3A (Sema3A) is a cell-secreted protein that participates in the axonal guidance pathways. Sema3A acts as a canonical repulsive axon guidance molecule, inhibiting CNS regenerative axonal growth and propagation. Therefore, interfering with Sema3A signaling is proposed as a therapeutic target for achieving functional recovery after CNS injuries. It has been shown that Sema3A adheres to the proteoglycan component of the extracellular matrix (ECM) and selectively binds to heparin and chondroitin sulfate-E (CS-E) glycosaminoglycans (GAGs). We hypothesize that the biologically relevant interaction between Sema3A and GAGs takes place at Sema3A C-terminal polybasic region (SCT). The aims of this study were to characterize the interaction of the whole Sema3A C-terminal polybasic region (Sema3A 725–771) with GAGs and to investigate the disruption of this interaction by small molecules. Recombinant Sema3A basic domain was produced and we used a combination of biophysical techniques (NMR, SPR, and heparin affinity chromatography) to gain insight into the interaction of the Sema3A C-terminal domain with GAGs. The results demonstrate that SCT is an intrinsically disordered region, which confirms that SCT binds to GAGs and helps to identify the specific residues involved in the interaction. NMR studies, supported by molecular dynamics simulations, show that a new peptoid molecule (CSIC02) may disrupt the interaction between SCT and heparin. Our structural study paves the way toward the design of new molecules targeting these protein–GAG interactions with potential therapeutic applications. ; This work was funded by the European Union Seventh Framework Programme (FP7/2007–2013) under Project VISION, grant No. 304884, the Spanish Ministry of Science and Innovation/Spanish Research Agency (MCI/AEI/FEDER, RTI2018–096182-B-I00) and AGAUR (2017 SGR 208). ; Peer reviewed

10 páginas.- 4 figuras.- referencias.-Data Availability Statement: All data underlying this article are available in the main publication and in its Supplementary Materials online. ; Unspecific peroxygenases (UPOs), the extracellular enzymes capable of oxygenating a potpourri of aliphatic and aromatic substrates with a peroxide as co-substrate, come out with a new reaction: carbon-chain shortening during the conversion of fatty acids with the well-known UPOs from Coprinopsis cinerea (rCciUPO) and Cyclocybe (Agrocybe) aegerita (AaeUPO). Although a pathway (Cα-oxidation) for shortening the hydrocarbon chain of saturated fatty acids has already been reported for the UPO from Marasmius rotula (MroUPO), it turned out that rCciUPO and AaeUPO shorten the chain length of both saturated and unsaturated fatty acids in a different way. Thus, the reaction sequence does not necessarily start at the Cα-carbon (adjacent to the carboxyl group), as in the case of MroUPO, but proceeds through the subterminal (ω-1 and ω-2) carbons of the chain via several oxygenations. This new type of shortening leads to the formation of a dicarboxylic fatty acid reduced in size by two carbon atoms in the first step, which can subsequently be further shortened, carbon by carbon, by the UPO Cα-oxidation mechanism. View Full-Text ; This research was funded by BioBased Industries Joint Undertaking under the European Union's Horizon 2020 Research and Innovation Programme, grant number 792063 (SusBind project; https://susbind.eu; to A.G., Á.T.M. and M.H.), the PID2020-118968RB-100 project by the Spanish MCIN/AEI/ 10.13039/501100011033 to A.G., and the CSIC projects PIE-202040E185 (to A.G.) and PIE-202120E019 (to Á.T.M.). ; Peer reviewed

At the end of the 1870s, the oppressive policies of the Argentine State were intensified towards the indigenous people linked to the Southern Border, visible in the punitive expeditions of 1878-1879. In the case of theranqueles, located in the centralPampa, these actions were part of previous strategies of attrition, carried out by the military commanders ofSan LuisandCórdobathrough the diplomatic channel, softening campaigns and co-optation of contingents who, both forced and voluntarily, migrated to the fortsVilla MercedesandSarmiento. From a line of investigation that seeks to reconstruct the itineraries of theranquelessubmitted in the aforementioned context, the article characterizes the imprisonment of the indigenous people taken by the 3rd Expeditionary Division (April-August 1879) under the leadership of Colonel Eduardo Racedo. Its purpose is to describe their living conditions while they were in thePitral Lauquencamp (nearPoitagué), understanding that it simultaneously acted as a place of concentration and distribution of prisoners. ; Al terminar la década de 1870 se intensificaron las políticas opresivas del Estado argentino para con los indígenas ligados a la Frontera Sur, visibles en las expediciones punitivas de 1878-1879. En el caso de los ranqueles, situados en la pampa central, estas acciones se enmarcan en estrategias previas de desgaste ejecutadas por los mandos militares de San Luis y Córdoba a través de la vía diplomática, las campañas de ablandamiento y cooptación de contingentes que migrabande manera forzaday por presentación a los fuertes Villa Mercedes y Sarmiento. Desde una línea de investigación que busca reconstruir los itinerarios de los ranqueles sometidos en ese contexto, el artículo caracteriza elaprisionamiento de los indígenas tomados por la 3aDivisión Expedicionaria (abril-agosto de 1879) bajo el liderazgo del coronel Eduardo Racedo. Su finalidad es describir sus condiciones de vida mientras estuvieron depositados en el campamento Pitral Lauquen (cercano a Poitagué), lugar de concentración y distribución de prisioneros.

For ensuring full access to the journal's contents, we provide this link in order to download the full issue. ; Para facilitar al máximo el acceso de los lectores a los contenidos de laRevista Universitaria de Historia Militarproporcionamos este enlace directo para la descarga del número completo.

The writings of the Indian Diaspora have received a great deal of attention and critical acclaim throughout the world. Rohinton Mistry is a well renowned author in the contemporary commonwealth literature and occupies a significant place among the writers of Indian Diaspora. Even though he is settled in Canada; it is his upbringing in Mumbai that reflects in all his writings. The distinct Mumbai culture, particularly the Parsee way of life, the people of the city and even the politics of India are major themes in his novels. Rohinton Mistry as a writer of Diaspora has carved a niche for himself. His works such as: "Tales from Firozsha Baag", "Such A Long Journey", "A Fine Balance" and "Family Matters";mark a new kind of writing, resulting from a fragmented, splintered world. As a Diasporic Parsi writer, very sensitively he has recalled his community's journey through time and history with a sense of loss and nostalgia. His books portray diverse facets of Indian socioeconomic life; as well as Parsi Zoroastrian life, customs, and religion. Many of his writings are markedly "Indo-nostalgic". Rohinton Mistry through his Diasporic discourse has well depicted his ancestral background, his community's engaged situation in a metropolis like Bombay and his deep attachment with and nostalgia for a world gone by. In this paper an attempt has been made to delineate diasporic discourse in the works of Rohinton Mistry. Although he is obsessed with the colonial and postcolonial experiences in India, we can find some traces of Canada in his literature. His characters dream of being integrated into, and accepted by, Canadian society on the one hand and on the other hand, these same characters are torn by an insatiable desire to be true to their native culture; to honor and cherish their own, distinct cultural identity.

The writings of the Indian Diaspora have received a great deal of attention and critical acclaim throughout the world. Rohinton Mistry is a well renowned author in the contemporary commonwealth literature and occupies a significant place among the writers of Indian Diaspora. Even though he is settled in Canada; it is his upbringing in Mumbai that reflects in all his writings. The distinct Mumbai culture, particularly the Parsee way of life, the people of the city and even the politics of India are major themes in his novels. Rohinton Mistry as a writer of Diaspora has carved a niche for himself. His works such as: "Tales from Firozsha Baag", "Such A Long Journey", "A Fine Balance" and "Family Matters"; mark a new kind of writing, resulting from a fragmented, splintered world. As a Diasporic Parsi writer, very sensitively he has recalled his community's journey through time and history with a sense of loss and nostalgia. His books portray diverse facets of Indian socioeconomic life; as well as Parsi Zoroastrian life, customs, and religion. Many of his writings are markedly "Indo-nostalgic". Rohinton Mistry through his Diasporic discourse has well depicted his ancestral background, his community's engaged situation in a metropolis like Bombay and his deep attachment with and nostalgia for a world gone by. In this paper an attempt has been made to delineate diasporic discourse in the works of Rohinton Mistry. Although he is obsessed with the colonial and postcolonial experiences in India, we can find some traces of Canada in his literature. His characters dream of being integrated into, and accepted by, Canadian society on the one hand and on the other hand, these same characters are torn by an insatiable desire to be true to their native culture; to honor and cherish their own, distinct cultural identity.Keywords: Diaspora, Cultural identity, Contemporary Canadian literature, Parsi.

Throughout this internship, I, Abhi Pasupula, have worked with my internship mentor, Barry Federici, in order to help him start up a new service. This service is targeted specifically towards veterans and their paths in their lives after they retire from the military. The service is split up into two categories, those being Jobs and Veteran Benefits. Jobs entailed creating and implement a job board into our website for retired veterans to search for. Veteran benefits showcase a list of benefits that veterans are eligible for, divided up by Federal Benefits, State Benefits, Local Benefits, and a page for all available benefits. For the Job Board page on the website, we got into contact with a job board service known as Hiring Opps and spent many days working through the features and seeing which features would serve us the best for the website. In addition, we set up a Sandbox so that we could physically see the service in action. The benefits required more menial work, such as compiling the list of total benefits and categorizing them into states with links that lead to the state Veteran Benefits commission for more information. Once organized, the benefits were organized into 4 sections, each section having its own page on the website. Both of these websites were connected back to the original website, which served as a homepage for all the services. The homepage also had services to meet with my mentor, Mr. Federici. Working on both of these websites and services really opened my eyes to the professional world of Software Development, where there was so much more apart from just programming. Similar to this internship, the real world will require me to be able to voice my thoughts as well as put them down on paper and be able to explain them well to others, something that I believe this internship set me up for very well. ; https://digitalcommons.imsa.edu/intern_reports_2021/1004/thumbnail.jpg

With new proposals and development initiatives, the government aims to maintain the momentum of economic growth. There is emphasis on keeping poverty and job losses to a minimum with no new taxes, expansion of social protection schemes, and increase in salaries. Initiatives to protect both formal and informal Small and Medium Enterprises (SMEs) have been announced in view of the stresses faced by private enterprises amid COVID-19.

This study evaluates the consolidated financial condition and performance of local water districts (LWDs) in the Philippines. Water districts are government-owned or controlled corporations (GOCCs) tasked to construct, operate, maintain, and expand water and sanitation systems in the countryside. They are instrumental in the Philippines' objective to attain 100-percent water supply and sanitation access by 2036. National data show that the consolidated financial performance of LWDs has improved from 2009 to 2018, as reflected in their high and stable cash flows, high debt service coverage ratios, and lower debt ratios. With the aggressive spending program of the government on water infrastructure, lower debt ratios are needed to prepare LWDs to achieve the 2023 and the 2030 goals of universal access to water supply and sanitation. However, the government's spending plans are so ambitious that the current balance sheets of LWDs cannot sustain the planned investments financed through debt. The national government needs to bolster the balance sheets of LWDs by infusing fresh equity of PHP 22 billion to PHP 56 billion to achieve the Philippines' 2030 water supply and sanitation targets. The study likewise shows a significant disparity in water investments across the country's different regions, which impacts the uneven water service coverage throughout the Philippines.

This study evaluates the consolidated financial condition and performance of local water districts (LWDs) in the Philippines. Water districts are government-owned or controlled corporations (GOCCs) tasked to construct, operate, maintain, and expand water and sanitation systems in the countryside. They are instrumental in the Philippines' objective to attain 100-percent water supply and sanitation access by 2036. National data show that the consolidated financial performance of LWDs has improved from 2009 to 2018, as reflected in their high and stable cash flows, high debt service coverage ratios, and lower debt ratios. With the aggressive spending program of the government on water infrastructure, lower debt ratios are needed to prepare LWDs to achieve the 2023 and the 2030 goals of universal access to water supply and sanitation. However, the government's spending plans are so ambitious that the current balance sheets of LWDs cannot sustain the planned investments financed through debt. The national government needs to bolster the balance sheets of LWDs by infusing fresh equity of PHP 22 billion to PHP 56 billion to achieve the Philippines' 2030 water supply and sanitation targets. The study likewise shows a significant disparity in water investments across the country's different regions, which impacts the uneven water service coverage throughout the Philippines.

In der deutschen Rechtswissenschaft scheint bereits die Idee von Open Access (OA) einen schweren Stand zu haben: Eine Reihe von Wissenschaftler:innen üben offen Kritik an der Idee oder bezweifeln sogar die Verfassungsmäßigkeit von Open-Access-Vorgaben oder -Richtlinien. Nichtsdestotrotz sind in den letzten Jahren innovative digitale Formate entstanden, die hochwertige rechtswissenschaftliche Inhalte OA veröffentlichen. Nennenswerte Beispiele sind der Verfassungsblog und der Völkerrechtsblog. Diese Plattformen nennen sich "Blogs", stellen als qualitätsgesicherte Multi-Author-Blogs (QMABs) aber tatsächlich eine neue, innovative, frei verfügbare und genuin digitale Form des wissenschaftlichen Publizierens dar, die zwischen wissenschaftlichen Zeitschriften und traditionellen Medien/Presse angesiedelt ist. In einem QMAB können Expert:innen (in der Regel Wissenschaftler:innen) tagesaktuell Ereignisse analysieren, einordnen und kommentieren. QMABs in der Rechtswissenschaft und den Geistes- und Sozialwissenschaften haben als innovatives Publikationsformat in den vergangenen Jahren eine wachsende Zahl an Autor:innen und Leser:innen hinzugewonnen. Auch die breite Öffentlichkeit nimmt zunehmend Notiz von diesen Plattformen. Gesellschaftspolitische Entwicklungen und Ereignisse sind häufig mit rechtlichen und rechtspolitischen Fragestellungen verknüpft, auf die auch Nicht-Wissenschaftler:innen Antworten suchen. Gleichzeitig haben Wissenschaftler:innen ein Interesse daran, ihre Expertise in den politischen Raum zu tragen. QMAB dienen somit nicht nur dem wissenschaftlichen Binnendiskurs, sondern ermöglichen es auch der breiten Öffentlichkeit, an Fachdiskursen teilzunehmen, etwa über die Kommentarfunktion – und all dies über staatliche Grenzen hinweg. Damit fördern QMABs nicht nur den wissenschaftlichen Austausch und öffnen Diskurse für ein breites Publikum, sondern internationalisieren diese auch. Hierin offenbaren sie ihr partizipatives und kommunikatives Potenzial, das letztlich dem wissenschaftlichen Erkenntnisgewinn zugute ...

This week's guest is Ryan Busse, former senior executive in the firearms industry. Ryan's new book, Gunfight, is an intimate and revealing account of his experience in that industry, his growing disillusionment with it and his ultimate exit. In this conversation, we talk about rural household gun culture, the symbolism assault rifles take on in political division, and the rights and responsibilities of gun ownership in our country. ; https://scholarworks.umt.edu/anewangle_podcasts/1224/thumbnail.jpg

This report looks at the upcoming redistricting cycle through the lens of four factors that will influence outcomes in each state: who controls map drawing; changes in the legal rules governing redistricting over the last decade; pressures from population and demographic shifts over the same period; and the potential impact of the Covid-19 pandemic on the 2020 Census. In each state, the confluence of these factors will determine the risk of manipulated maps or whether, conversely, the redistrict[1]ing process will produce maps that reflect what voters want, respond to shifts in public opinion, and protect the rights of communities of color.

Un nuevo número de nuestra revista vuelve a encontrarnos en un clima de protesta y rebelión en nuestra región, por muchos años de destrucción de lazos sociales, culturales y políticos acompañados de brutales represiones y violaciones a los DDHH en el hermano país de Colombia, y en plena pandemia. Como en nuestro número 20 -dedicado a la rebelión chilena de octubre 2019 (Alvarado Lincopi y Robles Recabarren, 2020)- hoy nos solidarizamos con les compañeres colombianes que enfrentan todavía, desde el 28 de abril, una brutal violencia por parte de las fuerzas policiales y militares del Estado, del ESMAD y de los comandos civiles armados, protegidos por la fuerza pública. Casi podemos decir que presenciamos las escenas que se difundieron ampliamente por las redes sociales, por videos y por exitosos llamados a la solidaridad para la difusión de los acontecimientos. Compartimos, así, desde lejos la emoción por el coraje de las comunidades indígenas, las mujeres, les jóvenes, les estudiantes, les docentes y demás colectivos movilizados. Alentamos la esperanza de que el pueblo colombiano sea escuchado, que se detenga la masacre y se retome el espíritu de los Acuerdos de Paz, de una Paz que se sustente en el reconocimiento de derechos y no en la negación de ellos. #S.O.S. COLOMBIA recorre el mundo. Este número presenta, justamente, un dossier que nos habla de la organización sistemática de la represión y persecución de opositores como especialidad y estructura de la inteligencia represiva, de los archivos y las burocracias del Mal, de las lógicas del funcionamiento de las fuerzas represivas y de la sistematización de la información sobre el "enemigo", construido a partir de diversas doctrinas. El Dossier "Los organismos de inteligencia en Argentina. Miradas desde los archivos a una burocracia secreta", coordinado por las colegas Mariana Nazar y María Eva Muzzoppapa, despliega el interés y el análisis de ese "oscuro y arbitrario accionar de las agencias de inteligencia" nucleando a un conglomerado profesional de archivistas -de archivistas políticas- preocupadas por la activación de políticas de archivo y de inquietudes y responsabilidades historiográficas. ; Facultad de Humanidades y Ciencias de la Educación

This thesis studies fictional representations of Fyodor Dostoevsky in contemporary biofiction. The aim of the study is to present an intermedial theoretical framework for biofiction, a genre defined as fictional biographical and often metafictional narratives in which a biographical subject serves as the focal point for the story or plays a role integral to the narrative. Drawing on contributions from prior studies within different areas—biopics, the biographical novel, intermediality, transmedial narratology—the thesis identifies the most salient tenets of an increasingly important and ubiquitous genre—its fictional, intermedial, and metafictional properties—to suggest a medium-spanning definition. From this intermedial definition of the genre, it is suggested that biofiction studies should move beyond medium-specific analysis. By situating the genre firmly within the realm of fiction, the thesis underlines the fact that it is exactly the fictional element that allows the genre to open up important ways to engage with a certain biographical subject. Overall, the biofiction definition presented in the thesis is inspired by Jacques Derrida's différance. Arguing that contemporary biofiction arose from the larger aporetic shift in theory and fiction in the 1960s, which was directed toward various presuppositions undergirding epistemological, metaphysical, and ontological projects, it is contended that biofiction fictionalizes subjects to engage with and reassess the assumptions that suffuse our understanding of the subject. From their metafictional perspective, biofictions also employ subjects for various purposes to interrogate contemporary issues. Biofictions are thus turned toward both a historical moment and its own contemporary context. Buttressed by the intermedial perspective, it is argued that biofictions often employ sustained intermedial strategies—for instance, intermedial references and formal imitation—to engage not only with artistic subjects such as Dostoevsky and their work but also with the premises of creating art. The thesis centers on five Dostoevsky biofictions within film and literature: Aleksandr Zarkhi's biopic Twenty-Six Days from the Life of Dostoevsky (1980), Leonid Tsypkin's novel Summer in Baden-Baden (1982), J. M. Coetzee's novel The Master of Petersburg (1994), Lara Vapnyar's novel Memoirs of a Muse (2006), and Vladimir Khotinenko's television series Dostoevsky (2010). As contemporary biofictions, these fictional representations of Dostoevsky were all produced or written in the wake of the aforementioned aporetic shift and therefore comprise examples of the reflexive and metacritical form of biofiction that is discussed in the thesis. The inclusion of Dostoevsky biofictions is, in part, connected with the various critical perceptions of the writer; it is maintained that biofictions such as those analyzed in the thesis proffer new readings of issues that have been overlooked or have not received due attention, such as how Dostoevsky engaged with and augmented the rivalry polemics of his day; the ways in which his conceptualization of Russian identity rested as much on inclusion as exclusion; how Dostoevsky has been employed to propagate certain models of the muse, the genius, and canonicity; and how, in today's Russia, the writer is employed to embody and express the hyperreal politics of Vladimir Putin's administration.