Suchergebnisse

Semaphorin 3A (Sema3A) is a cell-secreted protein that participates in the axonal guidance pathways. Sema3A acts as a canonical repulsive axon guidance molecule, inhibiting CNS regenerative axonal growth and propagation. Therefore, interfering with Sema3A signaling is proposed as a therapeutic target for achieving functional recovery after CNS injuries. It has been shown that Sema3A adheres to the proteoglycan component of the extracellular matrix (ECM) and selectively binds to heparin and chondroitin sulfate-E (CS-E) glycosaminoglycans (GAGs). We hypothesize that the biologically relevant interaction between Sema3A and GAGs takes place at Sema3A C-terminal polybasic region (SCT). The aims of this study were to characterize the interaction of the whole Sema3A C-terminal polybasic region (Sema3A 725–771) with GAGs and to investigate the disruption of this interaction by small molecules. Recombinant Sema3A basic domain was produced and we used a combination of biophysical techniques (NMR, SPR, and heparin affinity chromatography) to gain insight into the interaction of the Sema3A C-terminal domain with GAGs. The results demonstrate that SCT is an intrinsically disordered region, which confirms that SCT binds to GAGs and helps to identify the specific residues involved in the interaction. NMR studies, supported by molecular dynamics simulations, show that a new peptoid molecule (CSIC02) may disrupt the interaction between SCT and heparin. Our structural study paves the way toward the design of new molecules targeting these protein–GAG interactions with potential therapeutic applications. ; This work was funded by the European Union Seventh Framework Programme (FP7/2007–2013) under Project VISION, grant No. 304884, the Spanish Ministry of Science and Innovation/Spanish Research Agency (MCI/AEI/FEDER, RTI2018–096182-B-I00) and AGAUR (2017 SGR 208). ; Peer reviewed

10 páginas.- 4 figuras.- referencias.-Data Availability Statement: All data underlying this article are available in the main publication and in its Supplementary Materials online. ; Unspecific peroxygenases (UPOs), the extracellular enzymes capable of oxygenating a potpourri of aliphatic and aromatic substrates with a peroxide as co-substrate, come out with a new reaction: carbon-chain shortening during the conversion of fatty acids with the well-known UPOs from Coprinopsis cinerea (rCciUPO) and Cyclocybe (Agrocybe) aegerita (AaeUPO). Although a pathway (Cα-oxidation) for shortening the hydrocarbon chain of saturated fatty acids has already been reported for the UPO from Marasmius rotula (MroUPO), it turned out that rCciUPO and AaeUPO shorten the chain length of both saturated and unsaturated fatty acids in a different way. Thus, the reaction sequence does not necessarily start at the Cα-carbon (adjacent to the carboxyl group), as in the case of MroUPO, but proceeds through the subterminal (ω-1 and ω-2) carbons of the chain via several oxygenations. This new type of shortening leads to the formation of a dicarboxylic fatty acid reduced in size by two carbon atoms in the first step, which can subsequently be further shortened, carbon by carbon, by the UPO Cα-oxidation mechanism. View Full-Text ; This research was funded by BioBased Industries Joint Undertaking under the European Union's Horizon 2020 Research and Innovation Programme, grant number 792063 (SusBind project; https://susbind.eu; to A.G., Á.T.M. and M.H.), the PID2020-118968RB-100 project by the Spanish MCIN/AEI/ 10.13039/501100011033 to A.G., and the CSIC projects PIE-202040E185 (to A.G.) and PIE-202120E019 (to Á.T.M.). ; Peer reviewed

The writings of the Indian Diaspora have received a great deal of attention and critical acclaim throughout the world. Rohinton Mistry is a well renowned author in the contemporary commonwealth literature and occupies a significant place among the writers of Indian Diaspora. Even though he is settled in Canada; it is his upbringing in Mumbai that reflects in all his writings. The distinct Mumbai culture, particularly the Parsee way of life, the people of the city and even the politics of India are major themes in his novels. Rohinton Mistry as a writer of Diaspora has carved a niche for himself. His works such as: "Tales from Firozsha Baag", "Such A Long Journey", "A Fine Balance" and "Family Matters"; mark a new kind of writing, resulting from a fragmented, splintered world. As a Diasporic Parsi writer, very sensitively he has recalled his community's journey through time and history with a sense of loss and nostalgia. His books portray diverse facets of Indian socioeconomic life; as well as Parsi Zoroastrian life, customs, and religion. Many of his writings are markedly "Indo-nostalgic". Rohinton Mistry through his Diasporic discourse has well depicted his ancestral background, his community's engaged situation in a metropolis like Bombay and his deep attachment with and nostalgia for a world gone by. In this paper an attempt has been made to delineate diasporic discourse in the works of Rohinton Mistry. Although he is obsessed with the colonial and postcolonial experiences in India, we can find some traces of Canada in his literature. His characters dream of being integrated into, and accepted by, Canadian society on the one hand and on the other hand, these same characters are torn by an insatiable desire to be true to their native culture; to honor and cherish their own, distinct cultural identity.Keywords: Diaspora, Cultural identity, Contemporary Canadian literature, Parsi.

With new proposals and development initiatives, the government aims to maintain the momentum of economic growth. There is emphasis on keeping poverty and job losses to a minimum with no new taxes, expansion of social protection schemes, and increase in salaries. Initiatives to protect both formal and informal Small and Medium Enterprises (SMEs) have been announced in view of the stresses faced by private enterprises amid COVID-19.

This study evaluates the consolidated financial condition and performance of local water districts (LWDs) in the Philippines. Water districts are government-owned or controlled corporations (GOCCs) tasked to construct, operate, maintain, and expand water and sanitation systems in the countryside. They are instrumental in the Philippines' objective to attain 100-percent water supply and sanitation access by 2036. National data show that the consolidated financial performance of LWDs has improved from 2009 to 2018, as reflected in their high and stable cash flows, high debt service coverage ratios, and lower debt ratios. With the aggressive spending program of the government on water infrastructure, lower debt ratios are needed to prepare LWDs to achieve the 2023 and the 2030 goals of universal access to water supply and sanitation. However, the government's spending plans are so ambitious that the current balance sheets of LWDs cannot sustain the planned investments financed through debt. The national government needs to bolster the balance sheets of LWDs by infusing fresh equity of PHP 22 billion to PHP 56 billion to achieve the Philippines' 2030 water supply and sanitation targets. The study likewise shows a significant disparity in water investments across the country's different regions, which impacts the uneven water service coverage throughout the Philippines.

This study evaluates the consolidated financial condition and performance of local water districts (LWDs) in the Philippines. Water districts are government-owned or controlled corporations (GOCCs) tasked to construct, operate, maintain, and expand water and sanitation systems in the countryside. They are instrumental in the Philippines' objective to attain 100-percent water supply and sanitation access by 2036. National data show that the consolidated financial performance of LWDs has improved from 2009 to 2018, as reflected in their high and stable cash flows, high debt service coverage ratios, and lower debt ratios. With the aggressive spending program of the government on water infrastructure, lower debt ratios are needed to prepare LWDs to achieve the 2023 and the 2030 goals of universal access to water supply and sanitation. However, the government's spending plans are so ambitious that the current balance sheets of LWDs cannot sustain the planned investments financed through debt. The national government needs to bolster the balance sheets of LWDs by infusing fresh equity of PHP 22 billion to PHP 56 billion to achieve the Philippines' 2030 water supply and sanitation targets. The study likewise shows a significant disparity in water investments across the country's different regions, which impacts the uneven water service coverage throughout the Philippines.

This week's guest is Ryan Busse, former senior executive in the firearms industry. Ryan's new book, Gunfight, is an intimate and revealing account of his experience in that industry, his growing disillusionment with it and his ultimate exit. In this conversation, we talk about rural household gun culture, the symbolism assault rifles take on in political division, and the rights and responsibilities of gun ownership in our country. ; https://scholarworks.umt.edu/anewangle_podcasts/1224/thumbnail.jpg

This report looks at the upcoming redistricting cycle through the lens of four factors that will influence outcomes in each state: who controls map drawing; changes in the legal rules governing redistricting over the last decade; pressures from population and demographic shifts over the same period; and the potential impact of the Covid-19 pandemic on the 2020 Census. In each state, the confluence of these factors will determine the risk of manipulated maps or whether, conversely, the redistrict[1]ing process will produce maps that reflect what voters want, respond to shifts in public opinion, and protect the rights of communities of color.

Attached are updated "Information Guides" for each type of tax appeal that briefly explain the procedures outlined in SC Revenue Procedure #20-1. The guides are intended as a quick reference tool for taxpayer's and tax practitioners.

Domestic Violence in Immigrant Communities: Case Studies" is a freely accessible eCampus Ontario Pressbook containing case studies of immigrant women experiencing domestic violence to be used as educational materials. The contents were created by analysing closed legal case files of 15 immigrant women living in Ontario who experienced domestic violence. The comprehensive case studies that emerge from this research present domestic violence experienced by immigrant women in all its complexity, highlighting their unique vulnerability at the intersections of race, gender and immigration status. The book also highlights the different legal processes that these women encounter in seeking justice and the challenges they face in relation to re-establishing their own lives and the lives of their children. In addition to the cases, the book contains questions for reflection; a description of legal processes involved in DV cases, and a glossary of the terms used throughout the case studies. This interactive Pressbook is an ideal resource for social work and legal practitioners, including students in social service work, social work and law programs, in order to increase their understanding about the complexity of domestic violence cases in immigrant families and develop strategies for culturally informed interventions. ; Chaze, F., J, B., Medhekar, A., George, P., & Chahal, K. (2020). Domestic violence in immigrant communities: Case studies. [Ontario], eCampusOntario.

Abstract: Advocacy is an integral part of child and youth care workers' roles and a significant component of child and youth care politicized praxis and radical youth work. Drawing from the qualitative data of a mixed-methods study conducted in 2019 at a Canadian metropolitan university, this study seeks to unpack how the pedagogy of the lightning talk can foster advocacy skills to effectively and spontaneously speak out with and on behalf of children, youth, and families in everyday practice when an unforeseen systemic challenge or barrier arises. A purposive sample of 70 undergraduate students was recruited in two child and youth care courses, both of which required students to present a lightning talk. Participants completed an online questionnaire with closed-ended and open-ended questions in order to share their perspectives of the pedagogy of the lightning talk. The findings show that the lightning talk fosters twenty-first century and metacognitive skills and, most importantly, advocacy skills. Keywords: pedagogy, lightning talk, oral presentations, advocacy, child and youth care, youth work ; Jean-Pierre, J., Hassan, S., Sturge, A., Gharabaghi, K., Lewis, M., Bailey, J. & Panitch, M. (2020). Poised to advocate: the pedagogy of the lightning talk in child and youth care education. International Journal of Child, Youth and Family Studies, 11(3), 108–125. https://doi.org/10.18357/ijcyfs113202019703

The Covid-19 pandemic has had a broad impact on the global tourism world since the end of 2019. Data shows that international tourist arrivals declined sharply in the first half of 2020 (-65%) compared to 2019. The strategy has been implemented by various special countries, the Indonesian Ministry of Tourism proclaimed the principle of Adapting to New Habits (AKB) in the Indonesian tourism industry as an integral part of the permit to reopen tourism businesses and destinations. The IMR principles in Indonesian tourism are applied from a strategic and technical perspective as well as from supply chain changes to the needs and demands of the management of tourists. This research was produced to be able to see the readiness of tourist destinations in the region, especially Tourism Villages in dealing with the Covid-19 situation and conditions with several strategies that have been set by the central government starting from CHSE and digitalization for Tourism Villages. This study used a quantitative research method with a description that describes each goal starting from the 3A condition, the Health Protocol, and its digitization. This mapping is very important to determine the direction of the potential development of Cihanjawar Tourism Village as a tourist destination

Scottish Recommended Lists for Cereals 2021/22 The Scottish cereals lists include varieties of spring and winter barley, wheat and oats that are of most agronomic and commercial value to Scottish growers and the cereals sector. Recommendations are made by SRUC supported by the Scottish Variety Consultative Committee and are based on data collected as part of the AHDB Recommended List and Scottish Government National List system. Highlights for 2021/22 The spring barley list has been consolidated with the main malting choices being Laureate and LG Diablo, both dual-purpose distilling and brewing varieties, and KWS Sassy, a distilling variety. Fairing remains on the list as the only fully approved grain distilling variety. Looking ahead, SY Tungsten and Firefoxx continue to make progress as they are evaluated commercially for malting use. The only new spring barley variety is Skyway, which has potential for brewing. The winter barley list includes three new two-row feed varieties, KWS Tardis, Bolton and Bordeaux, plus two new six-row hybrids, SY Kingston and SY Thunderbolt. The leading two-row feed choices are LG Mountain, KWS Orwell, KWS Tower, Valerie and KWS Hawking. The Scottish winter wheat list has increased to eighteen varieties, twelve of which are suitable for the grain distilling market. The leading distilling varieties are LG Skyscraper, Elation and KWS Jackal, supported by the biscuit-making variety Elicit. There are five new distilling varieties, Swallow, a soft feed variety, and soft-milling choices, LG Prince, LG Illuminate, LG Quasar and LG Astronomer. The other new entry to the list is a spring feed wheat variety, WPB Escape. There are no new spring or winter oat varieties. Sixteen varieties have been removed from the lists because of limited market interest, or low agronomic value. These are spring barley varieties Concerto, RGT Asteroid, Propino and Scholar; winter barleys KWS Cassia, KWS Creswell, LG Flynn, Jordan and Libra; winter wheat varieties Zulu, KWS Lili and Grafton and spring oats Firth, Yukon, Delfin and Elison.

This thesis studies fictional representations of Fyodor Dostoevsky in contemporary biofiction. The aim of the study is to present an intermedial theoretical framework for biofiction, a genre defined as fictional biographical and often metafictional narratives in which a biographical subject serves as the focal point for the story or plays a role integral to the narrative. Drawing on contributions from prior studies within different areas—biopics, the biographical novel, intermediality, transmedial narratology—the thesis identifies the most salient tenets of an increasingly important and ubiquitous genre—its fictional, intermedial, and metafictional properties—to suggest a medium-spanning definition. From this intermedial definition of the genre, it is suggested that biofiction studies should move beyond medium-specific analysis. By situating the genre firmly within the realm of fiction, the thesis underlines the fact that it is exactly the fictional element that allows the genre to open up important ways to engage with a certain biographical subject. Overall, the biofiction definition presented in the thesis is inspired by Jacques Derrida's différance. Arguing that contemporary biofiction arose from the larger aporetic shift in theory and fiction in the 1960s, which was directed toward various presuppositions undergirding epistemological, metaphysical, and ontological projects, it is contended that biofiction fictionalizes subjects to engage with and reassess the assumptions that suffuse our understanding of the subject. From their metafictional perspective, biofictions also employ subjects for various purposes to interrogate contemporary issues. Biofictions are thus turned toward both a historical moment and its own contemporary context. Buttressed by the intermedial perspective, it is argued that biofictions often employ sustained intermedial strategies—for instance, intermedial references and formal imitation—to engage not only with artistic subjects such as Dostoevsky and their work but also with the premises of creating art. The thesis centers on five Dostoevsky biofictions within film and literature: Aleksandr Zarkhi's biopic Twenty-Six Days from the Life of Dostoevsky (1980), Leonid Tsypkin's novel Summer in Baden-Baden (1982), J. M. Coetzee's novel The Master of Petersburg (1994), Lara Vapnyar's novel Memoirs of a Muse (2006), and Vladimir Khotinenko's television series Dostoevsky (2010). As contemporary biofictions, these fictional representations of Dostoevsky were all produced or written in the wake of the aforementioned aporetic shift and therefore comprise examples of the reflexive and metacritical form of biofiction that is discussed in the thesis. The inclusion of Dostoevsky biofictions is, in part, connected with the various critical perceptions of the writer; it is maintained that biofictions such as those analyzed in the thesis proffer new readings of issues that have been overlooked or have not received due attention, such as how Dostoevsky engaged with and augmented the rivalry polemics of his day; the ways in which his conceptualization of Russian identity rested as much on inclusion as exclusion; how Dostoevsky has been employed to propagate certain models of the muse, the genius, and canonicity; and how, in today's Russia, the writer is employed to embody and express the hyperreal politics of Vladimir Putin's administration.

This information guide explains the South Carolina Tax Appeals Procedure for State Tax Refund Claims (Other than Property Tax, Bingo, and Alcoholic Beverage Matters).