Suchergebnisse

Projeto EXPOsE – Establishing protocols to assess occupational exposure to microbiota in clinical settings (02/SAICT/2016 – Project nº 23222). ; Studies have reported that there is a significant relationship between hospital infections and bioaerosols. Fungi are present in clinical indoor environments and play important roles in human diseases in patients and staff. Aspergillus is a common fungus in invasive infections, however, other fungi are enrolled as pathogens in immunosuppressed patients. The azole-resistant Aspergillus sp. has up to 30% prevalence in some European hospitals, which report higher than 90% mortality rates. Control measures are crucial in clinical environments for reducing concentrations of airborne fungal contamination and to avoid invasive infections acquired from indoor air. We cannot fully eliminate fungi from indoor clinical environments and enforcement is needed to ensure, at least, proper Indoor Air Quality (IAQ) assessments. Portuguese legislation established limit values for air bioburden in indoor environments in general, without any specific requirement for health care facilities. This study focuses on the mycobiota assessment in 10 Primary Health Care Centers (PHCC) applying the IAQ Portuguese legislation as guidance. After the quantitative cut-off analyses (I/O) the fungal species identification was performed to verify if the fungal conformity was achieved even in those that comply with the I/O ratio. ; info:eu-repo/semantics/acceptedVersion

O Transtorno de Déficit de Atenção e Hiperatividade (TDAH) caracteriza-se como uma condição neurobiológica de causas genéticas que ocasiona sintomas como desatenção, hiperatividade e impulsividade. Compreendendo que esses sintomas afetam principalmente as crianças em idade escolar, este estudo tem como objetivo analisar a produção de trabalhos nos anais do Encontro Nacional de Ensino de Biologia (ENEBIO) relacionada a produções acadêmicas sobre a inclusão de alunos com TDAH no ensino de Ciências e Biologia. Trata-se de uma pesquisa de caráter qualitativo, do tipo levantamento bibliográfico, com um recorte temporal de 10 anos, sendo correspondente ao período de 2011 a 2021. A busca dos trabalhos se deu a partir dos seguintes descritores: TDA/H, transtorno, déficit de atenção, hiperatividade, educação especial, educação inclusiva. Os resultados apontam que há uma baixa quantidade de artigos publicados no ENEBIO sobre inclusão de alunos com o transtorno, evidenciando a necessidade de novos estudos. O baixo número de resultados dificulta as discussões sobre a temática, bem como a elaboração de estratégias didático-pedagógicas que possibilitem a inclusão de estudantes com TDAH no ensino de Ciências e Biologia.

Project EXPOsE – Establishing protocols to assess occupational exposure to microbiota in clinical settings (02/SAICT/2016 – Project nº 23222). ; Introduction - Clinical settings (hospitals, primary health care centers – PHCC), with high occupation rates, present microbiologic agents in their environment. Exposure to mycobiota in indoor environments is related to several adverse human health effects, such as respiratory symptoms. Azole resistance in fungal species and consequent failure of antifungal therapy is a major concern for public health. Portuguese legislation recommends active air sampling for bioburden assessment indoors. Passive sampling can be used in complement to determine bioburden levels from longer periods. Aim of the study - To assess the fungal burden and prevalence of azole resistance in clinical settings in Portugal using passive sampling methods. ; info:eu-repo/semantics/publishedVersion

Project "Waste Workers' Exposure to Bioburden in the Truck Cab during Waste Management - W2E Bioburden" (IPL/2016/W2E_ESTeSL). ; Project "Occupational exposure of ambulance drivers to bioburden" (IPL/2020/BIO-AmbuDrivers_ESTeSL). ; FCT_UIDB/05608/2020. FCT_UIDP/05608/2020. ; Best poster award ; Introduction: The microbial exposure, as well as their metabolic products, has been frequently reported as a high risk of respiratory effects and general health hazards. Regarding the Portuguese legislation for IAQ, the microbial assessment covers only air sampling and culture-based methods (malt extract agar as only culture media to be applied). Fungal legal criteria consist of a fungal load ratio between indoor and outdoor, which should be lower than 1 (I/O<1). Purpose: Analyze the importance of including the characterization of the azole resistance profile in indoor air quality assessments. ; info:eu-repo/semantics/publishedVersion

This study aims to assess the airborne bioburden of rural and urban Portuguese Primary Health Care Centers (PHCC) using active and passive sampling methods and identify the potential differences in airborne microbiota between both environments. The highest total aerobic mesophilic bacterial load in indoor air were found in the Vaccination Room (448 CFU.m−3) in the Rural PHCC and in the Waiting Room (420 CFU.m−3) for Urban PHCC. The total coliforms contamination level in indoor air was detected only in the Cleaning Supplies Room (4 CFU.m−3) in the Urban PHCC. The most frequent bacteria genera identified was Micrococcus (21% Rural PHCC; 31% Urban PHCC). The surface samples showed a highest total aerobic mesophilic bacterial contamination in the Treatment Room (86 × 103 CFU.m−2) from the Rural PHCC and in the Front Office (200 × 103 CFU.m−2) from the Urban PHCC. The electrostatic dust cloth (EDC) samples showed a highest bacterial load in the Urban PHCC. Total aerobic mesophilic bacterial load in settled dust and in the Heating, Ventilating and Air Conditioning (HVAC) filter samples in the Urban PHCC (8 CFU.g−1 and 6 × 103 CFU.m−2) presented higher values compared with the Rural PHCC (1 CFU.g−1 and 2.5 × 103 CFU.m−2). Urban PHCC presented higher bacterial airborne contamination compared with the Rural PHCC for the ma-jority of sampling sites, and when compared with the indoor air quality (IAQ) Portuguese legislation it was the Rural PHCC in two sampling places who did not comply with the established criteria. ; publishersversion ; published

Project EXPOsE, nº 23222 (02/SAICT/2016) ; This study aims to assess the airborne bioburden of rural and urban Portuguese Primary Health Care Centers (PHCC) using active and passive sampling methods and identify the potential differences in airborne microbiota between both environments. The highest total aerobic mesophilic bacterial load in indoor air was found in the Vaccination Room (448 CFU.m−3) in the Rural PHCC and in the Waiting Room (420 CFU.m−3) for Urban PHCC. The total coliform contamination level in indoor air was detected only in the Cleaning Supplies Room (4 CFU.m−3) in the Urban PHCC. The most frequent bacteria genera identified was Micrococcus (21% Rural PHCC; 31% Urban PHCC). The surface samples showed the highest total aerobic mesophilic bacterial contamination in the Treatment Room (86 × 103 CFU.m−2) from the Rural PHCC and in the Front Office (200 × 103 CFU.m−2) from the Urban PHCC. The electrostatic dust cloth (EDC) samples showed the highest bacterial load in the Urban PHCC. Total aerobic mesophilic bacterial load in settled dust and in the Heating, Ventilating, and Air Conditioning (HVAC) filter samples in the Urban PHCC (8 CFU.g−1 and 6 × 103 CFU.m−2) presented higher values compared with the Rural PHCC (1 CFU.g−1 and 2.5 × 103 CFU.m−2). Urban PHCC presented higher bacterial airborne contamination compared with the Rural PHCC for the majority of sampling sites, and when compared with the indoor air quality (IAQ) Portuguese legislation it was the Rural PHCC in two sampling places that did not comply with the established criteria. ; info:eu-repo/semantics/publishedVersion

The exposure to particles and bioaerosols has been associated with the increase in health effects in children. The objective of this study was to assess the indoor exposure to bioburden in the indoor microenvironments more frequented by children. Air particulate matter (PM) and settled dust were sampled in 33 dwellings and four schools with a medium volume sampler and with a passive method using electrostatic dust collectors (EDC), respectively. Settled dust collected by EDC was analyzed by culture-based methods (including azole resistance profile) and using qPCR. Results showed that the PM2.5 and PM10 concentrations in classrooms (31.15 μg/m3 and 57.83 μg/m3, respectively) were higher than in homes (15.26 μg/m3 and 18.95 μg/m3, respectively) and highly exceeded the limit values established by the Portuguese legislation for indoor air quality. The fungal species most commonly found in bedrooms was Penicillium sp. (91.79%), whereas, in living rooms, it was Rhizopus sp. (37.95%). Aspergillus sections with toxigenic potential were found in bedrooms and living rooms and were able to grow on VOR. Although not correlated with PM, EDC provided information regarding the bioburden. Future studies, applying EDC coupled with PM assessment, should be implemented to allow for a long-term integrated sample of organic dust. ; publishersversion ; published

LIFE Index-Air project (LIFE15 ENV/PT/000674). FCT_UID/Multi/04349/2019. ; The exposure to particles and bioaerosols has been associated with the increase in health effects in children. The objective of this study was to assess the indoor exposure to bioburden in the indoor microenvironments more frequented by children. Air particulate matter (PM) and settled dust were sampled in 33 dwellings and four schools with a medium volume sampler and with a passive method using electrostatic dust collectors (EDC), respectively. Settled dust collected by EDC was analyzed by culture-based methods (including azole resistance profile) and using qPCR. Results showed that the PM2.5 and PM10 concentrations in classrooms (31.15 μg/m3 and 57.83 μg/m3, respectively) were higher than in homes (15.26 μg/m3 and 18.95 μg/m3, respectively) and highly exceeded the limit values established by the Portuguese legislation for indoor air quality. The fungal species most commonly found in bedrooms was Penicillium sp. (91.79%), whereas, in living rooms, it was Rhizopus sp. (37.95%). Aspergillus sections with toxigenic potential were found in bedrooms and living rooms and were able to grow on VOR. Although not correlated with PM, EDC provided information regarding the bioburden. Future studies, applying EDC coupled with PM assessment, should be implemented to allow for a long-term integrated sample of organic dust. ; info:eu-repo/semantics/publishedVersion

As disciplinas do eixo de Saúde Pública, do curso de Odontologia da Universidade Federal de Santa Catarina (UFSC), têm como princípio formar indivíduos comprometidos com o Sistema Único de Saúde (SUS). Com a finalidade de capacitá-los a serem futuros transformadores da realidade atual, por meio do conhecimento sobre epidemiologia, planejamento e execução de políticas públicas. O objetivo deste trabalho é relatar a experiência dos alunos no planejamento e execução das atividades realizadas em conjunto com as Unidades Básicas de Saúde (UBSs), do município de Florianópolis. Os estudantes realizaram ações de promoção de saúde bucal e exames clínicos em escolares, além de participarem do planejamento e processo de trabalho das UBSs. A presença dos estudantes na rede pública de saúde contribui para capacitar futuros profissionais da saúde capazes de desenvolver práticas mais efetivas para as necessidades da comunidade e a serem futuros transformadores sociais da realidade do SUS.

The interplay between biochemical characteristics and the generation of volatile compounds in 11 type II sourdough fermented by single strains of lactic acid bacteria (LAB) was studied. Samples were collected at 0, 6, 9, 12, 15, 18 and 24h for analyses of microbial growth, pH, titratable acidity and CO2 production. During the first 12h, the LABs entered the stationary phase, and the formation of organic and carboxyl acids, alcohols, and esters were observed. Although acidity is an important characteristic of sourdough, in this work increasing the acetic acid content decreased yeast growth and the CO2 retention capacity of the doughs. The main carbohydrate consumed by autochthonous yeast was influenced by the LAB added (homo-or heterofermentative), as observed by correlation analysis. Maltose and glucose showed a strong and negative correlation with the yeast cell density in the dough fermented by homo and heterofermentative LAB, respectively. Moreover, LAB had an important effect on the aromatic profile, being the alcohols, aldehydes, alkanes, organics acids and esters mainly groups characterized. Altogether, 100 different volatile compounds were identified; however, each dough had a different volatile profile. This study shows, for the first time, the influence of a single strain of LAB on the characteristics of type II sourdough.