Suchergebnisse

Background: Kaposi�s sarcoma (KS) remains themostcommonmalignancyamongHIV-infected patients. Humanherpesvirus type- 8 (HHV-8) is regarded as the infectious etiological agent of Kaposi�s sarcoma (KSHV). Diagnostic procedures associated with KSHV are not routinely performed in HIV-infected subjects. Objectives: The main objective of this study is to obtain information on KSHV epidemiology in Iranian HIV-infected individuals. Patients and Methods: In the present cross-sectional study, 109 patients with established HIV infection, who visited a governmental and referral center for HIV screening in Tehran (Tehran west health center (TWHC)) between May 2014 and July 2015 were enrolled according to the convenience sample strategy. After peripheral blood collection, isolation of plasma and peripheral blood mononuclear cell (PBMC) compartments, DNA extraction was performed. KSHV DNA was analyzed by nested polymerase chain reaction (nested PCR) using primers from ORF-26 (virus minor capsid). Results: Among all 109 HIV-infected patients, 67 (61.5) were male, with an age range of 2 - 64 years (mean±standard deviation 35.8 ±13.3). KSHV DNA was found in PBMC and plasma samples of six (5.5) and four (3.6) patients, respectively. Conclusions: This study revealed a considerable prevalence of KSHV DNA, during latent and lytic phases, among HIV-infected patients. Risk factors for KSHV infection acquisition and concurrent. 0+infection with HIV were also evaluated. Diagnosis of KSHV in the group could be helpful for prognosis of Kaposi�s sarcoma and clinical management. © 2016, Iranian Red Crescent Medical Journal.

Background: Military populations are more prone to respiratory infections worldwide. There is a dearth of research about the role of viral pathogens in the etiology of respiratory infections in military trainees in Iran. Hence, we aimed to investigate the molecular epidemiology and clinical symptoms of respiratory viruses among this population. Methods: This cross-sectional study was performed on 400 military trainees with symptoms of respiratory infection, referred to the military medical clinic center in the basic military training camp of the General Staff of the Armed Forces of the Islamic Republic of Iran. Nucleic acid extraction from the throat or nasopharyngeal swab samples was performed by an automated extraction system. The extracts were then analyzed by the CLART® PneumoVir array system for the detection of respiratory viruses. Results: All military trainees were male, aged between 18 and 57 years (mean: 21.69 years). Sore throat (75.5), rhinorrhea (63.2), cough (59.2), fever (59.2), and nasal congestion (50.5) were amongst the most common symptoms. Overall, viral pathogens were detected in a total count of 124 (31). The most commonly detected viruses were rhinovirus (7.2), respiratory syncytial virus A (7.2) and influenza B virus (6). Conclusion: This study was an important first step for understanding the etiological role of viral pathogens in respiratory infection among military trainees population in Iran. Our results indicated that rhinovirus, respiratory syncytial virus A and influenza B virus are important viral pathogens causing respiratory infection in military trainees, respectively. However, further multi-center studies with larger sample size are strongly recommended to confirm our findings. © Iran University of Medical Sciences.