Suchergebnisse

Lei Cai,1 Yan Li,2 Xue-wen Yang,1 Xiao Lian,1 Man Guo,1 Shu-ao Xiao,1 Wen-bin Wang,1 Hong-wei Zhang1 1Division of Digestive Surgery, Xijing Hospital of Digestive Diseases, Fourth Military Medical University, 2Department of Anesthesiology, Northwest Women's and Children's Hospital, Xi'an, China Background: The mucinous component is a special histologic factor in gastric adenocarcinoma. The aim of this study was to assess the prognostic significance of mucinous component in gastric adenocarcinoma according to proportion.Patients and methods: Candidate patients with gastric adenocarcinoma were given radical D2 gastrectomies from September 2008 to May 2015 in our division. Clinicopathologic data and prognosis were monitored and analyzed among gastric adenocarcinoma patients with various proportions of mucinous component.Results: A total of 690 gastric adenocarcinomas with various proportions of mucinous component from 6,025 gastric adenocarcinoma patients were included. Higher numbers of patients with mucinous component came from: young patients, females, those with drinking history, at lower locations, Borrmann type III and IV, T4 stage, and positive for dissected lymph nodes. Tumors and pathological molecular markers showed more positivity in CEA, CA19-9, S100, and CD34. As the various proportions increased, more mucinous component seemed to be accompanied by more Borrmann type III and IV, T4 stage, and more positive expression of CEA and CA19-9. However, no significant difference in 5-year overall survival rate was observed among various proportions or existence of mucinous component. Also, proportion or existence of mucinous component was not an independent prognostic factor in multivariate analysis.Conclusion: Mucinous component was not a prognostic factor for gastric adenocarcinoma after radical D2 gastrectomy, no matter what proportion the component comprised. However, gastric adenocarcinoma with mucinous component showed specific clinicopathological characteristics, such as more advanced tumor stage, different age and sex, and more positive rate of molecular markers, which might provide a new strategy for optimal individual diagnosis and therapies. Keywords: mucinous component, mucinous gastric carcinoma, gastric adenocarcinoma with mucinous component, pure gastric adenocarcinoma, radical D2 gastrectomy, prognosis

Guanghui Xu,1,* Fan Feng,1,* Shushang Liu,1,* Fei Wang,1,2 Gaozan Zheng,1 Qiao Wang,1,3 Lei Cai,1 Man Guo,1 Xiao Lian,1 Hongwei Zhang1 1Division of Digestive Surgery, Xijing Hospital of Digestive Diseases, The Fourth Military Medical University, Xi'an, Shaanxi, 2Department of General Surgery, No 534 Hospital of PLA, Luoyang, 3Department of Surgery, No 91 Center Hospital of PLA, Jiaozuo, Henan, China *These authors contributed equally to this work Background: Little is known about the clinicopathological features and prognosis in elderly gastric cancer (GC) patients aged 65–79 years. The aim of this study was to evaluate clinicopathological features and prognosis in elderly GC patients. Patients and methods: From May 2008 to December 2014, a total of 5,282 GC patients were enrolled in our present study. Patients were divided into elderly and middle-aged groups. The clinicopathological features and clinical outcomes were analyzed. Results: The proportion of dysphagia was significantly higher in elderly patients than that in middle-aged patients (P=0.002), whereas the proportion of abdominal pain and heartburn was significantly lower in elderly patients than that in middle-aged patients (P0.05). Age, tumor size, histological type, tumor depth, lymph node metastasis, carcinoembryonic antigen, alpha fetoprotein, CA19-9, and CA125 were independent risk factors for the prognosis of GC patients in univariate and multivariate analyses. Overall survival in elderly patients was significantly reduced compared with middle-aged patients (P=0.001), especially in patients with tumor size >5 cm (P=0.002), poorly differentiated tumor (P<0.000), stage III tumor (P=0.002), or normal levels of carcinoembryonic antigen (P=0.009), alpha fetoprotein (P=0.002), CA19-9 (P=0.002), and CA125 (P=0.004). Conclusion: The clinicopathological features of elderly patients were different to those of middle-aged patients. The prognosis for elderly GC patients was significantly worse than for middle-aged patients. Keywords: gastric cancer, elderly, clinicopathological features, prognosis

Artificial sweeteners can bind to the sweet taste receptors T1R2/T1R3 on the cell membrane of taste receptor cells, including intestinal enteroendocrine cells, and induce an increase in intracellular Ca2+ concentration ([Ca2+]i). Acute adaptation or a reduction in receptor signalling was observed after prolonged exposure to a taste stimulus in an animal model. However, at the cell level, it remains unclear whether the expression level of T1R2/T1R3 will be changed by pre-exposure to sweetener molecules, and whether the corresponding change in [Ca2+]i induced by the altered expression of sweet taste receptors varies. In the present work, western blotting and qPCR analysis showed that the expression level of T1R2/T1R3 in NCI-H716 cells was upregulated by sucralose stimulation. In the sucralose-treated cells, the accumulation of intracellular Ca2+ in the sweetener pre-treated cells was significantly enhanced as compared to that in cells without sweetener pre-treatment, although the sweet response differed between acesulfame K and sucralose with the same sweetness. Collectively, the increased expression of T1Rs in sweetener pre-incubated cells enhanced the sweet taste signal response, which implies that long-term administration of artificial sweeteners in beverages or foods could alter the sensitivity of taste receptor cells to sweetener molecules.

Multidentate molecular additives are widely used to passivate perovskite, yet the role of chelate effect is still unclear. Here, the authors investigate a wide range of additives with different coordination number and functional moieties to establish correlation between coordination affinity and perovskite crystallisation dynamics. Molecular additives are widely utilized to minimize non-radiative recombination in metal halide perovskite emitters due to their passivation effects from chemical bonds with ionic defects. However, a general and puzzling observation that can hardly be rationalized by passivation alone is that most of the molecular additives enabling high-efficiency perovskite light-emitting diodes (PeLEDs) are chelating (multidentate) molecules, while their respective monodentate counterparts receive limited attention. Here, we reveal the largely ignored yet critical role of the chelate effect on governing crystallization dynamics of perovskite emitters and mitigating trap-mediated non-radiative losses. Specifically, we discover that the chelate effect enhances lead-additive coordination affinity, enabling the formation of thermodynamically stable intermediate phases and inhibiting halide coordination-driven perovskite nucleation. The retarded perovskite nucleation and crystal growth are key to high crystal quality and thus efficient electroluminescence. Our work elucidates the full effects of molecular additives on PeLEDs by uncovering the chelate effect as an important feature within perovskite crystallization. As such, we open new prospects for the rationalized screening of highly effective molecular additives. ; Funding Agencies|ERC Starting GrantEuropean Research Council (ERC) [717026]; Swedish Energy Agency EnergimyndighetenSwedish Energy Agency [48758-1, 44651-1]; Swedish Research Council VRSwedish Research Council; NanoLund; Swedish Government Strategic Research Area in Materials Science on Functional Materials at Linkoping University [2009-00971]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [91833303, 61974098, 62005126]; National Key Research and Development Program [2016YFA0201900]; Jiangsu High Educational Natural Science Foundation [18KJA430012]; 111 ProgramMinistry of Education, China - 111 Project; Collaborative Innovation Center of Suzhou Nano Science and Technology; Collaborative Innovation Center of Suzhou Nano Science Technology; Research Foundation - Flanders (FWO)FWO [12Y7218N, 12Y7221N, G098319N]; KU Leuven Research FundKU Leuven [C14/19/079, 201806920071, 201906830040, 201608530162, 201806460021]; China Scholarship CouncilChina Scholarship Council