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Mangroves, dominating tropical intertidal zones and estuaries, are among the most salt tolerant plants, and propagate through reproductive units called propagules. Similarly to plant seeds, propagules may harbor beneficial bacteria. Our hypothesis was that mangroves, being able to grow into seawater, should harbor bacteria able to interact with the host and to exert positive effects under salt stress, which could be exploited to improve crop production. Therefore, we isolated bacterial endophytes from mangrove propagules with the aim to test whether these bacteria have a beneficial potential on their natural host and on different crops such as barley and rice, cultivated under salt stress. The 172 bacterial isolates obtained were screened for plant growth promotion (PGP) activities in vitro, and the 12 most promising isolates were tested on barley under non-axenic conditions and salt stress. Gordonia terrae KMP456-M40 was the best performing isolate, increasing ear weight by 65%. Based on the in vivo PGP activity and the root colonization ability, investigated by fluorescence in situ hybridization and confocal microscopy, three strains were additionally tested on mangrove propagule germination and on rice growth. The most effective strain was again G. terrae KMP456-M40, which enhanced the root length of mangrove seedlings and the biomass of salt-stressed rice under axenic conditions up to 65% and 62%, respectively. We demonstrated that propagules, the reproductive units of mangroves, host beneficial bacteria that enhance the potential of mangrove seedlings establishment and confer salt tolerance to cereal crops. ; RS and MC acknowledge Karl-Heinze Kogel (Giessen) for the use the confocal microscope at the Institute of Phytopathology, JLU-Giessen. The research leading to these results received funding from the European Union's Seventh Framework Programme under grant agreement no 311975 (MACUMBA) and from the European Union's Horizon 2020 Research and Innovation program under Grant Agreement no 688320 (MADFORWATER). This publication is based upon work supported by the King Abdullah University of Science and Technology (KAUST) and the Office of Sponsored Research (OSR) under Award No. OSR-2018-CARF-1973 to the Red Sea Research Center. RS received a "Master Thesis Scholarship" from the University of Milan. FM acknowledges personal support from the project "Unveiling plant-bacterium interaction for agriculture and bioremediation (NURTURE)" (Piano di Sostegno della Ricerca 2015–2017: Linea 2 - Dotazione annuale per attività istituzionali).

Activated synovial macrophages play a key role in Rheumatoid Arthritis (RA). Recent studies have shown that folate receptor beta (FRβ) is specifically expressed by activated macrophages. Therefore a folate-based nanodevice would provide the possibility of delivering therapeutic agents to activated macrophages without affecting normal cells and tissues. This study shows for the first time the sonochemical preparation of HSA nanocapsules avoiding toxic cross linking chemicals and emulsifiers used in other methods. Production of HSA nanocapsules was optimized leading to a diameter of 443.5 ± 9.0 nm and a narrow size distribution indicated by a polydispersity index (PDI) of 0.066 ± 0.080. Nanocapsules were surface modified with folic acid (FA) and the FA content was determined to be 0.38 and 6.42 molecules FA per molecule HSA, depending on the surplus of FA employed. Dynamic light scattering was used to determine size, PDI and zetapotential of the produced nanocapsules before and after surface modification. FA distribution on the surface of HSA nanocapsules was localized three-dimensionally after fluorescence labeling using confocal laser scanning microscopy (CLSM). Furthermore, specific binding and internalization of HSA nanocapsules by FRβ-positive and FRβ-negative macrophages, obtained from human peripheral blood mononuclear cells, was demonstrated by flow cytometry. FRβ-expressing macrophages showed an increased binding for FA-modified capsules compared with those without FA. ; This work has received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement NMP4-LA-2009-228827 NANOFOL. We thank the Instituto Portugues do Sangue (IPS, Porto, Portugal) for providing buffy coats from informed healthy volunteers and Exbio from Czech Republic for providing ...

A biological approach for degumming bamboo substrates has been assessed. The ability of various commercially available enzymes, including cellulase, xylanase, pectinase and mannanase, to hydrolyze bamboo powders was investigated. In addition, a commercial cellulase preparation was applied onto bamboo fibre bundles obtained by natural retting. It was found that almost all enzymes applied can use bamboo material as a substrate. Mild autoclave pre-treatment can enhance reducing sugar yield from different enzyme treatments. A most pronounced effect was observed with cellulase treatment in which the hydrolysis degree increased 1.7 fold as measured by reducing sugars for autoclave pre-treated bamboo powders versus non-treated powders after only a short period of incubation. The combined treatment of hemicellulase preparations showed no effect on the hydrolysis of bamboo substrates. The effect of autoclave pre-treatment on cellulase-treated samples was confirmed by the increase of sugar yield, protein absorption as well as by the enhancement of surface modification and enzyme penetration observed by CLSM (confocal laser scanning microscopy). This work establishes a base for future studies to develop enzymatic hydrolysis of bamboo materials, making them suitable for textile processing. ; This work was made possible by support from the earmarked fund for Modern Agro-industry Technology Research System (nycytx-19-E23), the European Union Biorenew Project [Sixth Framework Programme (FP6-2004-NMP-NI-4)] and the Fundamental Research Funds for the Central Universities ...

As a signatory to the World Summit on Sustainable Development (WSSD), the European Union (EU) has made a commitment to maintain or restore fish stocks to levels that can produce the maximum sustainable yield (MSY), and where possible not later than 2015. So how has the EU's Common Fisheries Policy (CFP) fared in trying to achieve this objective? The development of the status of 41 commercially exploited fish stocks from the North East Atlantic, North Sea and Baltic Sea (FAO Area 27) was analysed together with the economic performance of the fleets exploiting those stocks. The analyses indicate that the exploitation status for many of the stocks has greatly improved during the last 10 years while the economic performance of the fleets over the same period has been highly variable. The main economic indicators (gross value added (GVA) and operating cash flow (OCF)) have gradually improved at a time when the general economic situation, which has a great influence on the markets, costs and purchase power, has worsened. While recognizing that much remains to be done to achieve the objective of the WSSD, the analyses indicate that actions implemented in the last decade under the CFP have led to an improvement in the status of many commercially important fish stocks and their fleets towards levels that are closer to those producing MSY.

Background In hot deserts daily/seasonal fluctuations pose great challenges to the resident organisms. However, these extreme ecosystems host unique microenvironments, such as the rhizosheath–root system of desert speargrasses in which biological activities and interactions are facilitated by milder conditions and reduced fluctuations. Here, we examined the bacterial microbiota associated with this structure and its surrounding sand in the desert speargrass $\textit{Stipagrostis pungens}$ under the contrasting environmental conditions of summer and winter in the Sahara Desert. Results The belowground rhizosheath–root system has higher nutrient and humidity contents, and cooler temperatures than the surrounding sand. The plant responds to the harsh environmental conditions of the summer by increasing the abundance and diversity of extracellular polymeric substances (EPS) compared to the winter. On the contrary, the bacterial community associated with the rhizosheath–root system and its interactome remain stable and, unlike the bulk sand, are unaffected by the seasonal environmental variations. The rhizosheath–root system bacterial communities are consistently dominated by Actinobacteria and $\textit{Alphaproteobacteria}$ and form distinct bacteria communities from those of bulk sand in the two seasons. The microbiome-stabilization mediated by the plant host acts to consistently retain beneficial bacteria with multiple plant growth promoting functions, including those capable to produce EPS, which increase the sand water holding capacity ameliorating the rhizosheath micro-environment. Conclusions Our results reveal the capability of plants in desert ecosystems to stabilize their below ground microbial community under seasonal contrasting environmental conditions, minimizing the heterogeneity of the surrounding bulk sand and contributing to the overall holobiont resilience under poly-extreme conditions. ; This research was supported by the EU project BIODESERT (European Community's Seventh Framework Programme CSA-SA REGPOT-2008–2 under grant agreement no. 245746), King Abdullah University of Science and Technology through the baseline research funds to DD, European Union's Horizon 2020 research and innovation programme under the Marie Sklodowska-Curie grant agreement No 841317 with the project "SENSE" for the funding support to ER and SB.

WKNSMSE (Workshop on North Sea stocks Management Strategy Evaluation) took place over two physical meetings (19-21 November 2018 and 26-28 February 2019, but at ICES HQ, Copenhagen) and several WebEx meetings, was chaired by José De Oliveira (UK) and included 30 participants from Denmark, Germany, Netherlands, Norway, Sweden, UK and the European Commission, and two reviewers from South African and New Zealand. The purpose of this process was to evaluate long-term management strategies for jointly-managed stocks in the North Sea (cod, haddock, whiting, saithe and autumn-spawning herring) between the European Union and Norway, following a request from EU-Norway. The first physical meeting provided an ICES interpretation of the EU-Norway request, agreed the specifications of the MSE, decided on the tools and approaches to use, and developed a work plan, while the second meeting (and subsequent follow-up WebEx meetings) discussed results, developed conclusions, ensured the minimum requirements for conducting MSEs (developed by WKGMSE2) were met, and finalised the report. ICES were tasked to find "optimal" combinations of harvest control rule parameters (Ftarget and Btrigger) for management strategies with or without stability mechanisms (TAC constraints and banking and borrowing scenarios). "Optimal" combinations were defined as those combinations of Ftarget and Btrigger that simultaneously maximised long-term yield while being precautionary (long-term risk3≤5%). The request also asked for sensitivity tests once the management strategies were "optimised". The approach adopted for all stocks was to include the assessment and forecast in a full-feedback MSE simulation, and to condition the baseline operating model on the benchmarked ICES assessment. The one exception was haddock, where it was not possible to include TSA in the full-feedback simulation because it was too slow to converge and requires manual intervention; SAM was used instead as a reasonable approximation. The approach also considered alternative operating models to capture a broader range of uncertainties. Full-feedback simulations were computationally challenging and required the use of parallelisation and high-performance computing; it also meant that the time-frame for the work was extremely tight, and in some cases, analyses were restricted. Nonetheless, the work was completed for all stocks, and "optimal" combinations for most management strategies were found. There were some notable issues that arose through this suite of MSEs, including that some management strategies that were precautionary in the long-term could have unsavoury and avoidable features in the short term (depending on the management strategy), and that reference points estimated by EqSim were, in many cases, no longer found to be precautionary in the MSE.

International audience ; Independence of science and best available science are fundamental pillars of the UN-FAO code of conduct 41 for responsible fisheries and are also applied to the European Union (EU) Common Fishery Policy (CFP), 42 with the overarching objective being the sustainable exploitation of the fisheries resources. CFP is 43 developed by DG MARE, the department of the European Commission responsible for EU policy on 44 maritime affairs and fisheries, which has the Scientific, Technical and Economic Committee for Fisheries 45 (STECF) as consultant body. In the Mediterranean and Black Sea, the General Fisheries Commission for the 46 Mediterranean (FAO-GFCM), with its own Scientific Advisory Committee on Fisheries (GFCM-SAC), plays a 47 critical role in fisheries governance, having the authority to adopt binding recommendations for fisheries 48 conservation and management. During the last years, advice on the status of the main stocks in the 49 Mediterranean and Black Sea has been provided both by GFCM-SAC and EU-STECF, often without a clear 50coordination and a lack of shared rules and practices. This has led in the past to: i) duplications of the 51 advice on the status of the stocks thus adding confusion in the management process and, ii) a continuous 52 managers' interference in the scientific process by DG MARE officials hindering its transparency and 53 independence. Thus, it is imperative that this stalemate is rapidly resolved and that the free role of science 54 in Mediterranean fisheries assessment and management is urgently restored to assure the sustainable 55 exploitation of Mediterranean marine resources in the future.

International audience ; Independence of science and best available science are fundamental pillars of the UN-FAO code of conduct 41 for responsible fisheries and are also applied to the European Union (EU) Common Fishery Policy (CFP), 42 with the overarching objective being the sustainable exploitation of the fisheries resources. CFP is 43 developed by DG MARE, the department of the European Commission responsible for EU policy on 44 maritime affairs and fisheries, which has the Scientific, Technical and Economic Committee for Fisheries 45 (STECF) as consultant body. In the Mediterranean and Black Sea, the General Fisheries Commission for the 46 Mediterranean (FAO-GFCM), with its own Scientific Advisory Committee on Fisheries (GFCM-SAC), plays a 47 critical role in fisheries governance, having the authority to adopt binding recommendations for fisheries 48 conservation and management. During the last years, advice on the status of the main stocks in the 49 Mediterranean and Black Sea has been provided both by GFCM-SAC and EU-STECF, often without a clear 50coordination and a lack of shared rules and practices. This has led in the past to: i) duplications of the 51 advice on the status of the stocks thus adding confusion in the management process and, ii) a continuous 52 managers' interference in the scientific process by DG MARE officials hindering its transparency and 53 independence. Thus, it is imperative that this stalemate is rapidly resolved and that the free role of science 54 in Mediterranean fisheries assessment and management is urgently restored to assure the sustainable 55 exploitation of Mediterranean marine resources in the future.

International audience ; Independence of science and best available science are fundamental pillars of the UN-FAO code of conduct 41 for responsible fisheries and are also applied to the European Union (EU) Common Fishery Policy (CFP), 42 with the overarching objective being the sustainable exploitation of the fisheries resources. CFP is 43 developed by DG MARE, the department of the European Commission responsible for EU policy on 44 maritime affairs and fisheries, which has the Scientific, Technical and Economic Committee for Fisheries 45 (STECF) as consultant body. In the Mediterranean and Black Sea, the General Fisheries Commission for the 46 Mediterranean (FAO-GFCM), with its own Scientific Advisory Committee on Fisheries (GFCM-SAC), plays a 47 critical role in fisheries governance, having the authority to adopt binding recommendations for fisheries 48 conservation and management. During the last years, advice on the status of the main stocks in the 49 Mediterranean and Black Sea has been provided both by GFCM-SAC and EU-STECF, often without a clear 50coordination and a lack of shared rules and practices. This has led in the past to: i) duplications of the 51 advice on the status of the stocks thus adding confusion in the management process and, ii) a continuous 52 managers' interference in the scientific process by DG MARE officials hindering its transparency and 53 independence. Thus, it is imperative that this stalemate is rapidly resolved and that the free role of science 54 in Mediterranean fisheries assessment and management is urgently restored to assure the sustainable 55 exploitation of Mediterranean marine resources in the future.