Suchergebnisse

Werden wir alternative Energiequellen erschließen und so die Klimakatastrophe abwenden können? Werden unsere Demokratien die Herausforderungen durch neue Kommunikationsformen und künstliche Intelligenz bestehen? Werden wir eine alternde Gesellschaft medizinisch versorgen können? Auf der Suche nach Antworten auf diese und viele weitere "Zukunftsfragen" besuchte Patrick Cramer die 84 Institute der Max-Planck-Gesellschaft, bevor er deren Vorsitz antrat. Dabei begegnete er leidenschaftlich forschenden Wissenschaftlerinnen und Wissenschaftlern und lernte Zukunftswelten kennen, die gerade erst in ihren Köpfen entstehen. Von Astronomie und Klimaforschung über Biomedizin und künstliche Intelligenz bis hin zu Energieforschung und Gesellschaftswissenschaften - Cramer nimmt uns mit in die Maschinenräume der Forschung und gewährt exklusive Einblicke in die Wissenschaft von morgen

Gene expression in eukaryotes does not follow a linear process from transcription to translation and mRNA degradation. Instead it follows a circular process in which cytoplasmic mRNA decay crosstalks with nuclear transcription. In many instances, this crosstalk contributes to buffer mRNA at a roughly constant concentration. Whether the mRNA buffering concept operates on the total mRNA concentration or at the gene-specific level, and if the mechanism to do so is a global or a specific one, remain unknown. Here we assessed changes in mRNA concentrations and their synthesis rates along the transcriptome of aneuploid strains of the yeast Saccharomyces cerevisiae. We also assessed mRNA concentrations and their synthesis rates in nonsense-mediated decay (NMD) targets in euploid strains. We found that the altered synthesis rates in the genes from the aneuploid chromosome and the changes in their mRNA stabilities were not counterbalanced. In addition, the stability of NMD targets was not specifically compensated by the changes in synthesis rate. We conclude that there is no genetic compensation of NMD mRNA targets in yeast, and total mRNA buffering uses mostly a global system rather than a gene-specific one. ; This work was funded with grants from the Spanish Ministry of Economy and Competitiveness, the European Union (FEDER) [BFU2016-77728-C3-1-P to S. C.], [BFU2016-77728-C3-3-P to J.E.P -O] and [RED2018-102467-T to J.E.P -O and S.C.], the Regional Valencian Government [AICO2019/088 to J.E.P-O] and the Junta de Andalucía [BIO-271 to S.C.]. ; No

Gene expression in eukaryotes does not follow a linear process from transcription to translation and mRNA degradation. Instead it follows a circular process in which cytoplasmic mRNA decay crosstalks with nuclear transcription. In many instances, this crosstalk contributes to buffer mRNA at a roughly constant concentration. Whether the mRNA buffering concept operates on the total mRNA concentration or at the gene-specific level, and if the mechanism to do so is a global or a specific one, remain unknown. Here we assessed changes in mRNA concentrations and their synthesis rates along the transcriptome of aneuploid strains of the yeast Saccharomyces cerevisiae. We also assessed mRNA concentrations and their synthesis rates in nonsense-mediated decay (NMD) targets in euploid strains. We found that the altered synthesis rates in the genes from the aneuploid chromosome and the changes in their mRNA stabilities were not counterbalanced. In addition, the stability of NMD targets was not specifically compensated by the changes in synthesis rate. We conclude that there is no genetic compensation of NMD mRNA targets in yeast, and total mRNA buffering uses mostly a global system rather than a gene-specific one. ; This work was funded with grants from the Spanish Ministry of Economy and Competitiveness, the European Union (FEDER) [BFU2016-77728-C3-1-P to S. C.], [BFU2016-77728-C3-3-P to J.E.P -O] and [RED2018-102467-T to J.E.P -O and S.C.], the Regional Valencian Government [AICO2019/088 to J.E.P-O] and the Junta de Andalucía [BIO-271 to S.C.].