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Intro -- Title -- Copyright -- Contents -- Introduction -- Part 1: Reimagining Australia -- Time to heal: Uluru healing the people and the land -- It's possible -- How to start: Processes for discerning post-viral strategy -- An emissions and employment accord -- The health and wellbeing of future generations -- To surge forward, not snap back -- Part 2: A framework for the future -- A modern framework for thinking about debt and deficits -- Towards an affirmational republic -- Rethinking government: Lessons from the National Cabinet -- Fairness and sustainability through population policy -- The business of building back better -- Working together for a better Australia -- Reducing inequality through the reconstruction -- Part 3: Policy settings for fairness and sustainability -- Infrastructure and investment for the reconstruction -- Sustainable agriculture and food production -- Water security for rural and regional Australia -- Renewable energy to power the reconstruction -- Making it in Australia -- Building opportunity through economic complexity -- The case for stump-jump policy -- Rebuilding from the ground up: the role of the foundational economy -- Part 4: Essential services for a stronger Australia -- Reimagining public health in Australia -- Rebuilding the public sector -- A social guarantee -- Reimagining school education -- Rebuilding a sustainable and fair tertiary education system for Australia -- Reforming employment services for the reconstruction -- Four lessons from the great disruption -- Conclusion -- Acknowledgements -- Contributors -- Notes -- Index.

Abstract
Phenotypic heterogeneity is an important mechanism for regulating bacterial virulence, where a single regulatory switch is typically activated to generate virulent and avirulent subpopulations. The opportunistic pathogen Acinetobacter baumannii can transition at high frequency between virulent opaque (VIR-O) and avirulent translucent subpopulations, distinguished by cells that form opaque or translucent colonies. We demonstrate that expression of 11 TetR-type transcriptional regulators (TTTRs) can drive cells from the VIR-O opaque subpopulation to cells that form translucent colonies. Remarkably, in a subpopulation of VIR-O cells, four of these TTTRs were stochastically activated in different combinations to drive cells to the translucent state. The resulting translucent subvariants exhibited unique phenotypic differences and the majority were avirulent. Due to their functional redundancy, a quadruple mutant with all four of these TTTRs inactivated was required to observe a loss of switching from the VIR-O state. Further, we demonstrate a small RNA, SrvS, acts as a "rheostat," where the levels of SrvS expression influences both the VIR-O to translucent switching frequency, and which TTTR is activated when VIR-O cells switch. In summary, this work has revealed a new paradigm for phenotypic switching in bacteria, where an unprecedented number of related transcriptional regulators are activated in different combinations to control virulence and generate unique translucent subvariants with distinct phenotypic properties.