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Cover -- Contents -- Preface -- List of Contributors -- 1 Current Challenges in Introducing Beneficial Microorganisms into the Rhizosphere -- 2 Studies on Indigenous Endophytic Bacteria of Sweet Corn and Cotton -- 3 Detection of Introduced Bacteria in the Rhizosphere Using Marker Genes and DNA Probes -- 4 Impact of GEMMOs on Rhizosphere Population Dynamics -- 5 Developing Concepts in Biological Control: A Molecular Ecology Approach -- 6 Biocontrol of Root Diseases by Pseudomonas fluorescens CHAO: Current Concepts and Experimental Approaches -- 7 Genetic Strategies to Engineer Expression Systems Responsive to Relevant Environmental Signals -- 8 Genetic Qping of Microorganisms: Current Concepts and Future Prospects -- 9 Development of Subtraction Hybridization Procedures for Generating Strain-Specific Rhizobium DNA Probes -- 10 Molecular Characterization and Detection of the Actinomycete Jkunkiu in the Environment -- 11 Molecular Ecology of Filamentous Actinomycetes in Soil -- 12 Some Considerations on Gene Transfer between Bacteria in Soil and Rhizophere -- 13 European Community Regulation for the Use and Release of Genetically Modified Organisms (GMOs) in the Environment -- Index.

peer-reviewed ; Gaining a greater understanding of the plant microbiota and its interactions with its host plant heralds a new era of scientific discovery in agriculture. Different agricultural management practices influence soil microbial populations by changing a soil's physical, chemical and biological properties. However, the impact of these practices on the microbiota associated with economically important crops such as oilseed rape, are still understudied. In this work we investigated the impact of two contrasting crop establishment practices, conventional (plow based) and conservation (strip–tillage) systems, on the microbiota inhabiting different plant microhabitats, namely rhizosphere, root and shoot, of winter oilseed rape under Irish agronomic conditions. Illumina 16S rRNA gene sequence profiling showed that the plant associated microhabitats (root and shoot), are dominated by members of the bacterial phyla Proteobacteria, Actinobacteria and Bacteroidetes. The root and shoot associated bacterial communities displayed markedly distinct profiles as a result of tillage practices. We observed a very limited 'rhizosphere effect' in the root zone of WOSR, i.e., there was little or no increase in bacterial community richness and abundance in the WOSR rhizosphere compared to the bulk soil. The two tillage systems investigated did not appear to lead to any major long term differences on the bulk soil or rhizosphere bacterial communities. Our data suggests that the WOSR root and shoot microbiota can be impacted by management practices and is an important mechanism that could allow us to understand how plants respond to different management practices and environments. ; This work was supported through the Teagasc Walsh Fellowship Scheme which funded RR. The analysis of the sequencing data was supported by Royal Society of Edinburgh/Scottish Government Personal Research Fellowship co-funded by Marie Curie Actions awarded to DB.