Suchergebnisse

[EN] CuII-macrocycle functionalized hexametaphosphate-capped silica mesoporous nanoparticles have been prepared and used for the selective and sensitive detection of hydrogen sulfide in aqueous environments. The possibility of using different metal complexes combined with different capping anions and choice of different dyes or other sensing molecules as indicators makes this new protocol highly appealing for the preparation of new sensing systems for sulfide detection in different environments. ; Financial support from the Spanish Government (Project MAT2012-38429-C04-01) and the Generalitat Valencia (Project PROMETEOII/2014/047) is gratefully acknowledged. S.E. is grateful to the Generalitat Valenciana for his Santiago Grisolia fellow. ; El Sayed Shehata Nasr, S.; Milani, M.; Licchelli, M.; Martínez-Máñez, R.; Sancenón Galarza, F. (2015). Hexametaphosphate-Capped Silica Mesoporous Nanoparticles Containing CuII Complexes for the Selective and Sensitive Optical Detection of Hydrogen Sulfide in Water. Chemistry - A European Journal. 21(19):7002-7006. https://doi.org/10.1002/chem.201500360 ; S ; 7002 ; 7006 ; 21 ; 19

[EN] Two different acetylcholinesterase (AChE)-capped mesoporous silica nanoparticles (MSNs), S1-AChE and S2-AChE, were prepared and characterized. MSNs were loaded with rhodamine B and the external surface was functionalized with either pyridostigmine derivative P1 (to yield solid S1) or neostigmine derivative P2 (to obtain S2). The final capped materials were obtained by coordinating grafted P1 or P2 with AChE ' s active sites (to give S1-AChE and S2-AChE, respectively). Both materials were able to release rho-damine B in the presence of diisopropylfluorophosphate (DFP) or neostigmine in a concentration-dependent manner via the competitive displacement of AChE through DFP and neostigmine coordination with the AChE ' s active sites. The responses of S1-AChE and S2-AChE were also tested with other enzyme inhibitors and substrates. These studies suggest that S1-AChE nanoparticles can be used for the selective detection of nerve agent simulant DFP and paraoxon. ; Financial support from the Spanish Government and FEDER funds (Project MAT2015‐64139‐C4‐1‐R, AGL2015‐70235‐C2‐2‐R) and the Generalitat Valencia (Project PROMETEOII/2014/047) is gratefully acknowledged. Ll. P. is grateful to the Universitat Politécnica de Valencia for his grant. ; Pascual, L.; El Sayed Shehata Nasr, S.; Marcos Martínez, MD.; Martínez-Máñez, R.; Sancenón Galarza, F. (2017). Acetylcholinesterase-capped Mesoporous Silica Nanoparticles Controlled by the Presence of Inhibitors. Chemistry - An Asian Journal. 12(7):775-784. https://doi.org/10.1002/asia.201700031 ; S ; 775 ; 784 ; 12 ; 7 ; Alberti, S., Soler-Illia, G. J. A. A., & Azzaroni, O. (2015). Gated supramolecular chemistry in hybrid mesoporous silica nanoarchitectures: controlled delivery and molecular transport in response to chemical, physical and biological stimuli. Chemical Communications, 51(28), 6050-6075. doi:10.1039/c4cc10414e ; Aznar, E., Oroval, M., Pascual, L., Murguía, J. R., Martínez-Máñez, R., & Sancenón, F. (2016). Gated Materials for On-Command Release of Guest ...

Three different mesoporous silica nano-sized materials (SC1, SC2, and SC3), loaded with [Ru(bipy)(3)](2+) dye (bipy= bipyridine) and functionalized on the external surface with three macrocyclic copper(II) complexes (C1, C2, and C3), were synthesized and characterized. When SC1, SC2, and SC3 were suspended in water, the entrapped [Ru(bipy)(3)](2+) dye was free to diffuse from the inner pores to the solution. However, addition of anions induced certain degrees of pore blockage, with subsequent dye release inhibition. Small monovalent and divalent anions were unable to induce complete pore blockage, whereas bulky and highly charged anions induced marked reductions in [Ru(bipy)(3)](2+) delivery. The best [Ru(bipy)(3)](2+) delivery inhibitors were ATP and hexametaphosphate anions. Inhibition was ascribed to the interaction of the anions with the grafted Cu-II complexes on the surface of the SC1, SC2, and SC3 supports. The hexametaphosphate anion was selected to prepare two capped materials (SC1-mPh and SC3-mPh). Studies of the [Ru(bipy)(3)](2+) dye release from solids SC1-mPh and SC3-mPh alone and in the presence of a collection of selected anions (HS-, F-, Br-, Cl-, I-, CN-, HPO42-, AcO-, citrate, NO32-, HCO3-, SO42-, and S2O82-), amino acids (alanine and histidine), thiol-containing biomolecules (cysteine, methylcysteine, homocysteine, and glutathione (GSH)), and oxidants (H2O2) were performed. None of the chemicals tested, except hydrogen sulphide, was able to induce remarkable cargo delivery in both solids. The observed dye release was ascribed to a demetalation reaction of the C1 and C3 complexes induced by the hydrogen sulphide anion. ; Financial support from the Spanish Government (Project MAT2012-38429-C04-01) and the Generalitat Valencia (Project PROMETEO/2009/016) is gratefully acknowledged. ; El Sayed Shehata Nasr, S.; Milani, M.; Milanese, C.; Licchelli, M.; Martínez-Máñez, R.; Sancenón Galarza, F. (2016). Anions as Triggers in Controlled Release Protocols from Mesoporous Silica Nanoparticles ...

[EN] Mesoporous silica nanoparticles loaded with safranin O and capped with disulfide-containing oligo(ethylene glycol) chains were used for the selective and sensitive fluorimetric detection of glutathione. ; Financial support from the Spanish Government (Project MAT2012-38429-C04-01) and the Generalitat Valencia (Project PROMETEOII/2014/047) is gratefully acknowledged. S. E. is grateful to the Generalitat Valenciana for his Santiago Grisolia fellow. Also, C. G. is grateful to the Spanish Ministry of Science and Innovation for the grant. ; El Sayed Shehata Nasr, S.; Giménez Morales, C.; Aznar Gimeno, E.; Martínez Mañez, R.; Sancenón Galarza, F.; Licchelli, M. (2015). Highly selective and sensitive detection of glutathione using mesoporous silica nanoparticles capped with disulfide-containing oligo(ethylene glycol) chains. Organic and Biomolecular Chemistry. 13(4):1017-1021. https://doi.org/10.1039/C4OB02083A ; S ; 1017 ; 1021 ; 13 ; 4

[EN] The consumption of illicit drugs has increased exponentially in recent years and has become a problem that worries both governments and international institutions. The rapid emergence of new compounds, their easy access, the low levels at which these substances are able to produce an effect, and their short time of permanence in the organism make it necessary to develop highly rapid, easy, sensitive, and selective methods for their detection. Currently, the most widely used methods for drug detection are based on techniques that require large measurement times, the use of sophisticated equipment, and qualified personnel. Chromo- and fluorogenic methods are an alternative to those classical procedures. ; We thank the Spanish Government [projects MAT2015-64139-C4-1-R and AGL2015-70235-C2-2-R (MINECO/FEDER)] and the Generalitat Valenciana (project PROMETEOII/2014/047) for support. S.E.S thanks the Ministerio de Economia y Competitividad for his Juan de la Cierva contract. B.L.T and E.G. thank the Spanish Government for their predoctoral grants. L.P. also thanks the Universitat Politecnica de Valencia for his predoctoral grant. ; Garrido-García, EM.; Pla, L.; Lozano-Torres, B.; El Sayed Shehata Nasr, S.; Martínez-Máñez, R.; Sancenón Galarza, F. (2018). Chromogenic and Fluorogenic Probes for the Detection of Illicit Drugs. ChemistryOpen. 7(5):401-428. https://doi.org/10.1002/open.201800034 ; S ; 401 ; 428 ; 7 ; 5 ; Komoroski, E. M., Komoroski, R. A., Valentine, J. L., Pearce, J. M., & Kearns, G. L. (2000). The Use of Nuclear Magnetic Resonance Spectroscopy in the Detection of Drug Intoxication. Journal of Analytical Toxicology, 24(3), 180-187. doi:10.1093/jat/24.3.180 ; Drugs of Abuse: A DEA Resource Guide 2017 ; World Drug Report 2017 ; European Drug Report: Trends and Developments 2017 ; Namera, A., Nakamoto, A., Saito, T., & Nagao, M. (2011). Colorimetric detection and chromatographic analyses of designer drugs in biological materials: a comprehensive review. Forensic Toxicology, 29(1), 1-24. ...

[EN] Trivalent cations (Al3+, Fe3+, Cr3+, As3+, In3+ and Ga3+) induced a dehydration reaction of a chemodosimeter in water that is coupled with colour and emission changes. ; Financial support from the Spanish Government and FEDER funds (Project MAT2015-64139-C4-1) and the Generalitat Valencia (Project PROMETEO II/2014/047) is gratefully acknowledged. M. L. P. is grateful to the Generalitat Valenciana for her Santiago Grisolia grant. ; Lo-Presti, M.; El Sayed Shehata Nasr, S.; Martínez-Máñez, R.; Costero, AM.; Gil, S.; Parra, M.; Sancenón Galarza, F. (2016). Selective chromo-fluorogenic detection of trivalent cations in aqueous environments using a dehydration reaction. New Journal of Chemistry. 40(11):9042-9045. https://doi.org/10.1039/c6nj01957a ; S ; 9042 ; 9045 ; 40 ; 11

The synthesis, characterization and sensing features of a novel probe 1 for the selective chromogenic recognition of diisopropylfluorophosphate (DFP), a sarin and soman mimic, in 99:1 (v/ v) water/acetonitrile and in the gas phase is reported. Colour modulation is based on the combined reaction of phosphorylation of 1 and fluoride-induced hydrolysis of a silyl ether moiety. As fluoride is a specific reaction product of the reaction between DFP and the OH group, the probe shows a selective colour modulation in the presence of this chemical. Other nerve agent simulants, certain anions, oxidant species and other organophosphorous compounds were unable to induce colour changes in 1. This is one of the very few examples of a selective detection, in solution and in the gas phase, of a sarin and soman simulant versus other reactive derivatives such as the tabun mimic diethylcyanophosphate (DCNP). ; Financial support from the Spanish Government (Project MAT2012-38429-C04) is gratefully acknowledged. S. E. is grateful to the Generalitat Valenciana for his Santiago Grisolia fellowship. L. P. thanks the Universitat Politecnica de Valencia for his doctoral fellowship. ; El Sayed Shehata Nasr, S.; Pascual Vidal, L.; Agostini, A.; Martínez-Máñez, R.; Sancenón Galarza, F.; Costero Nieto, AM.; Parra Álvarez, M. (2014). A Chromogenic Probe for the Selective Recognition of Sarin and Soman Mimic DFP. ChemistryOpen. 3(4):142-145. https://doi.org/10.1002/open.201402014 ; S ; 142 ; 145 ; 3 ; 4

[EN] Four probes (i.e. D1¿D4) for the selective and sensitive fluorogenic detection of HS2 have been prepared and characterised. HEPES (10 mM, pH 7.4)¿DMSO 99:1 v/v solutions of D1¿D4 are essentially non-fluorescent. Changes in the emission using D1¿D4 in the presence of anions (F2, Cl2, Br2, I2, N2 3 , CN2, SCN2, AcO2, CO22 3 , PO22 4 , SO22 4 , HS2 and OH2), biothiols (GSH, Cys, Hcy, Me ¿Cys and lipoic acid), reducing agents (SO22 3 and S2O22 3 ) and oxidants (H2O2) demonstrated that only HS2 is able to induce the appearance of intense emission bands in the 400¿ 520 nm range in the four probes. The selectivity observed was ascribed to a unique hydrogen sulfide-induced hydrolysis of the 2,4-dinitrophenyl ether moiety that yielded the corresponding free highly fluorescent alcohols. The potential detection of intracellular HS2 was also studied. ; Financial support from the Spanish Government (Project MAT2012-38429-C04-01) and the Generalitat Valencia (Project PROMETEO/2009/016) is gratefully acknowledged. S.E. is grateful to the Generalitat Valenciana for his Santiago Grisolia fellow. L.E.S.F. also thanks the Carolina Foundation and UPNFM-Honduras for his doctoral grant. ; El Sayed Shehata Nasr, S.; De La Torre, C.; Santos Figueroa, LE.; Martínez-Máñez, R.; Sancenón Galarza, F.; Orzáez, M.; Costero, AM. (2015). 2,4-dinitrophenyl ether-containing chemodosimeters for the selective and sensitive 'in vitro' and 'in vivo' detection of hydrogen sulfide. Supramolecular Chemistry. 27(4):244-254. https://doi.org/10.1080/10610278.2014.977286 ; S ; 244 ; 254 ; 27 ; 4

[EN] Three fluorescent probes (1–3) for the selective and sensitive detection of hydrogen sulfide have been synthesized and characterized. Probe 1 is a coumarin derivative functionalized with an azide moiety whereas 2 contain the azide reactive group into a naphthalene fluorophore backbone. Probe 3 is composed also by a naphthalene fluorophore but, in this case, functionalized with a sulfonylazide reactive moiety. Probes 1 and 3 are non-fluorescent whereas 2 is weakly emissive in HEPES (10 mM, pH 7.4)–DMSO 99:1 (v/v). The emission behavior of the three probes was tested against selected anions, bio-thiols and oxidant molecules. Of all the chemical species tested, only HS− is able to induce an enhancement in the emission intensity (50, 11 and 20-fold for 1, 2 and 3, respectively). The observed emission in the presence of hydrogen sulfide is ascribed, in the case of probes 1 and 2, to an azide–amine reduction induced by HS− anion, whereas for probe 3 the sensing mechanism is related with a sulfonylazide–sulfonamide conversion. The three probes are very sensitive to HS− anion with limits of detection of 0.17, 0.20 and 0.40 mM for 1, 2 and 3 respectively. Cell viability studies demonstrated that 1–3 probes are essentially non-toxic at concentrations 10–50 μM and are well suited for in vivo studies. Finally, probe 1 was used for the detection on intracellular HS− anion in HeLa cells by means of confocal microscopy. ; Financial support from the Spanish Government (Project MAT2012-38429-004-01) and the Generalitat Valenciana (Project PROMETEO/2009/016) is gratefully acknowledged. S.E. is grateful to the Generalitat Valenciana for his Santiago Grisolia fellow. C.T. also thanks the Ministerio de Ciencia e Innovacion for her FPU grant. L.E.S.F. thanks the Carolina Foundation and UPNFM-Honduras for his doctoral grant. ; El Sayed Shehata Nasr, S.; De La Torre Paredes, C.; Santos Figueroa, LE.; Marín Hernández, C.; Martínez Mañez, R.; Sancenón Galarza, F.; Costero Nieto, AM. (2015). Azide and sulfonylazide functionalized ...