Suchergebnisse

Negli ultimi anni, in seguito ad un esponenziale aumento degli arrivi internazionali , il turismo urbano di massa e il diffondersi delle locazioni brevi mediate da Airbnb hanno innescato un rapido processo di turistificazione nel centro storico di Napoli. La tesi analizza gli impatti di questo fenomeno sollevando questioni urgenti in merito alle sue conseguenze in termini di aumento delle disuguaglianze, espulsione degli abitanti e mercificazione del patrimonio culturale. A partire dalla specificità del caso di studio e dal ruolo che la casa svolge all'interno del processo di turistificazione, la tesi analizza in particolare quella che definisce "la turistificazione del residenziale", interpretandola come una declinazione contemporanea della rendita urbana. In fine, propone un'analisi critica delle politiche pubbliche che influiscono sul fenomeno, suggerendo gli strumenti di pianificazione utili ad invertire la tendenza alla monocoltura turistifica.

Approaches used to design, build, and maintain digital cultural heritage communities and infrastructure in Europe, North America, and Australasia need to be tailored to regional contexts such as Middle East and North Africa (MENA). Cultural and political differences, inherited issues with technical infrastructure and funding, and the need to build trusting and healthy working relationships across national boundaries makes this challenging. The framework and roadmap used during the MaDiH (مديح): Mapping Digital Cultural Heritage in Jordan project (2019 - 2021) provides one of several possible models for such work, as well as highlighting its myriad challenges and opportunities.

This white paper reports on the findings of the MaDiH (مديح): Mapping Digital Cultural Heritage in Jordan project. It is complemented by a technical white paper. MaDiH (مديح) is a collaborative project between King's Digital Lab (KDL) at King's College London, the Hashemite University (HU), the Council for British Research in the Levant (CBRL), the Department of Antiquities of Jordan (DoA), the Jordan Open Source Association (JOSA), and the Endangered Archaeology in the Middle East and North Africa (EAMENA) project. The project ran for two years, from February 2019 - April 2021. The goal of the project was to contribute to the long-term sustainable development of Jordan's digital cultural heritage by identifying key systems, datasets, standards, and policies, and aligning them to government digital infrastructure capabilities and strategies. This policy white paper focuses on alignment to national and international policies and standards and makes recommendations for future action. The technical white paper provides more detailed technical descriptions of standards, datasets, data repositories, and other assets, and defines requirements for future activity. Each section of the white papers lists associated challenges and opportunities, which are summarised in appendices. Taken together, the intention is for the white papers to define the current state of digital cultural heritage (DCH) in Jordan, inform and align to Jordanian government policy, and inform planning and technical design processes that can enable the future development of Jordanian DCH. MaDiH's (مديح) policy goals aimed to ensure any technical work or analysis undertaken in the project were aligned to government policies and international best practices in cultural heritage management, digital cultural heritage, and research software engineering. Practical prototyping was used to ensure analysis and lessons learned are cost-effective and aligned to real-world scenarios, and a series of workshops with stakeholders from the cultural heritage, ...

This white paper reports on the findings of the MaDiH: Mapping Digital Cultural Heritage in Jordan project. It is complemented by a policy white paper. Its goal is to report on technical matters related to the project and provide advice and recommendations for future technical activity. We hope the paper is of interest to a broad audience, although it is primarily tailored to the Jordanian cultural heritage and technology communities. MaDiH (مديح) is a collaborative UK-Jordanian project between King's Digital Lab (KDL) at King's College London, the Hashemite University (HU), the Council for British Research in the Levant (CBRL), the Department of Antiquities of Jordan (DoA), the Jordanian Open Source Association (JOSA), and the Endangered Archaeology in the Middle East and North Africa (EAMENA) project. The project ran for just over two years, from February 2019 to April 2021. The goal of the project was to contribute to the long-term sustainable development of Jordan's digital cultural heritage by identifying key systems, datasets, standards, and policies, and aligning them to government digital infrastructure capabilities and strategies. This technical white paper describes the current state of standards, datasets, data repositories and other assets, and defines requirements for future activity. The MaDiH (مديح) Policy White Paper focuses on alignment to national and international policies and standards and makes recommendations for future action. Each section of the white papers lists associated challenges and recommendations, which are summarised in appendices. Taken together, the intention is for the white papers to define the current state of digital cultural heritage (DCH) in Jordan, inform and align to Jordanian government policy, and inform planning and technical design processes that can enable the future development of Jordanian DCH. MaDiH (مديح)'s technical goals focused on the definition of a robust technical and operational architecture for DCH to assist, among others, the Department of Antiquities ...

Batten diseases (BDs) are a group of lysosomal storage disorders characterized by seizure, visual loss, and cognitive and motor deterioration. We discovered increased levels of globotriaosylceramide (Gb3) in cellular and murine models of CLN3 and CLN7 diseases and used fluorescent-conjugated bacterial toxins to label Gb3 to develop a cell-based high content imaging (HCI) screening assay for the repurposing of FDA-approved compounds able to reduce this accumulation within BD cells. We found that tamoxifen reduced the lysosomal accumulation of Gb3 in CLN3 and CLN7 cell models, including neuronal progenitor cells (NPCs) from CLN7 patient-derived induced pluripotent stem cells (iPSC). Here, tamoxifen exerts its action through a mechanism that involves activation of the transcription factor EB (TFEB), a master gene of lysosomal function and autophagy. In vivo administration of tamoxifen to the CLN7Δex2 mouse model reduced the accumulation of Gb3 and SCMAS, decreased neuroinflammation, and improved motor coordination. These data strongly suggest that tamoxifen may be a suitable drug to treat some types of Batten disease. ; This work was funded by the European Union's Horizon 2020 research and innovation programme (BATCure, grant No. 666918 to DLM, JPB, SEM, TB and SS). JPB is funded by the Agencia Estatal de Investigación (PID2019-105699RB-I00/ AEI / 10.13039/501100011033 and RED2018-102576-T), Plan Nacional sobre Drogas (2020I028), Junta de Castilla y León (Escalera de Excelencia CLU-2017-03), Ayudas Equipos Investigación Biomedicina 2017 Fundación BBVA and Fundación Ramón Areces. SS was funded by a grant from the Mila's Miracle Foundation. TB was supported by German Research Council (DFG) grant FOR2625. SM benefits from MRC funding to the MRC Laboratory for Molecular Cell Biology University Unit at UCL (award code MC_U12266B) towards laboratory and office space. We acknowledge Marcella Cesana for providing the TFEB virus. Graphical abstract was created using BioRender.com.

Funding: We thank Liliana Bento for valuable assistance in formatting the manuscript. This article was supported by the LYSOCIL project. This project has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No 811087. CGA has funding from Maratona da Saúde 2016 and Fundaçao para a Ciência e a Tecnologia ˜ , I.P. (CEECIND/00410/2017). FMP is a Wellcome Investigator in Science and a Royal Society Wilson merit award holder. ; Since the discovery of lysosomes more than 70 years ago, much has been learned about the functions of these organelles. Lysosomes were regarded as exclusively degradative organelles, but more recent research has revealed that they play essential roles in several other cellular functions, such as nutrient sensing, intracellular signalling, and metabolism. Methodological advances played a key part in generating our current knowledge about the biology of this multifaceted organelle. In this review, we cover current methods used to analyse lysosome morphology, positioning, motility, and function. We highlight the principles behind these methods, the methodological strategies, and their advantages and limitations. To extract accurate information and avoid misinterpretations, we discuss the best strategies to identify lysosomes and assess their characteristics and functions. With this review, we aim to stimulate an increase in the quantity and quality of research on lysosomes and further ground-breaking discoveries on an organelle that continues to surprise and excite cell biologists. ; publishersversion ; published