Cryo-electron microscopy of enveloped viruses using upgraded transmission electron microscope: Influenza type A, B viruses and SARS-CoV-2
In: Vestnik Moskovskogo universiteta. Seria 16. Biologia, Band 78, Heft №3s, 2023, S. 21-26
Cryo-electron microscopy (cryo-EM) is indispensable for the structural studies of enveloped
viruses – dangerous pathogens of humans and animals. Yet, it requires highly specialized
equipment as well as careful sample preparation. In this work, the capabilities of transmission
electron microscope JEOL JEM-2100 equipped with cryo-transfer holder are used, and
preliminary cryo-EM data for influenza A and B virus strains and SARS-CoV-2 inactivated with
beta-propiolactone are presented. Image analysis allows: (1) to distinguish "empty" viral
particles from "full" ones (containing nucleocapsid); (2) to visualize the lipid bilayer of the viral
envelope; (3) identify influenza virus surface antigens and the M1 protein layer combined with
the inner lipid monolayer; (4) distinguish different morphology of S-spikes on the surface of
inactivated SARS-CoV-2 virions. The developed approach provides good image quality for both
fundamental and applied research.