Suchergebnisse

The Grand Guignol of Paris was a theater company specialized in representing shortplays, the most recurring genre being that of terror, with works plagued by sadis m,cruelty and exacerbated violence. From the very beginning, the Grand Guignol hadproblems with censorship, in the form of impediments to grant the so called visas orauthorizations that allowed the representation of the different shows. This articleexa mines different cases of censorship to which the Gr and Guignol was submitted, theway in which they were evaded, the effect it produced on the company's progress, andthe way in which such censorship cases served as a reflection of the socio politicalcont ext in the Paris of the time. ; El Grand Guignol de Paris fue una compañía teatral que se especializó en representarobras de teatro breve , siendo el género más recurrente el del terror , con obras plagadasd e sadismo, crueldad y violencia exacerbada. Desde su mismo inicio, el Grand Guignoltuvo problemas con la censura, en forma de impedimentos para conceder las llamadasvisas o autorizaciones que permitían la representación de los distintos espectáculos. Seexamina en este artículo distintos casos de censura a que fue sometido el GrandGuignol, la forma en que fueron eludi dos, el efecto que produjo en la marcha de lacompañía y el modo en que dichos casos de censura sirvieron como reflejo del ambientesociopo lítico en el París de la época.

Cancer hereditario de mama y ovario; Pseudoexones; Variantes intrónicas profundas spliceogénicas ; Càncer hereditari de mama i d'ovari; Pseudoexons; Variants intròniques profundes spliceogèniques ; Hereditary breast ovarian cancer; Pseudoexons; Spliceogenic deep intronic variants ; The contribution of deep intronic splice-altering variants to hereditary breast and ovarian cancer (HBOC) is unknown. Current computational in silico tools to predict spliceogenic variants leading to pseudoexons have limited efficiency. We assessed the performance of the SpliceAI tool combined with ESRseq scores to identify spliceogenic deep intronic variants by affecting cryptic sites or splicing regulatory elements (SREs) using literature and experimental datasets. Our results with 233 published deep intronic variants showed that SpliceAI, with a 0.05 threshold, predicts spliceogenic deep intronic variants affecting cryptic splice sites, but is less effective in detecting those affecting SREs. Next, we characterized the SRE profiles using ESRseq, showing that pseudoexons are significantly enriched in SRE-enhancers compared to adjacent intronic regions. Although the combination of SpliceAI with ESRseq scores (considering ∆ESRseq and SRE landscape) showed higher sensitivity, the global performance did not improve because of the higher number of false positives. The combination of both tools was tested in a tumor RNA dataset with 207 intronic variants disrupting splicing, showing a sensitivity of 86%. Following the pipeline, five spliceogenic deep intronic variants were experimentally identified from 33 variants in HBOC genes. Overall, our results provide a framework to detect deep intronic variants disrupting splicing. ; This research was funded by the Spanish Instituto de Salud Carlos III (ISCIII) funding an initiative of the Spanish Ministry of Economy and Innovation, partially supported by European Regional Development FEDER Funds, grant numbers PI16/01218 and PI19/01303. AM-F contract is supported by the award ERAPERMED2019-215 granted by AECC FC and by ISCIII thorough AES 2019, both within the ERAPerMed framework". J.D.-V. contract is supported by the Secretariat for Universities and Research of the Ministry of Business and Knowledge of the Government of Catalonia and the European Social Fund.