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Organización del espacio y economía rural en la España Atlántica
In: Economía y demografía
The region and Spain's regional changes ; La región y los cambios regionales en España
In fifties, region was conceptualized in Spain as an individualized landscape. Anyway, such a formulation, when their deficiencies were being surmounting, was elided in historical pseudo-regions by political interests. Nowadays, with the important changes experimented in Spain, the author exposes what a region could be in a geographical meaning and he points the guidelines to get away with it. ; La región tuvo una conceptuación en España en los años cincuenta, como un paisaje individualizado. Sin embargo, tal formulación, cuando se estaba superando sus deficiencias, fue elidida por intereses políticos por unas pseudo regiones históricas. En los momentos actuales con los importantes cambios experimentados por España, se expone, que es lo que puede ser una región en el sentido geográfico, y se señalan las directrices para lograrlo.
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La región y los cambios regionales en España ; The region and Spain's regional changes
Conferencia leída el 18 de noviembre de 1998 en el Instituto Universitario de Geografía de la Universidad de Alicante ; La región tuvo una conceptuación en España en los años cincuenta, como un paisaje individualizado. Sin embargo, tal formulación, cuando se estaba superando sus deficiencias, fue elidida por intereses políticos por unas pseudo regiones históricas. En los momentos actuales con los importantes cambios experimentados por España, se expone, que es lo que puede ser una región en el sentido geográfico, y se señalan las directrices para lograrlo. ; In fifties, region was conceptualized in Spain as an individualized landscape. Anyway, such a formulation, when their deficiencies were being surmounting, was elided in historical pseudo-regions by political interests. Nowadays, with the important changes experimented in Spain, the author exposes what a region could be in a geographical meaning and he points the guidelines to get away with it.
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Complete functional characterization of disease-associated genetic variants in the complement factor H gene
30 p.-7 fig. ; Genetic analyses in atypical hemolytic uremic syndrome (aHUS) and C3-glomerulopathy (C3G) patients have provided an excellent understanding of the genetic component of the disease and informed genotype-phenotype correlations supporting an individualized approach to patient management and treatment. In this context, a correct categorization of the disease-associated gene variants is critical to avoid detrimental consequences for patients and their relatives. Here we describe a comprehensive procedure to measure levels and functional activity of complement regulator factor H (FH) encoded by CFH, the commonest genetic factor associated with aHUS and C3G, and present the results of the analysis of 28 uncharacterized, disease-associated, FH variants. Sixteen variants were not expressed in plasma and eight had significantly reduced functional activities that impact on complement regulation. In total, 24 of 28 CFH variants were unambiguously categorized as pathogenic and the nature of the pathogenicity fully documented for each. The data also reinforce the genotype-phenotype correlations that associate specific FH functional alterations with either aHUS or C3G and illustrate important drawbacks of the prediction algorithms dealing with variants located in FH functional regions. We also report that the novel aHUS-associated M823T variant is functionally impaired. This was unexpected and uncovered the important contribution of regions outside the N-terminal and C-terminal functional domains to FH regulatory activities on surfaces. Thus, our work significantly advances knowledge towards a complete functional understanding of the CFH genetic variability and highlights the importance of functional analysis of the disease-associated CFH variants. ; SRdeC is supported by the Spanish Ministerio de Economía y Competitividad/FEDER (grant number SAF2015-66287-R), the Seventh Framework Programme European Union Project EURenOmics (grantnumber 305608), and the Autonomous Region of Madrid (grantnumber S2010/BMD-2316). ; Peer reviewed
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C3 glomerulopathy-associated CFHR1 mutation alters FHR oligomerization and complement regulation
Tortajada, Agustín et alt. ; C3 glomerulopathies (C3G) are a group of severe renal diseases with distinct patterns of glomerular inflammation and C3 deposition caused by complement dysregulation. Here we report the identification of a familial C3G-associated genomic mutation in the gene complement factor H-related 1 (CFHR1), which encodes FHR1. The mutation resulted in the duplication of the N-terminal short consensus repeats (SCRs) that are conserved in FHR2 and FHR5. We determined that native FHR1, FHR2, and FHR5 circulate in plasma as homo- and hetero-oligomeric complexes, the formation of which is likely mediated by the conserved N-terminal domain. In mutant FHR1, duplication of the N-terminal domain resulted in the formation of unusually large multimeric FHR complexes that exhibited increased avidity for the FHR1 ligands C3b, iC3b, and C3dg and enhanced competition with complement factor H (FH) in surface plasmon resonance (SPR) studies and hemolytic assays. These data revealed that FHR1, FHR2, and FHR5 organize a combinatorial repertoire of oligomeric complexes and demonstrated that changes in FHR oligomerization influence the regulation of complement activation. In summary, our identification and characterization of a unique CFHR1 mutation provides insights into the biology of the FHRs and contributes to our understanding of the pathogenic mechanisms underlying C3G. Copyright © 2013, American Society for Clinical Investigation. ; Work in this report was funded by the Spanish Ministerio de Economía y Competitividad (SAF2011-26583), the Fundación Renal Iñigo Alvarez de Toledo and the 7FP European Union project EURenOmics to S. Rodríguez de Córdoba; the "Ramón Areces" Foundation, the Spanish Ministerio de Economía y Competitividad (SAF2011-22988), and the "Red Temática de Investigación Cooperativa en Cáncer(RTICC)" (RD06/0020/1001) to O. Llorca; the Spanish "Ministerio de Economía y Competitividad" (PI0900268) to P. Sánchez-Corral; the MRC UK (G0701298) to C.L. Harris; and the Spanish "Ministerio de Economía y Competitividad" (PS09/00122) to M. López Trascasa. In addition, this work was supported by a grant from the Autonomous Region of Madrid (S2010/BMD-2316) to S. Rodríguez de Córdoba, O. Llorca, M. López Trascasa, and P. Sánchez-Corral. M.C. Pickering is a Wellcome Trust Senior Fellow in Clinical Science (WT098476MA). H. Yébenes was supported by a postdoctoral contract by the RTICC, and C. Abarrategui-Garrido was supported by the "Fundación de Investigación Biomédica del Hospital Universitario La Paz." ; Peer reviewed
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