Suchergebnisse

Open Access.-- et al. ; Shelf life is an important quality trait for many fruit, including tomatoes. We report that enrichment of anthocyanin, a natural pigment, in tomatoes can significantly extend shelf life. Processes late in ripening are suppressed by anthocyanin accumulation, and susceptibility to Botrytis cinerea, one of the most important postharvest pathogens, is reduced in purple tomato fruit. We show that reduced susceptibility to B. cinerea is dependent specifically on the accumulation of anthocyanins, which alter the spreading of the ROS burst during infection. The increased antioxidant capacity of purple fruit likely slows the processes of overripening. Enhancing the levels of natural antioxidants in tomato provides a novel strategy for extending shelf life by genetic engineering or conventional breeding. © 2013 Elsevier Ltd. All rights reserved. ; E.B. and C.M. were supported by the European Union FP6 FLORA project (FOOD-CT-01730), and Y.Z., E.B., and C.M. are supported by the European Union FP7 ATHENA collaborative project (grant agreement 245121). Y.Z. is also supported by a Rotation Studentship from the John Innes Foundation. C.M. and E.B. were supported by the core strategic grant of the Biological and Biotechnological Science Research Council (BBSRC) to the John Innes Centre and are currently supported by the Institute Strategic Program Understanding and Exploiting Plant and Microbial Secondary Metabolism (BB/J004596/1) from the BBSRC. E.B. was supported by a short-term EMBO fellowship for undertaking part of the research reported in this paper. H.-J.S. was supported by grant BB/G042960/1 from the BBSRC and the John Innes Foundation, and A.G. and D.O. are supported by the Fundación Genoma (Calitom project) and the Spanish Ministry of Science and Education (project BIO2010-15384). This work benefited from the networking activities within the European-funded COST ACTION FA1106 QualityFruit. ; Peer Reviewed

We have studied a genomic library of introgression lines from the Solanum pimpinellifolium accession TO-937 into the genetic background of the "Moneymaker" cultivar in order to evaluate the accession's breeding potential. Overall, no deleterious phenotypes were observed, and the plants and fruits were phenotypically very similar to those of "Moneymaker," which confirms the feasibility of translating the current results into elite breeding programs. We identified chromosomal regions associated with traits that were both vegetative (plant vigor, trichome density) and fruit-related (morphology, organoleptic quality, color). A trichome-density locus was mapped on chromosome 10 that had not previously been associated with insect resistance, which indicates that the increment of trichomes by itself does not confer resistance. A large number of quantitative trait loci (QTLs) have been identified for fruit weight. Interestingly, fruit weight QTLs on chromosomes 1 and 10 showed a magnitude effect similar to that of QTLs previously defined as important in domestication and diversification. Low variability was observed for fruit-shape-related traits. We were, however, able to identify a QTL for shoulder height, although the effects were quite low, thus demonstrating the suitability of the current population for QTL detection. Regarding organoleptic traits, consistent QTLs were detected for soluble solid content (SSC). Interestingly, QTLs on chromosomes 2 and 9 increased SSC but did not affect fruit weight, making them quite promising for introduction in modern cultivars. Three ILs with introgressions on chromosomes 1, 2, and 10 increased the internal fruit color, making them candidates for increasing the color of modern cultivars. Comparing the QTL detection between this IL population and a recombinant inbred line population from the same cross, we found that QTL stability across generations depended on the trait, as it was very high for fruit weight but low for organoleptic traits. This difference in QTL stability may be due to a predominant additive gene action for QTLs involved in fruit weight, whereas epistatic and genetic background interactions are most likely important for the other traits. ; The authors want to thank the funding from grant AGL2015-65246-R (Spanish MINECO, co-financed by European Union FEDER programme), and the EU Framework Program Horizon 2020 COST Action FA1106 Quality Fruit for networking activities. WB was supported by a fellowship granted by the Universidad de Costa Rica and CSIC-Spain by way of a collaboration agreement between CSIC/UCR. GL-C was supported by a JAE-Doc contract by CSIC co-funded by the European Social Fund (ESF). ; Peer reviewed ; Peer Reviewed

This work was supported in part by the Spanish MINECO Project AGL2015-65246-R co-financed by EU FEDER, MINECO Project AGL2011-26957, and the Bilateral agreements of Scientific and Technological Cooperation between the Spanish National Research Council (CSIC) and the Italian National Research Council (CNR). Funding for this project was provided through TRADITOM. TRADITOM has received funding from the European Union's Horizon 2020 research and innovation programme under grant agreement No 634561. Networking activities were supported by COST action Fruit Quality FA 1106. ; Peer reviewed