Suchergebnisse

Not Available ; Bull spermatozoa contain different functional genes and many of them plays important roles in different stages of spermatogenesis, spermatozoa kinetics, fertilization as well as embryonic development. RNA deep sequencing is one of the preferred tools for absolute quantification of messenger RNA. The intention of the current study was to investigate the abundance of spermatozoal transcripts in categorized Frieswal (Holstein-Friesian X Sahiwal) crossbred bull semen through RNA deep sequencing. A total 1546561 and 1019308 numbers of reads were identified among good and poor quality bull spermatozoa based on their conception rate. Post mapping with Bos taurus reference genome identified 1,321,236 and 842,022 number of transcripts among good and poor quality RNA libraries, respectively. However, a total number of 3510 and 6759 functional transcripts were identified among good and poor quality bull spermatozoa, respectively. Most of the identified transcripts were related to spermatozoa functions, embryonic development and other functional aspects of fertilization. Wet laboratory validation of the top five selected transcripts (AKAP4, PRM1, ATP2B4, TRIM71 and SLC9B2) illustrated the significant (p < 0.01) level of expression in the good quality crossbred bull semen than the poor quality counterparts. The present study with comprehensive profiling of spermatozoal transcripts provides a useful non-invasive tool to understand the causes of as well as an effective way to predict male infertility in crossbred bulls. ; Science and Engineering Research Board, Government of India

Deltas are home to a large and growing proportion of the world's population, often living in conditions of extreme poverty. Deltaic ecosystems are ecologically significant as they support high biodiversity and a variety of fisheries, however these coastal environments are extremely vulnerable to climate change. The Ganges-Brahmaputra-Meghna (Bangladesh/India), the Mahanadi (India), and the Volta (Ghana) are among the most important and populous delta regions in the world and they are all considered at risk of food insecurity and climate change. The fisheries sector is vital for populations that live in the three deltas, as a source of animal protein (in Bangladesh and Ghana around 50–60% of animal protein is supplied by fish while in India this is about 12%) through subsistence fishing, as a source of employment and for the wider economy. The aquaculture sector shows a rapid growth in Bangladesh and India while in Ghana this is just starting to expand. The main exported species differ across countries with Ghana and India dominated by marine fish species, whereas Bangladesh exports shrimps and prawns. Fisheries play a more important part in the economy of Bangladesh and Ghana than for India, both men and women work in fisheries, with a higher proportion of women in the Volta then in the Asian deltas. Economic and integrated modelling using future scenarios suggest that changes in temperature and primary production could reduce fish productivity and fisheries income especially in the Volta and Bangladesh deltas, however these losses could be mitigated by reducing overfishing and improving management. The analysis provided in this paper highlights the importance of applying plans for fisheries management at regional level. Minimizing the impacts of climate change while increasing marine ecosystems resilience must be a priority for scientists and governments before these have dramatic impacts on millions of people's lives.