Suchergebnisse

The objective of this study is to establish the frequency of multi-drug resistant bacteria (MDR) isolated at the Avicenne Military Hospital as well as their levels of resistance to antibiotics. This is a retrospective study conducted over 7years (2015 to 2021), at the Avicenne Military Hospital. Were collected all bacteriological samples received for diagnostic purposes, from patients hospitalized for at least 48 hours. The identification of the bacterial strains was based on the study of their morphological, cultural, and biochemical characteristics on the BD Phoenix™ i100 Automated System, which also allows susceptibility testing. Detection of resistance phenotypes was completed by the conventional method of disc diffusion in an agar medium. The interpretation was based on CA-SFM/EUCAST guidelines. The overall frequency of MDR bacteria is 14,3% with a large predominance of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae (54,1%) followed by Acinetobacter baumannii resistant to ceftazidime and/or imipenem (28,1%), methicillin-resistant Staphylococcus aureus (9,3%), then Enterobacteriaceae resistant to carbapenems (5,2%), and finally Pseudomonas aeruginosa resistant to ceftazidime and/or imipenem which represented 3.3% of the MDR bacteria isolated. Co-resistance levels in MDR bacteria are high. The increasing frequency of multi-drug resistant bacteria highlights the importance of an appropriate antibiotic strategy and the necessity of the implementation of antibiotic resistance monitoring.

Objectives: Our study aims to assess the contribution of the Filmarray Meningitis/Encephalitis panel in the diagnosis and management of infectious meningitis by comparing it to conventional culture, while highlighting the epidemiology of the pathogens involved. Methods: This is a retrospective study that was carried out on 115 CSF samples from patients clinically suspected of meningitis and analyzed by conventional methods and by the Filmarray M / E panel test. This work was carried out over a period of 36 months from January 2018 toDecember2020 at the microbiology laboratory of the Avicenne Military Hospital in Marrakech. Results: 115 samples were documented. The filmarray M/E panel detected at least one pathogen in 26 samples, representing a positivity rate of 22.6%. The infectious agents implicated in meningitis were predominated by viruses (52%, n=14), followed by bacteria (44%, n=11); yeasts are clearly in the minority making 4% (n=1) of the positive samples. The cultures were positive in 6 cases. Streptococcus pneumoniae was the most involved microbial agent, constituting a rate of 50% (n=3) of the bacteria detected in the cultures. The concordance rate was 75% for the detection of Streptococcus pneumoniae. In this study, only one sample was positive on the filmarray Meningitis / Encephalitis panel and negative on culture. Conclusion: The Syndromic Meningitis / Encephalitis FilmArray-type multiplex PCR is a simple, rapid and reliable diagnostic tool allowing the implementation of an early and adapted therapeutic strategy. However, given the importance of susceptibility testing, the use of molecular tests should be carried out in addition to conventional microbiology.