Suchergebnisse

The spinothalamic tract (STT) neurons in the spinal dorsal horn play an important role in transmission and processing of nociceptive sensory information. Although transient receptor potential vanilloid type 1 (TRPV1) receptors are present in the spinal cord dorsal horn, their physiological function is not fully elucidated. In this study, we examined the role of TRPV1 in modulating neuronal activity of the STT neurons through excitatory and inhibitory synaptic inputs. Whole-cell patch-clamp recordings were performed on STT neurons labeled by a retrograde fluorescent tracer injected into the ventral posterior lateral (VPL) nucleus of the thalamus. Capsaicin (1 microM) increased the frequency of miniature excitatory postsynaptic currents (mEPSC) without changing the amplitude or decay time constant of mEPSC. In contrast, capsaicin had no distinct effect on GABAergic miniature inhibitory postsynaptic currents (mIPSC). Capsazepine (10 microM), a TRPV1 receptor antagonist, abolished the effect of capsaicin on mEPSCs. Capsazepine itself did not affect the baseline amplitude and frequency of mEPSC. The effect of capsaicin on mEPSC was also abolished by removal of external Ca(2+), but not by treatment with Cd(2+). Furthermore, capsaicin increased the firing activity of the STT neurons and this increase in neuronal activity by capsaicin was abolished in the presence of non-N-methyl-d-aspartic acid (NMDA) and NMDA receptor antagonists, 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline-2,3-dione (NBQX) and (R)-amino-5-phosphonovaleric acid (APV). These data suggest that activation of TRPV1 potentiates the glutamate release from excitatory terminals of primary afferent fibers and subsequently increases the neural activity of STT neurons of the rat spinal cord deep dorsal horn. ; This work was supported by a Korea Research Foundation Grant funded by the Korea Government (MOEHRD, Basic Research Promotion Fund, KRF-2005-015- E00034) and a grant of the Korea Healthcare Technology R&D Project, Ministry of Health, Welfare and Family Affairs (A080588).

Using the daily records derived from the synoptic weather stations and the NCEP/NCAR and ERA-Interim reanalysis data, the variability of the winter haze pollution (indicated by the mean visibility and number of hazy days) in the Beijing–Tianjin–Hebei (BTH) region during the period 1981 to 2015 and its relationship with the atmospheric circulations at middle–high latitude were analyzed in this study. The winter haze pollution in BTH had distinct inter-annual and inter-decadal variabilities without a significant long-term trend. According to the spatial distribution of correlation coefficients, six atmospheric circulation indices ( I 1 to I 6 ) were defined from the key areas in sea level pressure (SLP), zonal and meridional winds at 850 hPa (U850, V850), geopotential height field at 500 hPa (H500), zonal wind at 200 hPa (U200), and air temperature at 200 hPa (T200), respectively. All of the six indices have significant and stable correlations with the winter visibility and number of hazy days in BTH. In the raw (unfiltered) correlations, the correlation coefficients between the six indices and the winter visibility (number of hazy days) varied from 0.57 (0.47) to 0.76 (0.6) with an average of 0.65 (0.54); in the high-frequency ( < 10 years) correlations, the coefficients varied from 0.62 (0.58) to 0.8 (0.69) with an average of 0.69 (0.64). The six circulation indices together can explain 77.7 % (78.7 %) and 61.7 % (69.1 %) variances of the winter visibility and the number of hazy days in the year-to-year (inter-annual) variability, respectively. The increase in I c (a comprehensive index derived from the six individual circulation indices) can cause a shallowing of the East Asian trough at the middle troposphere and a weakening of the Siberian high-pressure field at sea level, and is then accompanied by a reduction (increase) of horizontal advection and vertical convection (relative humidity) in the lowest troposphere and a reduced boundary layer height in BTH and its neighboring areas, which are favorable for the formation of haze pollution in BTH winter, and vice versa. The high level of the prediction statistics and the reasonable mechanism suggested that the winter haze pollution in BTH can be forecasted or estimated credibly based on the optimized atmospheric circulation indices. Thus it is helpful for government decision-making departments to take action in advance in dealing with probably severe haze pollution in BTH indicated by the atmospheric circulation conditions.

Using the daily records derived from the synoptic weather stations and the NCEP/NCAR and ERA-Interim reanalysis data, the variability of the winter haze pollution (indicated by the mean visibility and number of hazy days) in the Beijing–Tianjin–Hebei (BTH) region during the period 1981 to 2015 and its relationship with the atmospheric circulations at middle–high latitude were analyzed in this study. The winter haze pollution in BTH had distinct inter-annual and inter-decadal variabilities without a significant long-term trend. According to the spatial distribution of correlation coefficients, six atmospheric circulation indices (I1 to I6) were defined from the key areas in sea level pressure (SLP), zonal and meridional winds at 850 hPa (U850, V850), geopotential height field at 500 hPa (H500), zonal wind at 200 hPa (U200), and air temperature at 200 hPa (T200), respectively. All of the six indices have significant and stable correlations with the winter visibility and number of hazy days in BTH. In the raw (unfiltered) correlations, the correlation coefficients between the six indices and the winter visibility (number of hazy days) varied from 0.57 (0.47) to 0.76 (0.6) with an average of 0.65 (0.54); in the high-frequency ( < 10 years) correlations, the coefficients varied from 0.62 (0.58) to 0.8 (0.69) with an average of 0.69 (0.64). The six circulation indices together can explain 77.7 % (78.7 %) and 61.7 % (69.1 %) variances of the winter visibility and the number of hazy days in the year-to-year (inter-annual) variability, respectively. The increase in Ic (a comprehensive index derived from the six individual circulation indices) can cause a shallowing of the East Asian trough at the middle troposphere and a weakening of the Siberian high-pressure field at sea level, and is then accompanied by a reduction (increase) of horizontal advection and vertical convection (relative humidity) in the lowest troposphere and a reduced boundary layer height in BTH and its neighboring areas, which are favorable for the formation of haze pollution in BTH winter, and vice versa. The high level of the prediction statistics and the reasonable mechanism suggested that the winter haze pollution in BTH can be forecasted or estimated credibly based on the optimized atmospheric circulation indices. Thus it is helpful for government decision-making departments to take action in advance in dealing with probably severe haze pollution in BTH indicated by the atmospheric circulation conditions.