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Carro, Lorena/0000-0001-8833-642X; Trujillo, Martha E./0000-0002-9193-1673; Jarrin, Raul Riesco/0000-0001-8257-7601 ; WOS: 000425219900040 ; PubMed: 29148367 ; Two actinobacterial isolates, strains SG15(T) and SGB14(T), were recovered through a microbial diversity study of nitrogen fixing nodules from Pisum sativum plants collected in Salamanca (Spain). The taxonomic status of these isolates was determined using a polyphasic approach and both presented chemotaxonomic and morphological properties consistent with their classification in the genus Micromonospora. For strains SG15(T) and SGB14(T), the highest 16S rRNA gene sequence similarities were observed with Micromonospora coxensis JCM 13248(T) (99.2 %) and Micromonospora purpureochromogenes DSM 43821(T) (99.4 %), respectively. However, strains SG15(T) and SGB14(T) were readily distinguished from their phylogenetic neighbours both genetically and phenotypically indicating that they represent two new Micromonospora species. The following names are proposed for these species: Micromonospora phytophila sp. nov. type strain SG15(T) (=CECT 9369(T); =DSM 105363(T)), and Micromonospora luteiviridis sp. nov. type strain SGB14(T) (=CECT 9370(T); =DSM 105362(T)). ; Ministerio de Economia y CompetitividadSpanish Government [GL2014-52735-P]; School of Biology (Newcastle University) ; This work was supported by the project GL2014-52735-P to MET from the Ministerio de Economia y Competitividad and a research post-doctoral contract to LC from the School of Biology (Newcastle University).

The data from this study are available in the manuscript and additional file. Genomic data are deposited in public databases (accession numbers are provided in Additional File 1: Table S1). The genomic sequence data of Trichococcus strain ES5 that supports the findings of this study have been deposited in GenBank with accession codes GCA_900067165.1, GCF_900067165.1. Supplementary information accompanies this paper at https://doi.org/10. 1186/s12864-019-6410-x. ; The genus Trichococcus currently contains nine species: T. flocculiformis, T. pasteurii, T. palustris, T. collinsii, T. patagoniensis, T. ilyis, T. paludicola, T. alkaliphilus, and T. shcherbakoviae. In general, Trichococcus species can degrade a wide range of carbohydrates. However, only T. pasteurii and a non-characterized strain of Trichococcus, strain ES5, have the capacity of converting glycerol to mainly 1,3-propanediol. Comparative genomic analysis of Trichococcus species provides the opportunity to further explore the physiological potential and uncover novel properties of this genus. ; This research was supported by the European Research Council under the European Union's Seventh Framework Programme / ERC Grant Agreement (323009) and by the Gravitation grant (024.002.002) of the Netherlands Ministry of Education, Culture and Science. The work conducted by the U.S. Department of Energy Joint Genome Institute (DOE-JGI), a DOE Office of Science User Facility, was supported by the Office of Science of the DOE under Contract No. DE-AC02-05CH11231. The funding bodies played no role in the design of the study and collection, analysis, and interpretation of data and in writing the manuscript. ...

Abstract The genus Marinobacterium belongs to the family Alteromonadaceae within the class Gammaproteobacteria and was reported in 1997. Currently the genus Marinobacterium contains 16 species. Marinobacterium rhizophilum CL-YJ9T was isolated from sediment associated with the roots of a plant growing in a tidal flat of Youngjong Island, Korea. The genome of the strain CL-YJ9T was sequenced through the Genomic Encyclopedia of Type Strains, Phase I: KMG project. Here we report the main features of the draft genome of the strain. The 5,364,574 bp long draft genome consists of 58 scaffolds with 4762 protein-coding and 91 RNA genes. Based on the genomic analyses, the strain seems to adapt to osmotic changes by intracellular production as well as extracellular uptake of compatible solutes, such as ectoine and betaine. In addition, the strain has a number of genes to defense against oxygen stresses such as reactive oxygen species and hypoxia. ; This work was performed under the auspices of the US Department of Energy's Office of Science, Biological and Environmental Research Program, and by the University of California, Lawrence Berkeley National Laboratory under contract No. DE-AC02-05CH11231. This work was also supported in part by a research program (PE99513) of the Korea Institute of Ocean Science and Technology (KIOST) and by an East Asian Seas Time series-1 (EAST-1) funded by the Ministry of Oceans and Fisheries, Korea, and the BK21+ project of the Korean Government