Suchergebnisse

Adenoviruses are a common cause of upper respiratory tract infections in children and the source of acute respiratory disease outbreaks among military recruits. Named after the human adenoid tissue it was first isolated in, the adenoviridae are non-enveloped, double-stranded DNA viruses with over 50 serotypes1. Yet to date vaccines have been developed for only two serotypes, and no adenovirus antiviral drugs have been approved by the FDA, highlighting the need for more research. In virus research, detection the presence of a particular virus is of especial interest when developing animal or cell models. As human pathogens often do not infect common lab animals or certain cell types, researchers must alter either the host or the virus in order to produce a working model. This may involve genetically engineering host cells to express a need receptor protein, or passaging cells multiple times in the presence of the virus, anticipating that the virus will evolve pathogenicity. Thus, the ability to detect if a virus has replicated in a host is key to determining the viability of a cell or animal model. Here we attempt to develop an ELISA detection assay for adenovirus d1327. ELISA assay is especially usefully for virus detection because of its specificity and easily identifiable positive result. Antibodies specific to the virus of interest bind the virus to the plate, while enzyme conjugates provide a visible signal if the virus is present.