Suchergebnisse

In this study, we manufactured various ratios of polycaprolactone (PCL)/gelatin (GE) highly aligned electrospun nanofibrous scaffolds (ENs) to investigate the effects of polymer ratio on tenogenic differentiation activity. For biological assessments, the cell proliferation rate was optimal in the PCL/GE (9:1) group. Interestingly, however, the tenogenic differentiation rate was best for the PCL/GE (7:3) group. From our outcomes, we established that a poly-blending mix of PCL/GE (7:3) is a promising ratio for tenogenic differentiation. Thus, our findings may provide for an effective mesh to promote tenogenic differentiation of ENs in future tendon tissue engineering applications. ; This work was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) & funded by the Korean government (MSIP&MOHW) (No. ...

Titanium implants (Ti) have been widely used in several medical fields. In clinical practice, Ti can become contaminated with bacteria through a variety of mechanisms. This contamination can lead to implant failure and serious infections. In this study, we aimed to develop a new, hybrid Ti with good biocompatibility and antibacterial properties by immobilizing ceftazidime (CFT) onto the Ti surface through polydopamine (PDA) and polyethyleneimine (PEI) chemistry. Hybrid Ti was confirmed by assessing the cell proliferation of human adipose-derived stem cells using a cell counting. The biofilm formation across the Ti surface of two bacterial strains associated with nosocomial infections, Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus, was evaluated by scanning electron microscopy. The viability of the bacteria exposed to Ti surface was evaluated by cell counting. Our results clearly demonstrate that the bacterial biofilm formation as well as bacterial viability was significantly reduced on the hybrid Ti as compared to the control, Ti alone. Collectively, the Ti surface was successfully modified to form the hybrid Ti exhibiting good biocompatibility and antibacterial properties through PDA, PEI, and CFT grafting. Within the limitations of this in vitro study, we conclude that the hybrid Ti may be useful for successful implant treatment. ; This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Korean government (MSIT) (No. ...

Many drugs have progressed through preclinical and clinical trials and have been available - for years in some cases -before being recalled by the FDA for unanticipated toxicity in humans. One reason for such poor translation from drug candidate to successful use is a lack of model systems that accurately recapitulate normal tissue function of human organs and their response to drug compounds. Moreover, tissues in the body do not exist in isolation, but reside in a highly integrated and dynamically interactive environment, in which actions in one tissue can affect other downstream tissues. Few engineered model systems, including the growing variety of organoid and organ-on-a-chip platforms, have so far reflected the interactive nature of the human body. To address this challenge, we have developed an assortment of bioengineered tissue organoids and tissue constructs that are integrated in a closed circulatory perfusion system, facilitating inter-organ responses. We describe a three-tissue organ-on-a-chip system, comprised of liver, heart, and lung, and highlight examples of inter-organ responses to drug administration. We observe drug responses that depend on inter-tissue interaction, illustrating the value of multiple tissue integration for in vitro study of both the efficacy of and side effects associated with candidate drugs. ; Defense Threat Reduction Agency (DTRA) under Space and Naval Warfare Systems Center Pacific (SSC PACIFIC) [N6601-13-C-2027]; Comprehensive Cancer Center of Wake Forest University NCI CCSG [P30CA012197] ; The authors gratefully thank Dr. Pedro Baptista for aid in the drug metabolism studies, Steven Forsythe and Meiei Wan for technical aid in maintaining liver and cardiac organoid viability, and Dipasri Konar and Katherine Crowell for technical aid in the lung-on-a-chip operation. The authors gratefully acknowledge funding by the Defense Threat Reduction Agency (DTRA) under Space and Naval Warfare Systems Center Pacific (SSC PACIFIC) Contract No. N6601-13-C-2027. The publication of this material does not constitute approval by the government of the findings or conclusions herein. Proteomics and Metabolomics Core Lab services are supported by the Comprehensive Cancer Center of Wake Forest University NCI CCSG P30CA012197 grant.