Suchergebnisse

Making the distinction between drought and water scarcity is not trivial, because they often occur simultaneously. In this study, we used Copulas to quantify the relationship between drought and water scarcity. Beijing&ndash ; Tianjin&ndash ; Hebei Metropolitan Areas (BTHMA) was chosen as the study area. Standard Precipitation and Evapotranspiration Index (SPEI) and water exploitation index plus (WEI+) was chosen to represent metrological drought and water scarcity. Inverse Distance Weighted method was used for spatial analysis of SPEI and WEI+, and Archimedean Copula was used to establish two-dimensional joint probability distribution of SPEI and WEI+. The results are as follows: (1) The southern part of the study area was wetter. The middle part was drier, with moderate drought happened for most times. (2) WEI+ of Beijing and Tianjin showed significant decreasing trends from 2000 to 2015, while WEI+ of Hebei Province did not, which indicated that Hebei Province is facing much severer water scarcity situation than Beijing and Tianjin. (3) Gumbel copula was the best-fitting model to establish the joint probability distribution of SPEI and WEI+. The condition probability provided a probability distribution of water scarcity under different drought conditions, which can provide technical support for government managers during policy making.

In the present work, Listeria monocytogenes was used as the target strain to investigate the sterilising potential and mechanism of high power pulse microwave (HPPM). Results showed that the inactivation was positively correlated with the pulse frequencies and operating times. The count of Listeria monocytogenes was decreased by 5.09 log CFU/mL under 200 Hz for 9 min, which was used as the optimised condition to further explore the sterilisation mechanism. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images showed that the L. monocytogenes cells of untreated group presented intact surfaces, clear boundary, and its intracellular contents distributed uniformly in the cytoplasm. Following treatment, the cell wall surfaces began to deform in small areas, and cell membranes were severely ruptured, thus resulting in the appearance of electron transmission areas. Extracellular protein and nucleic acid contents, represented by OD260 nm and OD280 nm, increased with the increase in operating time significantly. After treatment, SDS-PAGE profiles of whole-cell proteins displayed that the protein bands became lighter or even disappeared. Na+ K+-ATPase activities and intracellular ATP content decreased by 72.97 and 79.09%, respectively. This was consistent with the cell viability of L. monocytogenes observed by confocal laser scanning microscopy. Overall, the sterilisation mechanism of HPPM on L. monocytogenes may be caused by membrane damage, intracellular component leakage, and energy metabolism hindrance.