Suchergebnisse

The objective of this study was to evaluate the effect of inclusion of different levels of protected lysine and methionine on the nutritional parameters and protein metabolites of lambs. The experiment was carried out at the Capim Branco Experimental Farm, at Uberlândia Federal University, during the months of September and October 2016. Five blood lambs ½ Dorper × ½ Santa Inês, approximately eight months old and with an average weight of 50 kg, were distributed in a Latin square scheme 5 × 5 (five treatments and five replicates). The treatments consisted of the inclusion in the diet of different levels of lysine and methionine protected from ruminal degradation (MicroPEARLS LM®): 0 g, 8 g, 16 g, 24 g, and 32 g. The diet was composed of corn silage and concentrate in a ratio of 30V:70C. The experiment was divided into five phases. Each phase had a duration of fifteen days (ten days of adaptation of the animals to the experimental diets and five days of data collection). The animals were kept in individual metabolic cages. A digestibility assay was performed to determine the apparent dry matter intake and digestibility (DMI/DDMI), nitrogen balance, and protein metabolites. The DMI (kg day-1) and in relation to the metabolic weight presented a positive linear equation, and was highest for the treatment with 32 g of amino acids, as well as the ingested nitrogen and nitrogen balance, being positive in all treatments. There was no difference (P > 0.05) in the DDMI. The crude protein and urea concentrations remained above the recommended levels and the concentration of creatinine remained below the recommended level. The inclusion of protected amino acids up to 32 g increases DMI and urinary nitrogen excretion without negatively affecting digestibility. The protein metabolism was altered, without causing damage to the performance of the animals.

Voluntary consumption (VolC) and ingestive behaviour (IB) of lambs fed diets containing different percentages (0.0; 7.5; 15.0 and 22.5%) of babassu mesocarp bran (I-BMB) were evaluated. The trial was at the Federal University of Tocantins, Campus de Araguaína-TO. Twenty lambs were evaluated using a completely randomized design, and five replications were performed. VolC data was obtained by measuring total volumes of both food supplied and food remaining after feeding. IB was evaluated throughout two, non-consecutive 24-h periods, with observations were recorded every five minutes that described the following behavioural variables: resting (REST), rumination (RUM), intake (INT) and chewing (CHEW). The particle size was also measured using a Penn State particle separator. All VolC parameters (dry matter intake, DMI; crude protein intake, CPI; neutral detergent fibre intake, NDFI; and acid detergent fibre intake, ADFI) increased linearly with I-BMB intake. I-BMB consumption did not interfere with water intake, nor did it affect I-BMB did not affect the duration of DMI, NDFI and ADFI. However, linear increases of rumination and chewing activities associated with DMI, NDFI and ADFI were observed as I-BMB levels increased. In summary, effects of inclusion I-BMB in diets of lambs included increased voluntary consumption, and reduced rumination and chewing, but not ingestion, efficiency. When economically favorable, these data suggest that inclusion of up to 22.5% I-BMB within balanced diets should be applied.

Objetivou-se estimar os intervalos de referência dos parâmetros bioquímicos de ovelhas lactantes nos trópicos. Foram compiladas informações de até 290 ovelhas lactantes no período de 2006 a 2017. Todos os animais eram saudáveis e não passaram por condições de desnutrição forçada. Os animais foram criados em diferentes sistemas de manejo (pasto, confinamento total, semiconfinamento, confinamento coletivo e/ou individual). O perfil bioquímico constituiu informações de colesterol, triglicerídeos, frutosamina, HDL (lipoproteína de alta densidade), VLDL (lipoproteína de muito baixa densidade), proteínas totais, ácido úrico, ureia, albumina, creatinina, cálcio, fósforo e magnésio, AST (aspartato aminotransferase), GGT (gama glutamil transferase) e fosfatase alcalina (FA). Os valores de referência foram estimados através do teste de Dixon, quando paramétrico e por boostrap quando não paramétrico, sendo o intervalo de confiança a 95% de probabilidade. Os intervalos estimados foram 36,3 - 94; 262,22 - 450,66; 7 - 43; 18,6 - 69,4; 1,4 - 8,4; 0,61 - 1,66; 5,4 - 11; 0 - 1,4; 8,4 - 61,5; 1,9 - 3,57 para colesterol, frutosanima, trigligeríceos, HDL, VLDL, creatinina, PT, ácido úrico, ureia e albumina, respectivamente. Para as enzimas e o perfil mineral foram 59 - 160; 37,9 - 127,8; 30 - 190; 8,23 - 12,45; 3,7 - 8,5; 1,7 - 3,8 para AST, GGT, FA, Ca, P, e Mg, respectivamente. Conclui-se que os parâmetros bioquímicos das ovelhas lactantes nos trópicos são discrepantes aos determinados na literatura.

The objective of this study was to evaluate the use of extrused roughage on nutritional and metabolic parameters in sheep. Eighteen 3-year-old sheep in metabolic cages were randomly assigned to two treatments: corn silage and extruded roughage. Dry matter intake (DMI) and water intake were calculated as the difference between offered feed and orts left. Ruminal movement was assessed through auscultation of the rumen for five minutes, glycemia and other metabolites through blood collection by jugular venipuncture, and feeding behavior by observation every 5 minutes for 24 hours. DMI per animal, both as a percentage of live weight and in relation to metabolic weight, was higher for animals fed extrused roughage. The extrused roughage treatment also resulted in reduced ruminal movement and higher dry matter digestibility (DMD), glycemia and drinking water intake. Animals fed with extrused roughage reduced the time of rumination and total chewing, thus increasing ingestion, chewing and rumination efficiency. Blood concentrations of glucose and urea were higher in animals fed extrused roughage, while cholesterol concentration was lower. We conclude that the use of extrused roughage promotes improvement in the nutritional parameters of sheep by increasing DMI and DMD, and reducing chewing and rumination times, without causing disorders in energy and protein metabolism.

Industrialization of the Babassu coconut for edible oil production generates many co-products and has great potential to be used in ruminant nutrition. The aim of this study was to evaluate the effect of different inclusions of endocarp meal type II of Babassu coconut (BEMII) on intake and digestibility of sheep diets. Twenty non-castrated male sheep with an undefined breed were distributed in a completely randomized design with four treatments and five replications. The animals received an experimental diet with increasing inclusions of BEMII (0.0%, 7.5%, 15.0%, and 22.5%). Increasing additions of the coproduct negatively influenced dry matter intake (g/BW and g/kg0.75). Crude protein intake (in grams) per day decreased linearly with the inclusion of BEMII, presenting a reduction of 49.35 g/d. The inclusion of 7.5% showed the best response for energy balance among all inclusions and presented the closest nitrogen balance value when compared to the control group; therefore, this inclusion percentage is the best for endocarp meal utilization.

Lysine and methionine are the most limiting amino acids for ruminants; their inclusion in the free form is not effective, since they are quickly degraded by ruminal microorganisms, making the fulfillment of the requirements of metabolizable protein difficult. The objective of this study was to evaluate the effects of including different levels of lysine and methionine, protected from ruminal degradation, in the diet of lambs; more specifically, their effects on intake, digestibility, nitrogen balance and ingestive behavior. The experiment was conducted at the Experimental Farm Capim Branco, Federal University of Uberlândia, from September to October 2016. Five 1/2Dorper x 1/2Santa Inês lambs, at approximately eight months of age and an average weight of 50 kg, were distributed in a 5x5 Latin square design, with five treatments and five replicates. The treatments consisted of different levels (0 g, 8 g, 16 g, 24 g and 32 g) of lysine and methionine added to the diet, protected from ruminal degradation (MicroPEARLS LM ®). The feed was composed of corn silage and concentrate (30V: 70C), offered twice daily at 08h00 and 16h00. The experiment lasted 75 days and was divided into five phases. Each phase lasted 15 days, with 10 days for adaptation of the animals and five days of data collection. The animals were kept in individual metabolic cages, with a feeder, drinker and salt shaker. A digestibility assay was performed to determine the consumption and apparent digestibility coefficients of dry matter (DM), crude protein (CP), neutral detergent fiber and acid (NDF/NDA). Urine volume and density, stool weight and fecal score, nitrogen balance and ingestive behavior were recorded. In relation to metabolic and body weight, DM intake (kg day-1) presented a positive linear response with the inclusion of amino acids, as well as the CP intake, NDF intake, ingested nitrogen and nitrogen balance, which were positive in all treatments. There was no difference (P < 0.05) in the digestibility coefficients of DM, CP and NDF. Water consumption was high in all treatments, but did not differ by treatment (P > 0.05). Ingestive behavior was not influenced (P > 0.05). The inclusion of up to 32 g of protected lysine and methionine increases DM and nutrient intake, without negatively affecting digestibility. The high CP intake induced an increase in the urinary nitrogen excretion, and consequently, an increase in the energy expenditure.

The aim of this study was to evaluate the influence of crude glycerin (CG) suplementation in sheep diets during pregnancy and transition period. Twenty-four Lacaune x East Friesian genotypes sheep were randomly assigned to four treatments. Diet was composed of roughage: tifton hay, and standard concentrate for the categories, being these similar among the treatments, whereas the CG differed for the supplements in (zero, 1,5, 3,0 and 4,5% of dry matter) being It added to the animals' drinking water. The sheep received supplementation during all pregnancy and lactation. Dry matter intake during gestation was reduced with the higher supplementation of CG in the animals' drinking water, presenting a linear decreasing response and remaining until delivery. Throughout lactation, the variable presented a linear behavior with the supplementation of CG on the eighteenth day of this phase, after a quadratic behavior was observed until the end of the evaluated period. The beta-hydroxybutyrate and non-esterified fatty acids levels were not influenced by CG supplementation throughout the gestation period. CG suplementation conveyed in water did not affect negatively milk production and composition.

The objective of this study was to evaluate the effects of crude glycerin (CG) supplementation of dairy ewes during pregnancy and early lactation on the body weight and measurements of offspring from birth to weaning. Twenty-four lambs from 24 Lacaune x East Friesian genotype ewes were evaluated. The sheep were distributed in four treatments that differed in crude glycerin supplementation of 0%, 1.5%, 3.0%, and 4.5%, as well as the concomitant adjustment of dry matter and water intake. Body measurements of lambs were recorded every two weeks from birth until weaning. Body weight was recorded daily. Multiple regression analysis revealed that supplementation with CG explained 5.2% of the variation in the weight of lambs at birth (LW), accompanied by the body condition score of the ewe (12.3%), sex of lamb (19.2%), and live weight of the ewes (26.5%); r2 was 63%. According to the predictions of the model adjusted for simulated scenarios, the leanest lambs were derived from ewes weighing between 40 and 50 kg, with body condition scores from 4 to 5, and that did not receive CG at intermediate levels. Heavier lambs, with LW above 5.5 kg, were derived from ewes that weighed between 80 to 90 kg with low body condition scores of 3, and that received 1.5% CG. Supplementation with CG between 2% and 4% improves the weight of the lambs at birth. Body measurements of lambs were not influenced by dietary supplementation of ewes with CG.

The objective of this study was to evaluate the effect of inclusion of different levels of protected lysine and methionine on the nutritional parameters and protein metabolites of lambs. The experiment was carried out at the Capim Branco Experimental Farm, at Uberlândia Federal University, during the months of September and October 2016. Five blood lambs ½ Dorper × ½ Santa Inês, approximately eight months old and with an average weight of 50 kg, were distributed in a Latin square scheme 5 × 5 (five treatments and five replicates). The treatments consisted of the inclusion in the diet of different levels of lysine and methionine protected from ruminal degradation (MicroPEARLS LM®): 0 g, 8 g, 16 g, 24 g, and 32 g. The diet was composed of corn silage and concentrate in a ratio of 30V:70C.The experiment was divided into five phases. Each phase had a duration of fifteen days (ten days of adaptation of the animals to the experimental diets and five days of data collection). The animals were kept in individual metabolic cages. A digestibility assay was performed to determine the apparent dry matter intake and digestibility (DMI/DDMI), nitrogen balance, and protein metabolites. The DMI (kg day-1) and in relation to the metabolic weight presented a positive linear equation, and was highest for the treatment with 32 g of amino acids, as well as the ingested nitrogen and nitrogen balance, being positive in all treatments. There was no difference (P > 0.05) in the DDMI. The crude protein and urea concentrations remained above the recommended levels and the concentration of creatinine remained below the recommended level. The inclusion of protected amino acids up to 32 g increases DMI and urinary nitrogen excretion without negatively affecting digestibility. The protein metabolism was altered, without causing damage to the performance of the animals.

The study aimed to determine the biochemical reference ranges for lambs from birth to 1 year of age in the tropics. Data were obtained from experiments performed in several institutions and commercial farms using Santa Inês, Dorper, Lacaune, Morada Nova, Bergamacia and Suffolk lambs reared under different conditions (grazing, feedlot, semi-feedlot) from 2006 to 2017. Serum energy-, protein-, mineral-, and enzyme-related metabolites were evaluated. The metabolic energy profile included data from glucose, cholesterol, triglycerides, fructosamine, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol, and very-low-density lipoprotein cholesterol; the metabolic protein profile included total protein, uric acid, urea, albumin, and creatinine; the metabolic mineral profile included data on calcium, phosphorus, and magnesium; and the metabolic enzymatic profile included the enzymes aspartate aminotransferase, gamma-glutamyl transferase, alkaline phosphatase, and creatine kinase. The reference ranges were estimated using confidence intervals with a 95% confidence level, and percentiles were estimated or bootstrapped nonparametrically when the data were not normally distributed. The serum biochemical reference ranges determined for lambs are strongly divergent from those established by one of the most cited books on the topic, especially considering the high serum urea and cholesterol concentrations and low levels of blood glucose observed. Therefore, the serum biochemical reference ranges for lambs from birth to 1 year of age in the tropics differ from international data, which consider adult sheep in temperate climate zones.