Aufsatz(elektronisch)#121. Dezember 2023
Cryo-EM structure of bovine chaperonin TRiC/CCT in open conformation
In: Vestnik Moskovskogo universiteta. Seria 16. Biologia, Band 78, Heft №3s, 2023, S. 40-46
In this work, conditions were selected for obtaining a sample of eukaryotic chaperonin TRiC
suitable for studying by cryo-electron microscopy. Using the method of differential scanning
(time-resolved) fluorimetry, the temperature stability of protein samples at different concentrations of salt and glycerol was compared, and then the selected conditions were used to prepare the
sample for microscopy. As a result, the structure of bovine TRiC in an open conformation was
obtained at 4.42 Å resolution.