Suchergebnisse

[EN] A naphthalimide-based two-photon probe (AHGa) for the detection of cell senescence is designed. The probe contains a naphthalimide core, an L-histidine methyl ester linker, and an acetylated galactose bonded to one of the aromatic nitrogen atoms of the L-histidine through a hydrolyzable N-glycosidic bond. Probe AHGa is transformed into AH in senescent cells resulting in an enhanced fluorescent emission intensity. In vivo detection of senescence is validated in mice bearing tumor xenografts treated with senescence-inducing chemotherapy. ; The authors thank the financial support from the Spanish Government (Projects MAT2015-64139-C4-1-R) and the Generalitat Valenciana (Project PROMETEOII/2014/047). B.L-T. is grateful to the Spanish Ministry of Economy for her PhD grant. I.G. thanks the Generalitat Valenciana for her PhD grant. A.B. thanks the Spanish Government for the financial support "Juan de la Cierva-Incorporacion". M.R. is grateful to "La Caixa" foundation for his PhD scholarship. Work in the M.S. group was funded by the CNIO and by grants from Spanish Ministry of Economy, the European Regional Development Fund (SAF project) the European Research Council (ERC Advanced Grant), and the Botin Foundation and Banco Santander (Santander Universities Global Division). D.M.-E. was funded by a "Ramon y Cajal" Programme Senior Grant and the "RETOS" National Programme for Research (MINECO), Cancer Research UK, and the CRUK Cambridge Centre Early Detection Programme. ; Lozano-Torres, B.; Galiana, I.; Rovira, M.; Garrido-García, EM.; Chaib, S.; Bernardos Bau, A.; Muñoz-Espín, D. (2017). An OFF-ON Two-Photon Fluorescent Probe for Tracking Cell Senescence in Vivo. Journal of the American Chemical Society. 139(26):8808-8811. https://doi.org/10.1021/jacs.7b04985 ; S ; 8808 ; 8811 ; 139 ; 26

Cellular senescence contributes to organismal development, aging, and diverse pathologies, yet available assays to detect senescent cells remain unsatisfactory. Here, we designed and synthesized a lipophilic, biotin-linked Sudan Black B (SBB) analogue suitable for sensitive and specific, antibody-enhanced detection of lipofuscin-containing senescent cells in any biological material. This new hybrid histo-/immunochemical method is easy to perform, reliable, and universally applicable to assess senescence in biomedicine, from cancer research to gerontology. ; Acknowledgments The authors would like to acknowledge Dr Ioannis Dotsikas help in performing the HPLC analysis and thank Dr Vassilis Myrianthopoulos for helpful suggestions. The authors' work is funded by the European Union's Seventh Framework Programme (project INsPiRE), the Greek GSRT program of Excellence II (Aristeia II), the Danish Cancer Society, the Danish National Research Foundation (DNRF125). ; Sí