Suchergebnisse

This work was supported by the Spanish Ministerio de Innovación y Ciencia (AGL2016-75536-P), the Agencia Estatal de Investigación (AEI) and the European regional fund (ERF), the Generalitat de Catalunya (2017SGR170) and the Centre de Referència en Biotecnologia (XeRBa). CERCA Programme from the Generalitat de Catalunya is also acknowledged. M.B.-J. has a grant from COLCIENCIAS (Republic of Colombia). P.B.-P. has a grant from the Spanish Ministry of Economy, Industry and Competitiveness (BES-2017-081296). L.R.-R. is supported by the Beatriu de Pinos postdoctoral programme of the Government of Catalonia's Secretariat for Universities and Research of the Ministry of Economy and Knowledge. ; Altres ajuts: MICITT/AGL2016-75536-P ; Bacteriophages can package part of their host's genetic material, including antibiotic resistance genes (ARGs), contributing to a rapid dissemination of resistances among bacteria. Phage particles containing ARGs were evaluated in meat, pork, beef and chicken minced meat, and ham and mortadella, purchased in local retailer. Ten ARGs (bla, bla, bla, bla, bla, qnrA, qnrS, mecA, armA and sul1) were analyzed by qPCR in the phage DNA fraction. The genes were quantified, before and after propagation experiments in Escherichia coli, to evaluate the ability of ARG-carrying phage particles to infect and propagate in a bacterial host. According to microbiological parameters, all samples were acceptable for consumption. ARGs were detected in most of the samples after particle propagation indicating that at least part of the isolated phage particles were infectious, being sul1the most abundant ARG in all the matrices followed by β-lactamase genes. ARGs were also found in the phage DNA fraction of thirty-seven archive chicken cecal samples, confirming chicken fecal microbiota as an important ARG reservoir and the plausible origin of the particles found in meat. Phages are vehicles for gene transmission in meat that should not be underestimated as a risk factor in the global crisis of antibiotic ...

© The Author(s) 2021. ; Aquatic environments are key niches for the emergence, evolution and dissemination of antimicrobial resistance. However, the population diversity and the genetic elements that drive the dynamics of resistant bacteria in different aquatic environments are still largely unknown. The aim of this study was to understand the population genomics and evolutionary events of Escherichia coli resistant to clinically important antibiotics including aminoglycosides, in anthropogenic and natural water ecosystems. Here we show that less different E. coli sequence types (STs) are identified in wastewater than in rivers, albeit more resistant to antibiotics, and with significantly more plasmids/cell (6.36 vs 3.72). However, the genomic diversity within E. coli STs in both aquatic environments is similar. Wastewater environments favor the selection of conserved chromosomal structures associated with diverse flexible plasmids, unraveling promiscuous interplasmidic resistance genes flux. On the contrary, the key driver for river E. coli adaptation is a mutable chromosome along with few plasmid types shared between diverse STs harboring a limited resistance gene content. ; Work carried out in the Institute of Biomedicine and Biotechnology (IBBTEC) was funded by the Ministry of Economy and Competitiveness (grant BFU2017-86378-P). The work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO BES-2015-073164) and the European Union's Horizon 2020 Research and Innovation Programme (grant 773830, OH-EJP-H2020-JRP-AMR-2-WORLDCOM).

Aquatic environments are key niches for the emergence, evolution and dissemination of antimicrobial resistance. However, the population diversity and the genetic elements that drive the dynamics of resistant bacteria in different aquatic environments are still largely unknown. The aim of this study was to understand the population genomics and evolutionary events of Escherichia coli resistant to clinically important antibiotics including aminoglycosides, in anthropogenic and natural water ecosystems. Here we show that less different E. coli sequence types (STs) are identified in wastewater than in rivers, albeit more resistant to antibiotics, and with significantly more plasmids/cell (6.36 vs 3.72). However, the genomic diversity within E. coli STs in both aquatic environments is similar. Wastewater environments favor the selection of conserved chromosomal structures associated with diverse flexible plasmids, unraveling promiscuous interplasmidic resistance genes flux. On the contrary, the key driver for river E. coli adaptation is a mutable chromosome along with few plasmid types shared between diverse STs harboring a limited resistance gene content. ; Acknowledgements: The authors acknowledge Isabel Cuesta and Sara Monzon from the Bioinformatics Unit at the Institute of Health Carlos III for their contributions and advice to the study. The authors thank Almudena Casamayor from the Microbial Identification and Characterization Unit at the VISAVET Health Surveillance Centre for her support in the bacterial identification by MALDI-TOF mass spectrometry. The authors acknowledge Iciar Rodriguez-Avial from the Microbiology Unit at the San Carlos Hospital for providing the plazomicin to perform MIC evaluations. Work carried out in the Institute of Biomedicine and Biotechnology (IBBTEC) was funded by the Ministry of Economy and Competitiveness (grant BFU2017-86378-P). The work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO BES-2015-073164) and the European Union's Horizon 2020 Research and Innovation Programme (grant 773830, OH-EJP-H2020-JRP-AMR-2-WORLDCOM).