Suchergebnisse

There are few instances in the literature on urban school reform that have closely studied specific school buildings to describe the grant resource appropriation process. This study attempts to fill that gap by developing case studies of two middle schools with divergent profiles in resource utilization through micro-ethnographies. One school exhibited optimal engagement with opportunities made available through a large federally funded grant, whereas the second school failed to do the same through this initiative. We investigated how the unique features of these schools and key individuals within them intersected with the structure of the grant program to shape the nature of school engagement. These two case study narratives illuminate the range of factors that might be implicated in the implementation of school reform initiatives, particularly in urban settings.

This paper is published by the Energy Research Centre, University of Cape Town under the Creative Commons Attribution-ShareAlike 4.0 International Licence (CC BY-SA). Full details of this licence are available at: http://creativecommons.org/licenses/by-sa/4.0/ ; Agro-industries have the potential to make a substantial contribution to sustainable energy supply in Africa, including energy access in rural areas. This paper focuses on the drivers and barriers to wider use of cogeneration from sugarcane bagasse in Malawi as there is a potential for the technology to enable access to electricity in rural areas. The paper gives an overview of the policy landscape for the energy sector and the sugar industry in Malawi. The research involved site visits, focus group discussions, and individual semi-structured interviews with participants from key government departments, businesses, research institutes and international agencies. It was found that energy sector reform, the proposed feed-in tariff for renewable energy, and risk are the key issues for investment in this area.

Agro-industries have the potential to make a substantial contribution to sustainable energy supply in Africa, including energy access in rural areas. This paper focuses on the drivers and barriers to wider use of cogeneration from sugarcane bagasse in Malawi as there is a potential for the technology to enable access to electricity in rural areas. The paper gives an overview of the policy landscape for the energy sector and the sugar industry in Malawi. The research involved site visits, focus group discussions, and individual semi-structured interviews with participants from key government departments, businesses, research institutes and international agencies. It was found that energy sector reform, the proposed feed-in tariff for renewable energy, and risk are the key issues for investment in this area.Keywords: biomass, renewable energy, electricity

Early adaptive responses to hypoxia are essential for cell survival, but their nature and underlying mechanisms are poorly known. We have studied the post-transcriptional changes in the proteome of mammalian cells elicited by acute hypoxia and found that phosphorylation of eukaryotic elongation factor 2 (eEF2), a ribosomal translocase whose phosphorylation inhibits protein synthesis, is under the precise and reversible control of O(2) tension. Upon exposure to hypoxia, phosphorylation of eEF2 at Thr(56) occurred rapidly (<15 min) and resulted in modest translational arrest, a fundamental homeostatic response to hypoxia that spares ATP and thus facilitates cell survival. Acute inhibitory eEF2 phosphorylation occurred without ATP depletion or AMP kinase activation. Furthermore, eEF2 phosphorylation was mimicked by prolyl hydroxylase (PHD) inhibition with dimethyloxalylglycine or by selective PHD2 siRNA silencing but was independent of hypoxia-inducible factor α stabilization. Moreover, overexpression of PHD2 blocked hypoxic accumulation of phosphorylated eEF2. Therefore, our findings suggest that eEF2 phosphorylation status (and, as a consequence, translation rate) is controlled by PHD2 activity. They unravel a novel pathway for cell adaptation to hypoxia that could have pathophysiologic relevance in tissue ischemia and cancer. ; Spanish Ministry of Science and Health ; Andalusian Government

24 páginas, 8 figuras, 2 tablas. ; Early adaptive responses to hypoxia are essential for cell survival, but their nature and underlying mechanisms are poorly known. We have studied the post-transcriptional changes in the proteome of mammalian cells elicited by acute hypoxia and found that phosphorylation of eukaryotic elongation factor 2 (eEF2), a ribosomal translocase whose phosphorylation inhibits protein synthesis, is under the precise and reversible control of O2 tension. Upon exposure to hypoxia, phosphorylation of eEF2 at Thr56 occurred rapidly (<15 min) and resulted in modest translational arrest, a fundamental homeostatic response to hypoxia that spares ATP and thus facilitates cell survival. Acute inhibitory eEF2 phosphorylation occurred without ATP depletion or AMP kinase activation. Furthermore, eEF2 phosphorylation was mimicked by prolyl hydroxylase (PHD) inhibition with dimethyloxalylglycine or by selective PHD2 siRNA silencing but was independent of hypoxia-inducible factor α stabilization. Moreover, overexpression of PHD2 blocked hypoxic accumulation of phosphorylated eEF2. Therefore, our findings suggest that eEF2 phosphorylation status (and, as a consequence, translation rate) is controlled by PHD2 activity. They unravel a novel pathway for cell adaptation to hypoxia that could have pathophysiologic relevance in tissue ischemia and cancer. ; Research was supported by the Spanish Ministry of Science and Health, the Marcelino Botin Foundation, and the Andalusian Government. ; Peer reviewed