Suchergebnisse

OBJECTIVES: Masenchymal stem cells (MSCs) have pluripotent differentiation properties that confirmed to differentiate into myocyte, adipocyte, osteoblast, neuron and chondrocyte when specific culture conditions and stimuli are applied. In bone, both adipocytes and osteoblasts are derived from bone marrow MSCs (BMSCs) and production of these cells has been reported as reciprocal processes. The bone mass disequilibrium causes osteoporosis, as a result of elevated adipogenic differentiation accompanied by reducing bone formation. Therefore, in this study the effect of Ligustrum japonicum fructus (Waxleaf privet) on the adipogenesis and osteoblast differentiation was investigated in BMSCs. METHODS: The fruits of L. japonicum were extracted with dichloromethane and methanol, and the combined extracts were concentrated. Differentiation of BMSCs was performed by changing the medium into adipocyte and osteoblast differentiation supplied by Promocell GmbH. The cellular lipid was stained with Oil Red O and the alkaline phosphatase (ALP) activity was measured using a colormetric assay kit (Biovision, Inc.). The relative protein levels were measured by immunoblotting assay. RESULTS: Presense of L. japonicum fructus extract (LJE) inhibited the cellular lipid accumulation in a dose-dependent manner. Consistent with the effects on lipid accumulation, the adipocyte specific genes including PPARγ, C/EBPα and SREBP1c was down regulated by treatment with LJE. Moreover, treatment with LJE enhanced osteoblast differentiation observed as increased ALP activity and upregulated the proteALP, BMP-2 and osteocalcine protein levels. CONCLUSIONS: The results indicated that LJE may prevent bone loss by inhibiting adipogenesis while activating the osteogenic differentiation in BMSCs. Therefore, LJE possess the potential to be utilized as a source of nutraceutical agents against osteoporosis. FUNDING SOURCES: This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP).

3C 84 (NGC 1275) is the radio source at the centre of the Perseus cluster and exhibits a bright radio jet. We observed the source with the Global Millimeter VLBI Array (GMVA) between 2008 and 2015, with a typical angular resolution of ∼50 μas. The observations revealed a consistent double nuclear structure separated by ∼770 gravitational radii assuming a black hole mass of 3.2 × 108 M⊙. The region is likely too broad and bright to be the true jet base anchored in the accretion disc or black hole ergosphere. A cone and parabola were fit to the stacked (time averaged) image of the nuclear region. The data did not strongly prefer either fit, but combined with a jet/counter-jet ratio analysis, an upper limit on the viewing angle to the inner jet region of ≤35° was found. This provides evidence for a variation of the viewing angle along the jet (and therefore a bent jet) within ∼0.5 pc of the jet launching region. In the case of a conical jet, the apex is located ∼2400 gravitational radii upstream of the bright nuclear region and up to ∼600 gravitational radii upstream in the parabolic case. We found a possible correlation between the brightness temperature and relative position angle of the double nuclear components, which may indicate rotation within the jet. © 2021 The Author(s). ; This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2021R1A6A3A01086420). JAH was supported by Korea Research Fellowship Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT, South Korea (2018H1D3A1A02032824) and the research grant (2021R1C1C1009973). S-SL was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MIST) (2020R1A2C2009003). J-YK is supported for this research by the International Max-Planck Research School (IMPRS) for Astronomy and Astrophysics at the University of Bonn and Cologne. This research was supported by an appointment to the NASA Postdoctoral Program at the Goddard Space Flight Center, administered by Universities Space Research Association through a contract with NASA. RL acknowledges the support of the Spanish Ministerio de Economía y Competitividad (grant PID2019-108995GB-C21), the Consejería de Economía, Conocimiento, Empresas y Universidad, Junta de Andalucía (grant P18-FR-1769), and the Consejo Superior de Investigaciones Científicas (grant 2019AEP112). GFP is supported for this research by the International Max-Planck Research School (IMPRS) for Astronomy and Astrophysics at the University of Bonn and Cologne. ST and MK acknowledge support via NRF grant 2019R1F1A1059721. This research has made use of data obtained with the Global Millimeter VLBI Array (GMVA), which consists of telescopes operated by the MPIfR, IRAM, Onsala, Metsahovi, Yebes, the Korean VLBI Network, the Green Bank Observatory, and the Very Long Baseline Array (VLBA). The VLBA is an instrument of the NRAO, which is a facility of the National Science Foundation operated under cooperative agreement by Associated Universities, Inc. The data were correlated at the correlator of the Max Planck Institute for Radioastronomy (MPIfR) in Bonn, Germany. This work made use of the Swinburne University of Technology software correlator, developed as part of the Australian Major National Research Facilities Programme and operated under licence. ; With funding from the Spanish government through the Severo Ochoa Centre of Excellence accreditation SEV-2017-0709. ; Peer reviewed