Analyses of Virulence and Genetic Diversity Among Xanthomonas Axonopodis Pv . Vignicola Isolates from Different Cowpea Varieties
In: HELIYON-D-22-29223
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In: HELIYON-D-22-29223
SSRN
In: Scientific African, Band 19, S. e01548
ISSN: 2468-2276
In: ABUAD International Journal of Natural and Applied Sciences, Band 1, Heft 1, S. 30-38
Extracts and isolated compounds of Allium sativum (garlic) have been found to be of health benefit. The study was aimed at assessing the effects of crude garlic extracts on urinopathogens of pregnant women, as well as to compare the antibacterial and genetic profiles of Nigerian indigenous and exotic varieties of garlic. Biodata and urine samples were collected from two hundred (200) healthy pregnant women attending antenatal clinics. The urine samples were subjected to urinalysis and bacteriological investigations. The subjects were 20 - 43 (31.03 ± 1.46) years old, with modal age 25-30 years. Urinalysis of subjects' urine samples showed no nitrituria, haematuria and bilirubinuria. However, glucosuria (1.5%), ketonuria (3%), leukocyturia (15%) and proteinuria (24%) were detected. Bacterial loads of the urine samples range from 0 to 1100 (with mean value of 315.72) cfu/mL, an indication of non-urinary tract infection bacteriuria. Bacteria isolated from the urine samples were: Escherichia coli, Klebsiella pneumoniae, Klebsiella variicola, Enterobacter cloacae, Pseudomonas aeruginosa, Proteus mirabilis, Citrobacter freundii, Corynebacterium accolens, Actinomyces urogenitalis, Luteococcus sanguinis and Bacillus cereus among others. The bacterial isolates showed high prevalence of multidrug resistant bacteria, with resistance to 2-8 drugs. The filtrates of crushed and centrifuged bulbs of both the indigenous and exotic varieties of garlic produced high antibacterial activities, while both ethanolic and methanolic extracts of garlic did not produce antibacterial activity. The indigenous variety showed higher antibacterial activities and protein qualities than the exotic variety, with both varieties showing genetic diversity. In conclusion, the Nigerian indigenous garlic was found to be of high antibacterial and protein qualities; and for maximal health benefit garlic needs to be chewed or crushed and consumed directly.
In: ABUAD International Journal of Natural and Applied Sciences, Band 2, Heft 1, S. 35-41
Garcinia kola otherwise known as bitter kola has been used in traditional African medicine for centuries, and is considered to have many beneficial properties. The study was aimed at assessing the nutritional and bioactive components of bitter kola chips. Freshly harvested seeds of Garcinia kola were purchased from local markets in Ado Ekiti, Nigeria. The seeds were thoroughly washed with clean water, de-husked, sliced and fast-dried at room temperature under high air current, to obtain bitter kola chips. The dried bitter kola chips were ground into fine powder using an electric blender. The fine powder was then analysed for its proximate, minerals, vitamins, phytochemicals and essential oil content. The results showed low moisture content (8.47 %), moderate levels of protein (12.7 %), fat (6.27 %), ash (7.47 %) and crude fibre (6.27 %), as well as very high levels of carbohydrate (58.77 %) and metabolizable energy (3000 Kcal/Kg). The micronutrients present in bitter kola consists of Fe2+, Zn2+, Mg2+, Ca2+, K+, PO3-, ascorbic acid, thiamine, niacin, and riboflavin at concentrations of 9.37, 0.23, 23.33, 238.33, 26.67, 323.33, 18.23, 0.27, 0.13 and 0.60 mg/100g respectively. The bitter kola chips contained high levels of alkaloids (628.33±10.41 mg/100g), saponins (161.67±7.64 mg/100g), and tannins (233.33±7.64 mg/100g); moderate levels of flavonoids (58.33±5.41 mg/100g), phytates (63.33±2.89), oxalates (90.00±5.00) and oxygen radical absorption capacity (13.23±0.25); while the anti-protease activity and cyanogenic glycosides levels were very low. GCMS analysis of the oil components of G. kola chips showed the presence of molecules such as Trichloroacetic acid, Ar-tumerone, Tumerone, which are of significant importance to human health. Sensory evaluation showed high preference for the bitter kola chips than the fresh seeds.