Suchergebnisse

In this study, the total phenolic content, the antioxidant and antiproliferative activities of onion waste extracts were characterized. Some phenolic compounds present in the extracts were also identified and quantified by HPLC-DAD. Additionally, an in-silico analysis was performed to identify the phenolic compounds with the highest intestinal absorption and Caco-2 permeability. The onion extract possessed a high amount of phenolic compounds (177 ± 9 mg/g extract) and had an effective antioxidant capacity measured by ABTS, FRAP and DPPH assays. Regarding the antiproliferative activity, the onion extracts produced cell cycle arrest in the S phase with p53 activation, intrinsic apoptosis (mitochondrial membrane potential modification) and caspase 3 activation. Likewise, onion waste increased intracellular ROS with possible NF-kB activation causing a proteasome down regulation. In addition, the extracts protected the intestine against oxidative stress induced by H2O2. According to the in-silico analysis, these results could be related to the higher Caco-2 permeability to protocatechuic acid. Therefore, this study provides new insights regarding the potential use of these types of extract as functional ingredients with antioxidant and antiproliferative properties and as medicinal agents in diseases related to oxidative stress, such as cancer. In addition, its valorization would contribute to the circular economy. ; :This work was supported by the Grants: CIBEROBN, CB06/03/1012, 2008, Ministerio de Ciencia e Innovación-Fondo Europeo de Desarrollo Regional (PID2019-104915RB-I00), Fondo Social Europeo-Gobierno de Aragón (B16_20R) and SUDOE (Redvalue, SOE1/PI/E0123). This research was alsofunded by EUROPEAN UNION'S H2020 research and innovation program under Marie Sklodowska-Curie grant agreement number 801586. G.M. gratefully acknowledges the support from the Miguel Servet Program (MS19/00092; Instituto de Salud Carlos III). M.P. acknowledges the support from the Gobierno de Aragón fellowship (CUS/581/2020).

Olive pomace (OP) is the main residue that results from olive oil production. OP is richin bioactive compounds, including polyphenols, so its use in the treatments of diseases related tooxidative stress, such as cancer, could be considered. The present work aimed to study the biologicalproperties of different OP extracts, obtained by ohmic heating-assisted extraction and conventionalheating, using water and 50% ethanol, in the treatment and prevention of colorectal cancer throughCaco-2 cell models. Additionally, an in-silico analysis was performed to identify the phenolic intestinalabsorption and Caco-2 permeability. The extracts were chemically characterized, and it was found thatthe Ohmic-hydroethanolic (OH-EtOH) extract had the highest antiproliferative effect, probably dueto its higher content of phenolic compounds. The OH-EtOH induced potential modifications in themitochondrial membrane and led to apoptosis by cell cycle arrest in the G1/S phases with activationof p53 and caspase 3 proteins. In addition, this extract protected the intestine against oxidative stress(ROS) caused by H2O2. Therefore, the bioactive compounds present in OP and recovered by applying agreen technology such as ohmic-heating, show promising potential to be used in food, nutraceutical,and biomedical applications, reducing this waste and facilitating the circular economy. ; This work was supported by the Grants from MCIN, CIBEROBN (CB06/03/1012), RedMultimetdrugs (RED2018-102471-T), and the Government of Aragón (B16-20R), the Interreg SudoeProgram (REDVALUE, SOE1/P1/E0123) through the European Regional Development Fund (FEDER) and the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategicfunding of UIDB/04469/2020 unit.

Olive pomace (OP) is the main residue that results from olive oil production. OP is rich in bioactive compounds, including polyphenols, so its use in the treatments of diseases related to oxidative stress, such as cancer, could be considered. The present work aimed to study the biological properties of different OP extracts, obtained by ohmic heating-assisted extraction and conventional heating, using water and 50% ethanol, in the treatment and prevention of colorectal cancer through Caco-2 cell models. Additionally, an in-silico analysis was performed to identify the phenolic intestinal absorption and Caco-2 permeability. The extracts were chemically characterized, and it was found that the Ohmic-hydroethanolic (OH-EtOH) extract had the highest antiproliferative effect, probably due to its higher content of phenolic compounds. The OH-EtOH induced potential modifications in the mitochondrial membrane and led to apoptosis by cell cycle arrest in the G1/S phases with activation of p53 and caspase 3 proteins. In addition, this extract protected the intestine against oxidative stress (ROS) caused by H2O2. Therefore, the bioactive compounds present in OP and recovered by applying a green technology such as ohmic-heating, show promising potential to be used in food, nutraceutical, and biomedical applications, reducing this waste and facilitating the circular economy. ; This work was supported by the Grants from MCIN, CIBEROBN (CB06/03/1012), Red Multimetdrugs (RED2018-102471-T), and the Government of Aragón (B16-20R), the Interreg Sudoe Program (REDVALUE, SOE1/P1/E0123) through the European Regional Development Fund (FEDER) and the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit. ; info:eu-repo/semantics/publishedVersion

Ion channels have recently attracted attention as potential mediators of skin disease. Here, we explored the consequences of genetically encoded induction of the cell volume-regulating Ca2+-activated KCa3.1 channel (Kcnn4) for murine epidermal homeostasis. Doxycycline-treated mice harboring the KCa3.1+-transgene under the control of the reverse tetracycline-sensitive transactivator (rtTA) showed 800-fold channel overexpression above basal levels in the skin and solid KCa3.1-currents in keratinocytes. This overexpression resulted in epidermal spongiosis, progressive epidermal hyperplasia and hyperkeratosis, itch and ulcers. The condition was accompanied by production of the pro-proliferative and pro-inflammatory cytokines, IL-β1 (60-fold), IL-6 (33-fold), and TNFα (26-fold) in the skin. Treatment of mice with the KCa3.1-selective blocker, Senicapoc, significantly suppressed spongiosis and hyperplasia, as well as induction of IL-β1 (-88%) and IL-6 (-90%). In conclusion, KCa3.1-induction in the epidermis caused expression of pro-proliferative cytokines leading to spongiosis, hyperplasia and hyperkeratosis. This skin condition resembles pathological features of eczematous dermatitis and identifies KCa3.1 as a regulator of epidermal homeostasis and spongiosis, and as a potential therapeutic target ; This work was supported by the Government of Aragón (DGA-METIC–consortium; B04_17R), FP7-PEOPLE-MC-CIG to RK, FIS-CB06/07/1036 to PG and RK, ERC-2014-StG-638284, DPI2016-75458-R, T39_17R to EP; FIS-PI16/02112 to ALGO; NHLBI-R01-HL080173, NCRRP20-RR018751 to HM. JLG received a DGA-PhD scholarship (C072/2014). BMB was supported by the NCATS (UL1 TR001860 and linked award TL1 TR001861)