Suchergebnisse

Adenoviruses are a common cause of upper respiratory tract infections in children and the source of acute respiratory disease outbreaks among military recruits. Named after the human adenoid tissue it was first isolated in, the adenoviridae are non-enveloped, double-stranded DNA viruses with over 50 serotypes1. Yet to date vaccines have been developed for only two serotypes, and no adenovirus antiviral drugs have been approved by the FDA, highlighting the need for more research. In virus research, detection the presence of a particular virus is of especial interest when developing animal or cell models. As human pathogens often do not infect common lab animals or certain cell types, researchers must alter either the host or the virus in order to produce a working model. This may involve genetically engineering host cells to express a need receptor protein, or passaging cells multiple times in the presence of the virus, anticipating that the virus will evolve pathogenicity. Thus, the ability to detect if a virus has replicated in a host is key to determining the viability of a cell or animal model. Here we attempt to develop an ELISA detection assay for adenovirus d1327. ELISA assay is especially usefully for virus detection because of its specificity and easily identifiable positive result. Antibodies specific to the virus of interest bind the virus to the plate, while enzyme conjugates provide a visible signal if the virus is present.

Explanatory style refers to our habitual ways of explaining bad events. According to the reformulation of the learned helplessness model, stability and globality of explanatory style influence the extent of helplessness following bad events. However, most research involving explanatory
style looks not at helpless behavior per se but rather at more distant consequences (like depression) ostensibly involving helplessness. In the present research, we explicitly investigated helplessness and its relationship to explanatory style. Study One found that students (n = 40)
who explained bad events with stable and global causes were less likely than their more optimistic counterparts to take active steps to improve their course performance following a poor grade. In contrast, internality of explanatory style was positively correlated with active coping attempts.
Study Two found that young adults (n = 72) who explained bad events with stable and global causes were less likely to take active steps to feel better when they experienced symptoms of illness. Internality of explanatory style was not significantly correlated with attempts to feel better.