Suchergebnisse

BARD-US Israel Binational Agricultural Research and Development Fund ; WI Experiment Station as Hatch Project from Sao Paulo, Brazil ; Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) ; Colombian government ; BARD-US Israel Binational Agricultural Research and Development Fund: IS-4799-15 ; WI Experiment Station as Hatch Project from Sao Paulo, Brazil: WIS01240 ; Colombian government: 497/2009 ; In ruminants, uterine pulses of prostaglandin (PG) F-2 alpha characterize luteolysis, while increased PGE(2)/PGE(1) distinguish early pregnancy. This study evaluated intrauterine (IU) infusions of PGF(2 alpha) and PGE(1) pulses on corpus luteum (CL) function and gene expression. Cows on day 10 of estrous cycle received 4 IU infusions (every 6 h; n = 5/treatment) of saline, PGE(1) (2 mg PGE(1)), PGF(2 alpha) (0.25 mg PGF(2 alpha)), or PGE(1) + PGF(2 alpha). A luteal biopsy was collected at 30 min after third infusion for determination of gene expression by RNA-Seq. As expected, IU pulses of PGF(2 alpha) decreased (P 0.10) compared to saline cows. Increased circulating concentrations of the metabolite of PGF(2 alpha) (PGFM; after PGF(2 alpha) and PGE(1) + PGF(2 alpha)) and the metabolite PGE (PGEM; after PGE(1) and PGE(1) + PGF(2 alpha)) demonstrated that PGF(2 alpha) and PGE(1) are entering bloodstream after IU infusions. Thus, IU pulses of PGF(2 alpha) and PGE(1) allow determination of changes in luteal gene expression that could be relevant to understanding luteolysis and pregnancy. Unexpectedly, by third pulse of PGE(1), there is complete blockade of either PGF(2 alpha) transport to the CL or PGF(2 alpha) action by PGE(1) resulting in complete inhibition of transcriptomic changes following IU PGF(2 alpha) pulses. Summary Sentence Treatment with PGF(2 alpha) induced dramatic changes in expression of 955 genes, based on RNA-Seq after the third PGF(2 alpha) pulse (FDR < 0.01), whereas simultaneous treatment with pulses of PGE(1) blocked luteolysis and gene expression induced by PGF(2 alpha).