In the frame of a project funded by the European Food Safety Authority (Prima Phacie), effort was put into identifying and testing qualitative plant-pest risk assessment schemes for their suitability in supporting risk management decisions for the European Union. Five schemes were tested, two largely based on the EPPO scheme and three adapted from schemes used in non-European countries. We report the results from the application of these schemes as applied to Xanthomonas citri strains causing Citrus Bacterial Canker, in regard to the risk of its entry, establishment and spread, as well as its potential impact. For this pathogen, three entry pathways into the EU risk assessment area were considered: a) import of fresh citrus fruits, b) import of ornamental rutaceous plants or plant parts, and c) illegal entry of plant propagative material. With the current EU measures in place, of the five schemes tested, two indicated path (c) as that of the highest risk, whereas the other three suggested path (a) as such. This discrepancy is due to the different level of details the components of the risk of entry are considered in each scheme. Most schemes suggested that the establishment potential lay around the mid-range of possible values. All schemes indicated a high rate for potential spread (primarily through human activities) and a medium to high rate for impact potential. The effectiveness of risk management measures was evaluated by comparing results of assessments with and without management measures in place. (Texte integral)
Asiatic canker, caused by Xanthomonas citri pv. citri, is a major threat to worldwide citriculture. Three pathotypes differing in host range and hypersensitive reactions toward citrus species have been defined. Whereas pathotypes Aw and A* have a restricted host range, X. citri pv. citri pathotype A infects a broader range including most commercial citrus species and hybrids and can cause important economic losses in tropical and subtropical areas. Pathotype A strains, especially those assigned to lineage 1, were implicated in the major geographical expansion of X. citri pv. citri during the 20th century from their native area, Asia (Pruvost et al. 2014). X. citri pv. citri is listed as a quarantine pathogen in the European Union (EU) – Directive 2000/29/EC annex II A1. Martinique (France) is an outermost region of the EU in the eastern Caribbean Sea. Canker lesions were first observed at Morne Rouge, Martinique in June 2014 on grapefruit (Citrus paradisi), mandarin (C. reticulata), Tahiti lime (C. latifolia), and Valencia and Washington Navel oranges (C. sinensis). Official diagnostics, including bacterial isolations on YPGA or KC semiselective medium (Pruvost et al. 2005), PCR-based identification with 4/7 primers (Hartung et al. 1993), and pathogenicity tests, were performed following the EPPO standard PM7/44 (www.eppo.int) and identified isolates as X. citri pv. citri. Three strains isolated in Martinique in 2014 from grapefruit or Tahiti lime were further characterized (LL074-4, LL077-2, and LL079). Multilocus sequence analysis (MLSA) targeting six housekeeping genes (atpD, dnaK, efp, gltA, gyrB, and lepA) (Almeida et al. 2010; Bui Thi Ngoc et al. 2010) identified Martinique strains as X. citri pv. citri with 100% sequence identity to the type strain LMG 9322. Using MLVA-31 targeting 31 minisatellites, Martinique strains were assigned to lineage 1 composed of pathotype A strains (Pruvost et al. 2014). All strains were inoculated by a detached leaf assay onto Mexican lime SRA 140 (C. aurantifolia), sweet orange Washington Navel SRA 102, and grapefruit Henderson SRA 336 (Bui Thi Ngoc et al. 2010). All inoculated leaves produced typical erumpent, callus-like tissue at wound sites. Xanthomonas-like colonies were reisolated from lesions that had developed. Boiled suspensions were assayed by PCR with 4/7 primers and produced the expected amplicon, fulfilling Koch's postulates. No lesions developed on the negative control consisting of sterile 0.01M tris buffer pH 7.2. This is the first report of X. citri pv. citri in Martinique and to our knowledge in the Caribbean region. Surprisingly, all strains collected to date in Martinique grew on YPGA supplemented with 300 mg liter−1 copper sulfate even when no extensive copper spray programs have been used, suggesting that copper-resistant strains may have been introduced. Disease is contained by tree removal and burning and the situation is presently under apparent control although positive trees were sporadically detected in 2015 in backyards or small orchards at Le Lorrain and Saint Pierre. An extensive surveillance program is currently implemented in Martinique for quarantine pathogens of citrus. (Texte intégral)
Following a request from the European Commission, the European Food Safety Authority (EFSA) Plant Health (PLH) Panel analysed a dossier submitted by the Japanese authorities in order to clarify the host status of Citrus junos with regard to Xanthomonas citri pv. citri and Xanthomonas citri pv. aurantifolii, causal agents of citrus bacterial canker, and to indicate whether C. junos fruit could represent a pathway for the introduction of citrus bacterial canker into the European Union. In a previous opinion in the year 2014, the EFSA PLH Panel concluded that commercial fresh citrus fruit is generally pathway and that no commercially important Citrus species or variety can be considered as immune to citrus bacterial canker. In the current assessment, the EFSA PLH Panel analysed the two scientific papers provided by the Japanese authorities, as well as 16 additional papers identified through a systematic literature review. The PLH Panel considered that the conclusions of its previous opinion remain valid and that convergent lines of evidence provide sufficient demonstration that C. junos is a host of X. citri pv. citri and X. citri pv. aurantifolii. Therefore, there is no reason to consider the C. junos fruit differently from other citrus species. Consequently, the assessment of the general citrus fruit pathway from the 2014 opinion still applies. Uncertainties on these conclusions are a result of the scarce scientific evidence published on this subject in addition to the methodological and reporting limitations of the published papers.
Following a request from the European Commission, the European Food Safety Authority (EFSA) Plant Health (PLH) Panel analysed a dossier submitted by the Japanese authorities in order to clarify the host status of Citrus junos with regard to Xanthomonas citri pv. citri and Xanthomonas citri pv. aurantifolii, causal agents of citrus bacterial canker, and to indicate whether C. junos fruit could represent a pathway for the introduction of citrus bacterial canker into the European Union. In a previous opinion in the year 2014, the EFSA PLH Panel concluded that commercial fresh citrus fruit is generally pathway and that no commercially important Citrus species or variety can be considered as immune to citrus bacterial canker. In the current assessment, the EFSA PLH Panel analysed the two scientific papers provided by the Japanese authorities, as well as 16 additional papers identified through a systematic literature review. The PLH Panel considered that the conclusions of its previous opinion remain valid and that convergent lines of evidence provide sufficient demonstration that C. junos is a host of X. citri pv. citri and X. citri pv. aurantifolii. Therefore, there is no reason to consider the C. junos fruit differently from other citrus species. Consequently, the assessment of the general citrus fruit pathway from the 2014 opinion still applies. Uncertainties on these conclusions are a result of the scarce scientific evidence published on this subject in addition to the methodological and reporting limitations of the published papers.
Following a request from the European Commission, the European Food Safety Authority (EFSA) Plant Health (PLH) Panel analysed a dossier submitted by the Japanese authorities in order to clarify the host status of Citrus junos with regard to Xanthomonas citri pv. citri and Xanthomonas citri pv. aurantifolii, causal agents of citrus bacterial canker, and to indicate whether C. junos fruit could represent a pathway for the introduction of citrus bacterial canker into the European Union. In a previous opinion in the year 2014, the EFSA PLH Panel concluded that commercial fresh citrus fruit is generally pathway and that no commercially important Citrus species or variety can be considered as immune to citrus bacterial canker. In the current assessment, the EFSA PLH Panel analysed the two scientific papers provided by the Japanese authorities, as well as 16 additional papers identified through a systematic literature review. The PLH Panel considered that the conclusions of its previous opinion remain valid and that convergent lines of evidence provide sufficient demonstration that C. junos is a host of X. citri pv. citri and X. citri pv. aurantifolii. Therefore, there is no reason to consider the C. junos fruit differently from other citrus species. Consequently, the assessment of the general citrus fruit pathway from the 2014 opinion still applies. Uncertainties on these conclusions are a result of the scarce scientific evidence published on this subject in addition to the methodological and reporting limitations of the published papers.