Aufsatz(elektronisch)#17. November 2019
Chromatographic purification of the enzyme preparation from the cultural liquid of Pleurotus ostreatus
In: Proceedings of the National Academy of Sciences of Belarus, Biological Series, Band 64, Heft 4, S. 467-471
ISSN: 2524-230X
The primary purification of the proteolytic enzyme preparation from the Pleurotus ostreatus culture fluid was carried out. Methods of salting out, dialysis, ion-exchange chromatography on DEAEand KM-sepharose were used. It was shown that during the chromatography on KM-sepharose, the enzyme preparation from the culture liquid was divided into three fractions, one of which possessed milk-clotting activity (МСА). Chromatography on DEAE-sepharose allowed us to reach the significant purity of the enzyme with МСА.