Suchergebnisse

Funding Information: This work received funding support from Funda??o para a Ci?ncia e a Tecnologia (FCT), Portugal through the project PTDC/AGR-FOR/4218/2012, and the research units UID/Multi/04551/2013 (GREEN-IT), UIDB/00239/2020 (CEF) and UIDP/04035/2020 (GeoBioTec). C.A. acknowledges the FCT Investigator Program (IF/00376/2012/CP0165/CT0003). T.F.J. acknowledges FCT (PD/BD/113475/2015) and ITQB NOVA International PhD Program Plants for Life (PD/00035/2013). A.R.F. and S.A. are supported by the European Union?s Horizon 2020 research and innovation programme, project PlantaSYST (SGA-CSA No. 739582 under FPA No. 664620). Funding Information: Funding: This work received funding support from Fundação para a Ciência e a Tecnologia (FCT), Portugal through the project PTDC/AGR-FOR/4218/2012, and the research units UID/Multi/04551/2013 (GREEN-IT), UIDB/00239/2020 (CEF) and UIDP/04035/2020 (GeoBioTec). C.A. acknowledges the FCT Investigator Program (IF/00376/2012/CP0165/CT0003). T.F.J. acknowledges FCT (PD/BD/113475/2015) and ITQB NOVA International PhD Program Plants for Life (PD/00035/2013). A.R.F. and S.A. are supported by the European Union's Horizon 2020 research and innovation programme, project PlantaSYST (SGA-CSA No. 739582 under FPA No. 664620). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. ; Actinorhizal plants have been regarded as promising species in the current climate change context due to their high tolerance to a multitude of abiotic stresses. While combined salt-heat stress effects have been studied in crop species, their impact on the model actinorhizal plant, Casuarina glauca, has not yet been fully addressed. The effect of single salt (400 mM NaCl) and heat (control at 26/22 °C, supra optimal temperatures at 35/22 °C and 45/22 °C day/night) conditions on C. glauca branchlets was characterised at the physiological level, and stress-induced metabolite changes were characterised by mass spectrometry-based metabolomics. C. glauca could withstand single salt and heat conditions. However, the harshest stress condition (400 mM NaCl, 45 °C) revealed photosynthetic impairments due to mesophyll and membrane permeability limitations as well as major stress-specific differential responses in C and N metabolism. The increased activity of enzymatic ROS scavengers was, however, revealed to be sufficient to control the plant oxidative status. Although C. glauca could tolerate single salt and heat stresses, their negative interaction enhanced the effects of salt stress. Results demonstrated that C. glauca responses to combined salt-heat stress could be explained as a sum of the responses from each single applied stress. ; publishersversion ; published