Suchergebnisse

JR-C is supported by European Union FEDER funds, Fondo de Investigación Sanitaria (PI16/00113 ; ISCIII, Spain).

European Union FEDER funds (EU); Fondo de Investigacion Sanitaria (FIS), Instituto de Salud Carlos III [PI16/00113]; Plan de Ciencia, Tecnología e Innovacion de Asturias [IDI-2018-000152]; Sara Borrell program Instituto de Salud Carlos III [CD19/00120]

This work was supported by European Union FEDER funds and the Fondo de Investigación Sanitaria (FIS, PI08/0570 and PI12/0053). J.R.-C. is a recipient of a FPU grant from the Ministerio de Educación. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

European Union FEDER funds; Fondo de Investigacion Sanitaria [FIS] [PI12/00523]; FICYT [COF13-12]; FPU grant from the Spanish Ministerio de Educacion, Cultura y Deporte

This work was supported by European Union FEDER funds and "Fondo de Investigación Sanitaria" (FIS, PI12/00523 and PI16/0011; ISCIII, Spain). JR-C is supported by a postdoctoral contract from the "Juan de la Cierva" program (FJCI-2015-23849; MICINN, Spain).

Intestinal dysbiosis, characterized by a reduced Firmicutes/Bacteroidetes ratio, has been reported in systemic lupus erythematosus (SLE) patients. In this study, in vitro cultures revealed that microbiota isolated from SLE patient stool samples (SLE-M) promoted lymphocyte activation and Th17 differentiation from naïve CD4+ lymphocytes to a greater extent than healthy control-microbiota. Enrichment of SLE-M with Treg-inducing bacteria showed that a mixture of two Clostridia strains significantly reduced the Th17/Th1 balance, whereas Bifidobacterium bifidum supplementation prevented CD4+ lymphocyte over-activation, thus supporting a possible therapeutic benefit of probiotics containing Treg-inducer strains in order to restore the Treg/Th17/Th1 imbalance present in SLE. In fact, ex vivo analyses of patient samples showed enlarged Th17 and Foxp3+ IL-17+ populations, suggesting a possible Treg-Th17 trans-differentiation. Moreover, analyses of fecal microbiota revealed a negative correlation between IL-17+ populations and Firmicutes in healthy controls, whereas in SLE this phylum correlated directly with serum levels of IFNγ, a Th1 cytokine slightly reduced in patients. Finally, the frequency of Synergistetes, positively correlated with the Firmicutes/Bacteroidetes ratio in healthy controls, tended to be reduced in patients when anti-dsDNA titers were increased and showed a strong negative correlation with IL-6 serum levels and correlated positively with protective natural IgM antibodies against phosphorylcholine. ; This work was supported by European Union FEDER funds, Fondo de Investigación Sanitaria (PI12/00523), Spanish Plan Nacional de I+D (AGL2010-14952 and AGL2013-44039-R) and Fundación para el Fomento en Asturias de la Investigación Científica Aplicada y la Tecnología (EQUIP09-19). J.R.-C. is a recipient of a FPU grant from the Spanish Ministerio de Educación, Cultura y Deporte. B.J. and A.H. are recipients of a Ramón y Cajal postdoctoral contract and a FPI grant, respectively, both from the Spanish Ministerio deEconomía y Competitividad. We thank SLE patients and ALAS (Asociación Lúpicos de Asturias) for their continuous encouragement. ; Peer reviewed

This work was supported by European Union FEDER funds, Fondo de Investigación Sanitaria (PI16/00113; ISCIII, Spain) and SER/FER funds (Sociedad Española de Reumatología, FER043/2016). JR-C is supported by a postdoctoral contract from the Juan de la Cierva program (FJCI-2015-23849; MINECO, Spain) at FINBA (Fundación para Investigación y la Innovación Biosanitaria del Principado de Asturias).

This work was supported by European Union FEDER funds and "Fondo de Investigacion Sanitaria" (FIS, PI12/00523 and PI16/00113; ISCIII, Spain), and SER/FER funds (Sociedad Espanola de Reumatologia, FER043/2016). JR-C is supported by a postdoctoral contract from the "Juan de la Cierva" program (FJCI-2015-23849; MINECO, Spain).

This work was supported by European Union FEDER funds and Fondo de Investigaciones Sanitarias PI12/00523 MECD AP2010-1614 Sociedad Española de Reumatologia (ES). J.R.-C. is a recipient of a FPU grant from the Ministerio de Educación (AP2010-1614)

This work was supported by European Union FEDER funds, Fondo de Investigación Sanitaria (PI12/00523), Spanish Plan Nacional de I+D (AGL2010-14952 and AGL2013-44039-R) and Fundación para el Fomento en Asturias de la Investigación Científica Aplicada y la Tecnología (EQUIP09-19). J.R.-C. is a recipient of a FPU grant from the Spanish Ministerio de Educación, Cultura y Deporte. B.J. and A.H. are recipients of a Ramón y Cajal postdoctoral contract and a FPI grant, respectively, both from the Spanish Ministerio deEconomía y Competitividad. We thank SLE patients and ALAS (Asociación Lúpicos de Asturias) for their continuous encouragement.

This work was supported by European Union FEDER funds, Fondo de Investigación Sanitaria (PI14/01452, PI16/00113, PI17/02181, PI19/00532) from Instituto de Salud Carlos III (ISCIII), Plan de Ciencia, Tecnología e Innovación 2013-2017 del Principado de Asturias (GRUPIN14-028 and IDI/2018/000152) from Fundación para el Fomento en Asturias de la Investigación Científica Aplicada y la Tecnología (FICYT), Red de Investigación Renal-RedInREN (RETIC REDINREN RD16/0009) from ISCIII, and by Sociedad Asturiana Fomento Investigaciones Metabólicas (SAFIM). JR-C is supported by postdoctoral contracts from the Juan de la Cierva (IJCI-2017-32070, Ministerio de Ciencia e Innovación, Spain) and Sara Borrell programs (CD19/00120, from ISCIII). NC-L is supported by GRUPIN14-028 and IDI/2018/000152, and AD is supported by Asociación Investigación de Fisiología Aplicada and ISPA. BM-C was supported by ISCIII-ISPA (PI17/00384) and by a graduate fellowship from the Gobierno del Principado de Asturias (Severo Ochoa program BP19-057).

This work was supported by European Union FEDER funds and Fondo de Investigación Sanitaria (FIS, PI12/00523 and PI16/00113; ISCIII, Spain), and SER/FER funds (Sociedad Española de Reumatología, FER043/2016). JR-C is supported by a postdoctoral contract from the Juan de la Cierva program (FJCI-2015-23849; MINECO, Spain).

This work was supported by European Union FEDER funds and the Fondo de Investigación Sanitaria (FIS, PI12/00523). JR-C is a recipient of a FPU grant from the Ministerio de Educación (grant number AP2010-1614).

A growing body of evidence highlights the relevance of free fatty acids (FFA) for human health, and their role in the cross talk between the metabolic status and immune system. Altered serum FFA profiles are related to several metabolic conditions, although the underlying mechanisms remain unclear. Recent studies have highlighted the link between gut microbiota and host metabolism. However, although most of the studies have focused on different clinical conditions, evidence on the role of these mediators in healthy populations is lacking. Therefore, we have addressed the analysis of the relationship among gut microbial populations, short-chain fatty acid (SCFA) production, FFA levels, and immune mediators (IFNγ, IL-6, and MCP-1) in 101 human adults from the general Spanish population. Levels of selected microbial groups, representing the major phylogenetic types present in the human intestinal microbiota, were determined by quantitative PCR. Our results showed that the intestinal abundance of Akkermansia was the main predictor of total FFA serum levels, displaying a negative association with total FFA and the pro-inflammatory cytokine IL-6. Similarly, an altered FFA profile, identified by cluster analysis, was related to imbalanced levels of Akkermansia and Lactobacillus as well as increased fecal SCFA, enhanced IL-6 serum levels, and higher prevalence of subclinical metabolic alterations. Although no differences in nutritional intakes were observed, divergent patterns in the associations between nutrient intakes with intestinal microbial populations and SCFA were denoted. Overall, these findings provide new insights on the gut microbiota–host lipid metabolism axis and its potential relevance for human health, where FFA and SCFA seem to play an important role. ; This work was funded through the Grant GRUPIN14-043 "Microbiota Humana, Alimentación y Salud" funded by "Plan Regional de Investigación del Principado de Asturias," Asturias, Spain and by the grants AGL2010-14952 from Spanish "Plan Nacional I+D+I" and by Biopolis SL within the framework of the e-CENIT Project SENIFOOD from the Spanish Ministry of Science and Innovation. JR-C and NS benefit from postdoctoral contracts supported by the Grant GRUPIN14-043 and by a Clarín regional contract cofinanced by the Marie Curie CoFund European Program, respectively. Regional grants received cofounding from European Union FEDER funds. ; The authors acknowledge support of the publication fee by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). ; Peer reviewed

Supported in part by funds from the European Union FEDER, the Fondo de Investigación Sanitaria (grants PI12/00523 and PI12/00060 from the Instituto de Salud Carlos III, Spanish Ministry of Health), and a RETICS program grant (RD12/0009 from the Instituto de Salud Carlos III, Spanish Ministry of Health). Dr. Rodríguez-Carrio's work was supported by an FPU fellowship from the Spanish Ministry of Education (AP2010-1614). Dr. López-Mejías is recipient of a Sara Borrell postdoctoral fellowship from the Instituto de Salud Carlos III at the Spanish Ministry of Health (CD12/00425).